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Building Blocks for Peptide Synthesis

Four (4) wooden tiles with black letters that spell out dPEG®, which is Quanta BioDesign's trademark for single molecular weight polyethylene glycol derivatives. These wooden tiles represent the building blocks that dPEG® products provide in peptide synthesis.

Quanta BioDesign’s building blocks and protected linkers enable SuperHydrophilic™ constructs and peptide modifications via our world-famous dPEG® products. Our simple-to-use products transform hydrophobic peptides into hydrophilic products. Moreover, the single molecular weight, discrete-length PEG chain (i.e., dPEG®) simplifies peptide analysis compared to dispersed PEG linkers that contain a range of PEG chain lengths and molecular weights.

We designed these compounds to work in solid-phase and solution-phase peptide synthesis without modification to existing protocols and for other applications where one end of the dPEG® spacer must be protected while the other end reacts. We offer Fmoc-, Cbz-, and boc- (t-boc-) protected linkers and spacers. Moreover, we offer novel constructs that facilitate the easy incorporation of hydrophilic biotin spacers into a growing peptide chain.

We invite you to review our products and see how we have conveniently designed chemistry into biology for powerful biotechnology and life sciences applications. However, if you want to jump to a specific group of products, please click the links below, and we’ll take you there.

Fmoc-protected Building Blocks

CBZ- and t-boc-protected amino-dPEG®x-acid Building Blocks

Other dPEG® Building Blocks and Protected Linkers

Fmoc-Protected Building Blocks for Solid-Phase Peptide Synthesis

Fmoc-protected amino acids are popular reagents in solid-phase peptide synthesis. By design, our Fmoc-N-amido-dPEG®x acids and active esters incorporate directly into solid-phase peptide synthesis with no modification to existing protocols. Furthermore, these protected linkers work in solution-phase synthesis just like protected amino acids. Try them today and discover the dPEG® difference!

 

Fmoc-N-amido-dPEG®x-acid

 

General scheme of Quanta BioDesign's Fmoc-dPEG®x-acid building blocks used in peptide synthesis. The scheme shown here is for solid-phase peptide synthesis, but these building blocks also work well in solution-phase peptide synthesis. In this scheme, a growing peptide chain on a solid-phase support is coupled to an Fmoc-dPEG®x-acid product from Quanta BioDesign. Piperidine deprotects the Fmoc group, which allows the dPEG® spacer to be used as an end group for further modification, such as the addition of labels (e.g., dyes) or small molecule drugs. However, additional peptide synthesis can follow the Fmoc deprotection step, resulting in the dPEG® spacer being used to impart hydrophilicity and flexibility to a peptide chain in the middle of the chain.
Quanta BioDesign’s Fmoc-dPEG®x-acid building blocks facilitate the synthesis of hydrophilic peptides. These discrete PEG (dPEG®) can be used as spacers within a peptide sequence to add flexibility and hydrophilicity or as end groups for hydrophilicity and further modification, such as the addition of labels (e.g., dyes) or small molecule drugs.

 

Fmoc-protected amino-dPEG®x-acid products can be incorporated at either end or in the middle of a peptide chain or added to an amine-functionalized amino acid side chain.

Incorporating dPEG® linkers and spacers into peptides adds hydrodynamic volume, increases hydrophilicity, and decreases or eliminates immunogenicity.

These linkers are available in ten (10) different sizes from dPEG®2 to dPEG®48 (as a dPEG®24-amido-dPEG®24-acid) to cover almost every customer need.

Click this link to view and buy these products.

 

Fmoc-N-amido-dPEG®x-NHS ester

 

Our N-Fmoc-amido-dPEG®x-NHS esters are preactivated, Fmoc-protected amino-dPEG® acid building blocks for peptide synthesis, where the active ester is made from N-hydroxysuccinimide (NHS). This general application scheme shows how to use them in peptide synthesis on solid supports. They also work well in solution-phase peptide synthesis.
Our high-purity N-hydroxysuccinimide (NHS) esters of N-Fmoc-amido-dPEG®x-acid building blocks enable novel peptide constructs that are hydrophilic, non-immunogenic, and flexible (if the dPEG® spacer is in the middle of the peptide). Build novel, perfect peptides with our N-Fmoc-amido-dPEG®x-NHS ester building blocks.

