Biocompatible Antibody Conjugation

Secondary antibodies that are modified, labeled, or conjugated using our SuperHydrophilic AQ technology have significant advantages over traditional antibody reagents.  AQ antibody conjugates become more water-soluble with greater biocompatibility than ever before. They also display lower background and higher specific signals in assays.  In addition, they remain stable in solution without aggregating, precipitating, or losing activity.  For the first time, biotinylated antibodies can have full activity in binding streptavidin conjugates without encountering stability issues.  With AQ reagents, antibodies can also be modified to a higher degree of labeling (DOL) to result in greater activity per antibody molecule than is possible with other antibody reagents.

Prior to AQ technology, virtually all antibody conjugates were made—and are still being made—using sticky hydrophobic bioconjugation reagents.  Using these old reagents, antibodies become partially denatured with lower melt temperatures and less solubility.  This problem is present in antibody conjugates made by every other manufacturer selling biotinylated antibodies, antibody-enzyme conjugates, and fluorescently-labeled antibodies.  In every case, the hydrophobic reagents that modify the surfaces of these conjugates become the primary cause of antibody instability.  This often leads to antibody aggregation, precipitation, and loss of activity over time.  Antibody conjugates made with hydrophobic reagents also can have a high background and off-target effects in detection and assay applications.  Every hydrophobic modification done on the surface of antibodies results in the potential to interact nonspecifically with biomolecules, cells, tissues, and surfaces.

Leave hydrophobic antibody conjugates behind and step up to the superior Secondary Antibody conjugates made using our SuperHydrophilic AQ technology.  There is nothing else like them for sensitive detection and assay applications!