Specs:

 SDS

Molecular Weight: 601.60; single compound

CAS: 1137109-21-7

Purity: > 98%

Spacers: dPEG® Spacer is 28 atoms and 31.7 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, as well as his specific discussion with protocols of our MAL-dPEG®x-NHS esters on pp. 718-722.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Direct Cell Surface Modification with DNA for the Capture of Primary Cells and the Investion of Myotube Formation on Defined Patterns. Sonny C. Haiso, Betty J. Shaun, Hiroaki Onoe, Erik S. Douglas, Zev J. Gartner, Richard A. Mathies, Carolyn R. Bertozzi, and Matthew B. Francis. Langmuir. 2009, 25 (12), pp 6985–6991. April 29, 2009. DOI: 10.1021/la900150n.

A sandwich-type DNA array platform for detection of GM targets in multiplex assay. Sena Cansız, Can Ozen, Ceren Bayrac, A. Tahir Bayrac, Fatma Gul, Murat Kavruk, Remziye Yılmaz, Fusun Eyidogan, Huseyin, Avni Oktem. European Food Research and Technology. 2012, 235 (3) pp 429-437. July 6, 2012. DOI:10.1007/s00217-0121767y.

A virus-like particle vaccine platform elicits heightened and hastened local lung mucosal antibody production after a single dose. Laura E. Richert, Amy E. Servidb, Ann L. Harmsen, Agnieszka Rynda-Apple, Soo Han, James A. Wiley, Trevor Douglas, Allen G. Harmsen. Vaccine. 2012, 30 (24) pp 3653-3665. May 21, 2012. DOI: 10.1016/j.vaccine.2012.03.035.

New Assay for Quantification of PEGylated Proteins During in Vitro Permeability Studies. Spela Jalen,Vanja Smilovic, Katarina Fidler, Barbara Podobnik, Maja Marusic, Radovan Komel, Vladka Gaberc-Porekar and Simona Jevsevar. Acta Chimica Slovenica. 2014, 61 (3) pp 615-622. January 15, 2014.

Optimization, Production and Characterization of a CpG-Oligonucleotide-Ficoll Conjugate Nanoparticle Adjuvant for Enhanced Immunogenicity of Anthrax Protective Antigen. Bob Milley, Radwan Kiwan, Gary S. Ott, Carlo Calacsan, Melissa Kachura, John D Campbell, Holger Kanzler, and Robert L. Coffman. Bioconjugate Chemistry. 2016, April 13, 2016. DOI: 10.1021/acs.bioconjchem.6b00107.

Immune Response Modulation of Conjugated Agonists with Changing Linker Length. Keun Ah Ryu, Katarzyna Slowinska, Troy Moore, and Aaron Esser-Kahn. ACS Chemical Biology. 2016, October 17, 2016. DOI: 10.1021/acschembio.6b00895.

Surface Coating of Nanoparticles Reduces Background Inflammatory Activity while Increasing Particle Uptake and Delivery. Brittany A Moser, Rachel C Steinhardt, and Aaron P Esser-Kahn. ACS Biomaterials Science and Engineering. 2016. December 1, 2016. DOI: 10.1021/acsbiomaterials.6b00473.

New Perspectives in the Renin-Angiotensin-Aldosterone System (RAAS) II: Albumin Suppresses Angiotensin Converting Enzyme (ACE) Activity in Human. Miklos Fagyas, Katalin Uri, Ivetta M. Siket, Gabor A. Fulop, Viktoria Csato, Andrea Darago, Judit Boczan, Emese Banyai, Istvan Elek Szentkiralyi, Tamas Miklos Maros, Tamas Szerafin, Istvan Edes, Zoltan Papp, Attila Toth. PLoS ONE. 2014, 9 (4) e87844. April, 1 2014. DOI: 10.1371/journal.pone.0087844.