 

Fmoc-N-amido-dPEG®x-NHS ester products have all the benefits of our Fmoc-N-amido-dPEG®x-acid products; Preactivating the carboxyl terminus with N-hydroxysuccinimide (NHS) simplifies couplings to free amines.

Available in three highly popular linker lengths: dPEG®4, dPEG®8, and dPEG®12.

Click here to see and purchase these products and discover the dPEG® difference.

 

Fmoc-N-amido-dPEG®x-TFP ester

 

Our N-Fmoc-amido-dPEG®x-TFP esters are preactivated, Fmoc-protected amino-dPEG® acid building blocks for peptide synthesis, where the active ester is made from 2,3,5,6-tetrafluorophenol. This general application scheme shows how to use them in peptide synthesis on solid supports. They also work well in solution-phase peptide synthesis.
Our N-Fmoc-amido-dPEG®x-TFP esters are Fmoc-protected amino-dPEG® acid building blocks for peptide synthesis. They come preactivated as 2,3,5,6-tetrafluorophenyl (TFP) esters. The TFP esters provide greater hydrolytic stability and amine selectivity, making them highly useful in challenging peptide syntheses. They work well in solid-phase and solution-phase peptide synthesis reactions and do not require modification to existing protocols.

 

Fmoc-N-amido-dPEG®x-TFP ester products have all the benefits of our Fmoc-N-amido-dPEG®x-acid products and the same reactivity as our Fmoc-N-amido-dPEG®x-NHS ester products.

TFP esters react with amines like NHS esters, are more resistant to hydrolysis than NHS esters, have higher selectivity than NHS esters in solution, and may provide superior reactivity with amines for challenging conjugations.

Available in five (5) different lengths from dPEG®4 to dPEG®36.

Click this link to see all of our Fmoc-N-amido-dPEG®x-TFP ester products.

To learn more about TFP esters as an alternative to NHS esters, please click here.

 

Fmoc-N-amido-Lys(dPEG®x-biotin)-OH (acid)

 

General scheme of Quanta BioDesign's Fmoc-N-Lys(dPEG®x-Biotin)-OH (acid) building blocks. These peptide building blocks facilitate incorporating a dPEG®-biotin label at the N-terminus, middle, or C-terminus of a peptide chain while using solid- or solution-phase peptide synthesis. In the example shown here, the dPEG®-biotin spacer is added to the N-terminus of a peptide chain through solid-phase peptide synthesis.
Add a dPEG®-biotin label to a peptide chain using Quanta BioDesign’s Fmoc-N-Lys(dPEG®x-Biotin)-OH (acid) products! These unique products facilitate incorporating a hydrophilic biotin label into a peptide chain anywhere you want it. These compounds work in solid-phase and solution-phase peptide synthesis and come in two chain lengths: dPEG®4 and dPEG®12.

 

A unique peptide building block from Quanta BioDesign!

This product incorporates a flexible, hydrophilic dPEG®-biotin group on the side chain of Fmoc-protected lysine.

The lysine can be added anywhere along the peptide chain, although the N-terminus is the preferred location. The resulting product is a biotinylated peptide that is useful for a variety of applications that take advantage of the strong binding between biotin and (strept)avidin.

The hydrophilic dPEG® spacer improves the hydrophilicity and reduces the immunogenicity of the final peptide product.

Two linker sizes (4 and 12) are available and ready for use.

Click here to see and buy both products.

CBZ- and t-boc-protected amino-dPEG®x-acid Building Blocks

 

Benzyl carbamates (CBZ-protected amines) and tert-butyl carbamates (BOC-protected amines) are useful in solution-phase peptide synthesis and many other synthetic reactions. They are also among the most popular amine protecting groups currently used. The protecting groups shield the amine from reacting while the carboxyl end of the dPEG® linker is free to react with amines to form amide bonds.

Our dPEG® linkers cover a broad range of chain lengths. Each high-purity product contains a single molecular weight PEG with a discrete chain length, thus simplifying the analysis of the resulting conjugate. We invite you to try our products today and discover the dPEG® difference.

 

t-boc-N-amido-dPEG®x-acid

 

This image shows a solid-phase peptide synthesis application using Quanta BioDesign's t-boc-N-amido-dPEG®x-acid building blocks.
Quanta BioDesign offers BOC-protected dPEG® building blocks for solid- and solution-phase peptide synthesis. These products can be used as peptide end-group modifiers and solubility enhancers or as spacers within a sequence to add flexibility to the peptide backbone or control functionality and reactivity of peptide-dPEG® conjugates.

