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SPDP-dPEG®₈-NHS ester

SPDP-dPEG®₈-NHS ester

TFP_Esters_Art2SPDP-dPEG®8-NHS ester, product number 10376, is Quanta BioDesign's water-soluble version of the immensely popular SPDP crosslinker. SPDP-dPEG®8-NHS ester is a heterobifunctional crosslinker. The N-hydroxysuccinimidyl (NHS) ester end of the molecule reacts with free amines. The opposite end is a 3-(2-pyridyldithio)-propionate functional group that reacts with sulfhydryl groups in thiol-containing molecules to form disulfide bonds. Between the two functional groups is a water-soluble, single molecular weight, discrete polyethylene glycol (dPEG®) spacer that is 32 atoms (37.2 Å) long.
What makes dPEG® crosslinkers different from other crosslinkers?Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. The crosslinking product N-succinimidyl 3-(2-pyridyldithio)-propionate, known commonly by the acronym SPDP, is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.Quanta BioDesign's amphiphilic dPEG® products create water-soluble conjugates. Our dPEG® products have all the benefits of traditional PEG products (including water solubility, increased hydrodynamic volume, and non-immunogenicity) without the liability of dispersity, which creates difficulties in analyzing the reaction products. To learn more about our dPEG® products and technology, please visit the following pages:What is dPEG®?Frequently Asked Questions (about dPEG® products)How do I use SPDP-dPEG®8-NHS ester?SPDP-dPEG®8-NHS ester, product number 10376, is used like SPDP and related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product does not cause the compounds to which it is conjugated to precipitate due to excessive hydrophobicity. The long spacer arm may facilitate the reaction of the SPDP end of the molecule with buried thiol groups.Greg Hermanson's Bioconjugate Techniques, 3rd edition, describes SPDP and related products (Chapter 6) and describes Quanta BioDesign's dPEG® versions of these products (Chapter 18). Each of the chapters describing these crosslinkers contains detailed protocols for using them. Quanta BioDesign highly recommends and sells this book. Click here now for a review and to purchase Bioconjugate Techniques, 3rd edition.Can I buy SPDP-dPEG®8-NHS ester in bulk?Yes, you can. If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.Buy it now!Stop using inferior hydrophobic SPDP products to crosslink amine and sulfhydryl groups. Click on the "Add to Cart" button today and start using our superior, water-soluble SPDP dPEG® product. You, too, can see "the dPEG® difference."
SPDP-dPEG®₃₆-NHS ester

SPDP-dPEG®₃₆-NHS ester

TFP_Esters_Art2SPDP-dPEG®36-NHS ester, product number 10867, is an amphiphilic, water-soluble, monodispersed PEGylation reagent that is designed to crosslink amines and free thiols. SPDP (sometimes referred to as orthopyridyl disulfide, or OPSS, in the scientific literature) reacts with sulfhydryl groups to form disulfide bonds. The N-hydroxysuccinimidyl (NHS) ester reacts with free amines to form amide bonds. The single molecular weight, discrete chain length polyethylene glycol (dPEG®) chain is 115 atoms (137.8 Å) long.
PEGylation with Quanta BioDesign's dPEG® ProductsQuanta BioDesign's single molecular weight, discrete chain length PEGylation reagents are quite different from traditional PEGylation reagents. Our products are not dispersed polymers, nor are they purified from polymeric mixtures. Instead, we synthesize all of our products from high-purity starting materials and maintain discrete chain lengths and single molecular weights throughout the entire synthetic process. To learn more about our products and technology, please visit our informational pages linked below.What is dPEG®?Frequently Asked Questions (about dPEG® products)Crosslinking with SPDPAmong the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate, also called OPSS) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.Using SPDP-dPEG®36-NHS esterSPDP-dPEG®36-NHS ester, product number 10867, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product prevents hydrophobicity-triggered precipitation of compounds to which it is conjugated. Greg Hermanson's Bioconjugate Techniques, 3rd edition, describes SPDP and related products (Chapter 6) and describes Quanta BioDesign's dPEG® versions of these products (Chapter 18). Each of the chapters describing these crosslinkers contains detailed protocols for using them. Quanta BioDesign highly recommends and sells this book. Click here for a review and to purchase Bioconjugate Techniques, 3rd edition.Bulk Scale Production is AvailableIf you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.Buy Now!If you need to conjugate amine and sulfhydryl groups, stop using inferior hydrophobic SPDP products. Click on the "Add to Cart" button today and start using our superior, water-soluble SPDP-dPEG®36-NHS ester product. You, too, can experience "the dPEG® difference" that thousands of other satisfied customers have enjoyed.
SPDP-dPEG®₄-NHS ester