Additional Search Phrases:

PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, maleimide, maleimido, N-hydroxysuccinimide, NHS, NHS ester, crosslinking, crosslinker, cross linker, heterobifunctional, amine reactive, thiol reactive, PEG6, sulfhydryl reactive, maleimide thiol, PEG thiol, active ester, MAL-PEG6-NHS Ester NHS-PEG6-MAL, Maleimide-PEG6-NHS ester, NHS-PEG6-maleimide, SM(PEG)6, Maleimide-PEG-NHS ester



 SDS    Datasheet

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MAL-dPEG®₆-NHS ester

$185.00 – $300.00

Clear
PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

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MAL-dPEG®6-NHS ester, product number 10064, is a crosslinking reagent that joins a sulfhydryl to a free amine across a hydrophilic bridge. The sulfhydryl groups react with a maleimide group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the N-hydroxysuccinimidyl (NHS) ester of a carboxylic acid group. The maleimide and NHS functional groups on the crosslinking compound sit at either end of a short, discrete-length polyethylene glycol chain (dPEG®).

The reactions that join free amines to free thiols are among the most popular[1], most useful[2] crosslinking reactions in bioconjugate chemistry. These reactions require heterobifunctional reagents that bridge the two different reactive groups. Traditional crosslinkers are hydrophobic molecules. Quanta BioDesign’s dPEG® crosslinking products are single molecular weight PEG compounds with discrete chain lengths.

The conjugation of conventional hydrophobic crosslinking reagents to biomolecules almost inevitably triggers problems such as aggregation and precipitation of the conjugates. These problems do not occur with our water-soluble, non-immunogenic dPEG® crosslinkers. For more information about our dPEG® products, please see our “What is dPEG®?” page. Also, click here for answers to our most frequently asked questions.

With these crosslinkers, the NHS ester end of the molecule must conjugate to a target molecule before the maleimide end of the molecule. At pH 7.0 – 7.5, NHS esters react optimally with free amines. However, NHS esters can react with free amines with pH as low as 6.0. At pH 8 and 25°C, the half-life of NHS esters is one hour or less.[3] As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing MAL-dPEG®6-NHS ester, product number 10064, in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use.

The reaction of the maleimide end of MAL-dPEG®6-NHS ester, product number 10064, with a sulfhydryl proceeds optimally at pH 6.5 – 7.5. Use the lowest reasonable pH within this range. Above pH 7.5, free amines compete with free thiols at the maleimide reaction site, which can cause confusing results. Moreover, at higher pH values, the maleimide ring may open to form unreactive maleamic acid.[4] For details about maleimide-thiol reaction chemistry, please click here.

The use of MAL-dPEG®6-NHS ester, product number 10064, has been published in numerous scientific paper and patents. The following list highlights some of the more important uses of this product:

  • cell surface modification;
  • vaccine development;
  • optimization of a nanoparticle adjuvant;
  • development of DNA assay platforms;
  • measurement of immune system modulation of conjugated agonists;
  • surface coating of nanoparticles; and
  • development of a quantitative assay of PEGylated proteins.

What can you do with this product? Please click the “Add to Cart” button now to order MAL-dPEG®6-NHS ester, product number 10064.

Application References

[1] Hermanson, G. T. Chapter 6, Heterobifunctional Crosslinkers. In Bioconjugate Techniques, 3rd ed.; Academic Press: New York, NY, 2013; pp 299–340, specifically p. 300. Many scientists engaged in bioconjugation work consider Greg Hermanson’s book to be the definitive reference on the subject. Click here now to read a review of Greg’s book and to purchase it.

[2] Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. In Bioconjugate Techniques, 3rd ed.; Academic Press: New York, NY, 2013; pp 787–838, specifically p. 794.

[3] Hermanson, G. T. Chapter 3, The Reactions of Bioconjugation. In Bioconjugate Techniques; Academic Press: New York, NY, 2013; pp 229–258, specifically page 234.

[4] Hermanson, G. T. Chapter 6, op. cit., page 304.

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Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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