 

The BOC group is the most widely used protecting group for amines.

It is stable to base but cleaves easily and cleanly with trifluoroacetic acid (TFA), yielding only CO2 and tert-butanol or isobutylene as by-products.

We offer five (5) high-purity products ranging from dPEG®4 to dPEG®36.

Click this link to view and buy these products.

 

CBZ-N-amido-dPEG®x-acid

 

Quanta BioDesign's N-CBZ-amido-dPEG®x-acid products are carboxybenzyl-protected amino-dPEG® acid building blocks for peptide synthesis. This general schematic shows how to use them to design hydrophilic, non-immunogenic, flexible peptides. These compounds work well in solid-phase and solution-phase peptide synthesis.
Our N-CBZ-amido-dPEG®x-acid products are building blocks for peptide synthesis that work well in solid-phase and solution-phase peptide synthesis. After deprotection, peptides are hydrophilic and flexible, and the dPEG® spacers are non-immunogenic.

 

The CBZ group provides stable protection for amines under a wide variety of conditions.

CBZ groups deprotect cleanly under various mild conditions. A common method of cleaving the CBZ protecting group is hydrogenation with a metal catalyst such as palladium on carbon. However, many different deprotection methods are published.

We offer seven (7) products ranging in size from dPEG®4 to dPEG®24-amido-dPEG®24 (i.e., a dPEG®48).

Click here to see and purchase these products.

Other dPEG® Building Blocks and Protected Linkers

Methoxytrityl-S-dPEG®x-acid

 

An example of how to use Methoxytrityl-S-dPEG®x building blocks in an application. In the example shown, a peptide sequence of variable size and structure is coupled to a solid phase support. Mmt-S-dPEG®x-COOH (where x = 4, 8, or 12) is activated and coupled to the peptide. A subsequent deprotection step (TFA + TES or TIS) removes the methoxytrityl group, and then the peptide is decoupled from the solid phase support. The methoxytrityl-S products offered by Quanta BioDesign are building blocks that add a non-immunogenic, hydrophilic spacer at the end or on the side chain of a peptide, converting an amine group to a thiol. There are many uses for this tool.
Quanta BioDesign’s Mmt-S-dPEG®x-COOH products are building blocks for adding thiols to the N-termini or N-side chains of amino acids in peptide chains.

 

These SuperHydrophilic building blocks are tools that add a dPEG® linker with a terminal thiol to an amino group. They can be used at the N-termini of peptides or the N-side chains of amino acid residues within the chain.

The resulting conjugate can modify physical properties or introduce a spacer end group.

The methoxytrityl group cleaves with TFA in the presence of a scavenger such as TES or TIS.

Three sizes of dPEG® linker are available: dPEG®4, dPEG®8, and dPEG®12.

Click here to view and purchase these products.

 

Amino-dPEG®12-ODMT for Nucleic Acids, Peptides, and SuperHydrophilic Constructs

 

 

This application graphic shows PN10342, Amino-dPEG®12-ODMT, one of Quanta BioDesign's versatile building blocks and protected linkers, being used to modify an oligonucleotide. The free amine end of PN10342 couples to a 5' phosphate group on an oligonucleotide by using a combination of EDC•HCL and imidazole. The dimethoxytrityl (DMT) group removes cleanly using 80% aqueous acetic acid, exposing the terminal hydroxy of the discrete PEG spacer. Further modification can occur at this point, if required.
Build better aptamers with Quanta BioDesign’s Amino-dPEG®12-ODMT, product number 10342, building block! This product can modify 5′ phosphate groups of oligonucleotides, providing a discrete PEG spacer to modify physical properties or to facilitate other oligonucleotide modifications such as the addition of dyes or other labels. Peptide modification and macromolecular construction applications are also possible.

 

Build better aptamers by incorporating a discrete PEG (dPEG®) spacer into oligonucleotides.

Modify the side chains of acidic amino acids to increase hydrophilicity and add hydrodynamic volume.

Use Amino-dPEG®12-ODMT, product number 10342, in macromolecular constructs to add defined, SuperHydrophilic spacers to the macromolecular architecture.

After reacting the amine end of the dPEG® linker, the dimethoxytrityl protecting group removes easily under mild conditions, exposing the terminal alcohol.

Click here to view and purchase the product.

 

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