SPDP-dPEG®₄-NHS ester

TFP_Esters_Art2SPDP-dPEG®4-NHS ester, product number 10374, is Quanta BioDesign's water-soluble version of the immensely popular SPDP crosslinker. SPDP-dPEG®4-NHS ester is a heterobifunctional crosslinker. The N-hydroxysuccinimidyl (NHS) ester end of the molecule reacts with free amines. The opposite end is a 3-(2-pyridyldithio)-propionate functional group that reacts with sulfhydryl groups in thiol-containing molecules to form disulfide bonds. Between the two functional groups is an amphiphilic, single molecular weight, discrete polyethylene glycol (dPEG®) spacer that is 20 atoms (23.1 Å) long.
What is dPEG®?Quanta BioDesign's amphiphilic dPEG® PEGylation reagents create water-soluble conjugates. Our dPEG® products have all the benefits of traditional PEG products (including water solubility, increased hydrodynamic volume, and non-immunogenicity) without the liability of dispersity, which creates difficulties in analyzing the reaction products. This link thoroughly explains the benefits of dPEG® technology. For answers to our most frequently asked questions, please click here.Crosslinking with SPDPAmong the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.Using SPDP-dPEG®4-NHS esterSPDP-dPEG®4-NHS ester, product number 10374, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product will not cause the compounds to which it is conjugated to precipitate due to excessive hydrophobicity. Greg Hermanson's Bioconjugate Techniques, 3rd edition, describes SPDP and related products (Chapter 6) and describes Quanta BioDesign's dPEG® versions of these products (Chapter 18). Each of the chapters describing these crosslinkers contains detailed protocols for using them. Quanta BioDesign highly recommends and sells this book. Click here for a review and to purchase Bioconjugate Techniques, 3rd edition.Bulk Scale Production is AvailableIf you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.Buy Now!If you need to conjugate amine and sulfhydryl groups, quit using inferior hydrophobic SPDP products. Click on the "Add to Cart" button today and start using our superior, water-soluble SPDP dPEG® product. You, too, can see "the dPEG® difference."
SPDP-dPEG®₃₆-acid

SPDP-dPEG®₃₆-acid

SPDP-dPEG®36-acid, product number 10866, is a crosslinking reagent from Quanta BioDesign designed to conjugate free amines and sulfhydryls. The single molecular weight (i.e., monodispersed) PEG crossbridge between the two functional groups is 115 atoms (137.8 Å) long. Because the PEG chain length is discrete, Quanta BioDesign sells this product under the dPEG® tradename. Using a suitable carbodiimide, such as EDC, the propionic acid group reacts with free primary amines to form amide bonds. The pyridyl disulfide (SPDP, also known as OPSS) functional group reacts with free thiols to form disulfide bonds. This product is useful for crosslinking peptides, proteins, and small molecules, and modifying surfaces with suitable reactive sites.
PEGylation with Quanta BioDesign's dPEG® ProductsQuanta BioDesign's dPEG® PEGylation reagents are quite different from traditional PEGylation reagents. Our products are not dispersed polymers, nor are they purified from polymeric mixtures. Instead, we synthesize all of our products from high-purity starting materials and maintain discrete chain lengths and single molecular weights throughout the entire synthetic process. To learn more about our products and technology, please visit our informational pages linked below.What is dPEG®?Frequently Asked Questions (about dPEG® products)Crosslinking with SPDPAmong the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate), also called OPSS, is one of the most popular versions of this type of crosslinker. Unfortunately, the most popular varieties of SPDP (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.Using SPDP-dPEG®36-AcidSPDP-dPEG®36-Acid, product number 10866, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product prevents hydrophobicity-triggered precipitation of compounds to which it is conjugated. Greg Hermanson's Bioconjugate Techniques, 3rd edition, describes SPDP and related products (Chapter 6) and describes Quanta BioDesign's dPEG® versions of these products (Chapter 18). Each of the chapters describing these crosslinkers contains detailed protocols for using them. Quanta BioDesign highly recommends and sells this book. Click here for a review and to purchase Bioconjugate Techniques, 3rd edition.Bulk Scale Production is AvailableIf you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.Buy Now!If you need to conjugate amine and sulfhydryl groups, stop using inferior hydrophobic SPDP products. Click on the "Add to Cart" button today and start using our superior, water-soluble SPDP-dPEG®36-Acid product. You, too, can experience "the dPEG® difference" that thousands of other satisfied customers have enjoyed.
SPDP-dPEG®₁₂-NHS ester

SPDP-dPEG®₁₂-NHS ester

TFP_Esters_Art2SPDP-dPEG®12-NHS ester, product number 10378, is Quanta BioDesign's water-soluble version of the immensely popular SPDP crosslinker. SPDP-dPEG®12-NHS ester is a heterobifunctional crosslinker. The N-hydroxysuccinimidyl (NHS) ester end of the molecule reacts with free amines. The opposite end is a 3-(2-pyridyldithio)-propionate functional group that reacts with sulfhydryl groups in thiol-containing molecules to form disulfide bonds. Between the two functional groups is an amphiphilic, single molecular weight, discrete polyethylene glycol (dPEG®) spacer that is 44 atoms (51.3 Å) long.
The dPEG® Difference in CrosslinkersAmong the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. The crosslinking product N-succinimidyl 3-(2-pyridyldithio)-propionate, known commonly by the acronym SPDP, is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.Quanta BioDesign's amphiphilic dPEG® products create water-soluble conjugates. Our dPEG® products have all the benefits of traditional PEG products (including water solubility, increased hydrodynamic volume, and non-immunogenicity) without the liability of dispersity, which creates difficulties in analyzing the reaction products. For a thorough explanation of dPEG® technology, please click this link. For answers to our most frequently asked questions, please click here.Using SPDP-dPEG®12-NHS ester in Bioconjugation ReactionsSPDP-dPEG®12-NHS ester, product number 10378, is used like SPDP and related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product does not cause the compounds to which it is conjugated to precipitate due to excessive hydrophobicity. The long spacer arm may facilitate the reaction of the SPDP end of the molecule with buried thiol groups.Greg Hermanson's Bioconjugate Techniques, 3rd edition, describes SPDP and related products (Chapter 6) and describes Quanta BioDesign's dPEG® versions of these products (Chapter 18). Each of the chapters describing these crosslinkers contains detailed protocols for using them. Quanta BioDesign highly recommends and sells this book. Click here now for a review and to purchase Bioconjugate Techniques, 3rd edition.Bulk Quantities of SPDP-dPEG®12-NHS ester Are AvailableIf you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.Buy It Now!Stop using inferior hydrophobic SPDP products to crosslink amine and sulfhydryl groups. Click on the "Add to Cart" button today and start using our superior, water-soluble SPDP dPEG® product. You, too, can see "the dPEG® difference."
SPDP-dPEG®₂₄-NHS ester

SPDP-dPEG®₂₄-NHS ester

TFP_Esters_Art2SPDP-dPEG®24-NHS ester, product number 10379, is an amphiphilic, water-soluble, monodispersed PEGylation reagent that is designed to crosslink amines and free thiols. SPDP (sometimes referred to as orthopyridyl disulfide, or OPSS, in the scientific literature) reacts with sulfhydryl groups to form disulfide bonds. The N-hydroxysuccinimide (NHS) ester reacts with free amines to form amide bonds. The single molecular weight, discrete chain length polyethylene glycol (dPEG®) chain is 80 atoms (97.3 Å) long.
PEGylation with Quanta BioDesign's dPEG® ProductsQuanta BioDesign's single molecular weight, discrete chain length PEGylation reagents are quite different from traditional PEGylation reagents. Our products are not dispersed polymers, nor are they purified from polymeric mixtures. Instead, we synthesize all of our products from high-purity starting materials and maintain discrete chain lengths and single molecular weights throughout the entire synthetic process. To learn more about our products and technology, please visit our informational pages linked below.What is dPEG®?Frequently Asked Questions (about dPEG® products)Crosslinking with SPDPAmong the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate, also called OPSS) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.Using SPDP-dPEG®24-NHS esterSPDP-dPEG®24-NHS ester, product number 10379, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. This water-soluble dPEG® product prevents precipitation of conjugates due to excessive hydrophobicity. Greg Hermanson's Bioconjugate Techniques, 3rd edition, describes SPDP and related products (Chapter 6) and describes Quanta BioDesign's dPEG® versions of these products (Chapter 18). Each of the chapters describing these crosslinkers contains detailed protocols for using them. Quanta BioDesign highly recommends and sells this book. Click here for a review and to purchase Bioconjugate Techniques, 3rd edition.Bulk Scale Production is AvailableIf you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.Buy Now!If you need to conjugate amine and sulfhydryl groups, stop using inferior hydrophobic SPDP products. Click on the "Add to Cart" button today and start using our superior, water-soluble SPDP-dPEG®24-NHS ester product. You, too, can experience "the dPEG® difference" that thousands of other satisfied customers have enjoyed.
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