Specs:

 SDS

Molecular Weight: 421.46; single compound

CAS: 937025-17-7

Purity: > 98%

Spacers: dPEG® Spacer is 16 atoms and 18.3 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). References to SATA which can be directly applied to the dPEG® pegylation versions: a) general description and use, p 71; b) modification of antibodies, pp 759; c)modification of amines on nucleus and DNA probes, p. 984 and d) modification of enzymes, avidin and streptavidin with pp. 90, 909 and 919.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Elongated plant virus-based nanoparticles for enhanced delivery of thrombolytic therapies. Andrzej Stanislaw Pitek, Yunmei Wang, Sahil Gulati, Huiyun Gao, Phoebe L Stewart, Daniel I. Simon, and Nicole F Steinmetz. Molecular Pharmaceutics. 2017, pp 1-9. September 7, 2017. DOI: 10.1021/acs.molpharmaceut.7b00559.

Biodegradable Polydisulfide Dendrimer Nanoclusters as MRI Contrast Agents. Ching-Hui Huang, Kido Nwe, Ajlan Al Zaki, Martin W. Brechbiel, and Andrew Tsourkas. ACS Nano. 2012, 6 (11) pp 9416–9424. October 25, 2012. DOI: 10.1021/nn304160p.

Silica-Shelled Single Quantum Dot Micelles as Imaging Probes with Dual or Multimodality. Rumiana Bakalova, Zhivko Zhelev, Ichio Aoki, Hideki Ohba, Yusuke Imai, and Iwao Kanno. Anal. Chem. 2006, 78 (16) pp 5925–5932. July 14, 2006. DOI: 10.1021/ac060412b.

Robust IgG responses to nanograms of antigen using a biomimetic lipid-coated particle vaccine. Anna Bershteyn, Melissa C. Hanson, Monica P. Crespo, James J. Moon, Adrienne V. Li, Heikyung Suh, Darrell J. Irvine. Journal of Controlled Release. 2011, 157 (3) pp 354-365. July 24, 2011. DOI: 10.1016/j.jconrel.2011.07.029.

Synthesis and Function of Bioactive, Block Copolymer Surfactant Constructs
as Relevant to the Preparation of Anticoagulant and Antibacterial Medical Implant Surfaces. Pranav R. Joshi. Doctor of Philosophy in Chemical Engineering. 2006, November 17, 2006.

Immobilization of His-tagged endoglucanase on gold via various Ni-NTA self-assembled monolayers and its hydrolytic activity. Nakamura I, Makino A, Ohmae M, Kimura S. Macromolecular Bioscience. 2010, 10 (10) pp1265-1272. October 8, 2010. DOI: 10.1002/mabi.201000189.

Array-based fluorescence assay for serine/threonine kinases using specific chemical reaction. Shoji Akita, Naoki Umezawa, Nobuki Kato, Tsunehiko Higuchi. Bioorganic & Medicinal Chemistry. 2008, 16 (16) pp 7788-94. July 1, 2008. DOI: 10.1016/j.bmc.2008.07.007.

Serum albumin ‘camouflage’ of plant virus based nanoparticles prevents their antibody recognition and enhances pharmacokinetics. Andrzej S. Pitek, Slater A. Jameson, Frank A. Veliz, Sourabh Shukla, and Nicole F. Steinmetz. Biomaterials. 2016, 89 pp 89-97, February 23, 2016. DOI: 10.1016/j.biomaterials.2016.02.032.

Mapping of Molecular Structure of the Nanoscale Surface in Bio-nanoparticles. Luciana M Herda, Delyan R Hristov, Maria Cristina Lo Giudice, Ester Polo, and Kenneth A Dawson. Journal of the American Chemical Society. 2017. 139(1), 111-114. DOI: 10.1021/jacs.6b12297.


Additional Search Phrases:

dPEG4-SATA, S-acetyl-dPEG4-NHS ester, 937025177, PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, SATA, latent thiol, amine reactive, NHS, NHS ester, N-hydroxysuccinimide, succinimidyl ester, chemical modification reagent, PEG4, Acetyl-S-dPEG4-NHS ester, S-acetyl-PEG4-NHS ester, PEG4-SATA, PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, N-Succinimidyl S-acetyl(thiotetraethylene glycol), S-acetyl-PEG-NHS ester



 SDS    Datasheet

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dPEG®₄-SATA (S-acetyl-dPEG®₄-NHS ester)

$235.00 – $1,250.00

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PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses
Product#: 10181 Categories: ,

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dPEG®4-SATA, also known as S-acetyl-dPEG®4-NHS ester, product number 10181, is one of Quanta BioDesign’s version of the widely popular thiolation reagent N-succinimidyl-S-acetylthioacetate (SATA). The acetyl-protected thiol is separated from the N-hydroxysuccinimidyl (NHS) ester by a short, amphiphilic, discrete polyethylene glycol (dPEG®) spacer. The dPEG® spacer adds water solubility to the product.

Quanta BioDesign’s dPEG® Technology

Traditional polyethylene glycol (PEG) products are dispersed polymers (Đ > 1). PEG polymers consist of a mixture of different chain lengths and molecular weights of PEG in a Poisson distribution. Quanta BioDesign manufactures PEG products that are not dispersed (Đ = 1). The PEG contained in every PEG product made by Quanta BioDesign has a single molecular weight with a discrete chain length (hence the tradename dPEG®). For more information about dPEG® technology, please click here. For answers to our most frequently asked questions, please click this link.

Thiolation with dPEG®4-SATA

Thiolation is the process of adding a sulfhydryl group to a molecule. Bioconjugation frequently employs thiolation because the reactions to install thiol groups on molecules or to react molecules with sulfhydryl groups are simple and often chemoselective. For more information, please see Thiolation Reagents and Reactions. {link}

The widely popular SATA reagent thiolates molecules with available amines via the NHS ester. Removal of the acetyl protecting group from the sulfhydryl permits crosslinking between the SATA-modified compound and a target molecule of interest that contains a thiol-reactive group, such as maleimide. Unfortunately, SATA is hydrophobic. Consequently, before use, SATA must be dissolved in a dry, water-miscible organic solvent.

Quanta BioDesign’s dPEG®4-SATA inserts a single molecular weight tetraethylene glycol (dPEG®4) spacer between the protected thiol and the NHS ester. The dPEG® spacer imparts water solubility to the molecule, allowing dPEG®4-SATA to be dissolved and reacted in water without the need for an organic solvent. Also, the dPEG®4 spacer adds hydrodynamic volume to the molecule to which it is conjugated. Increasing the hydrodynamic volume reduces aggregation and precipitation of proteins conjugated to dPEG®4-SATA. Hydroxylamine hydrochloride (CAS number 5470-11-1) easily removes the acetyl protecting group, exposing the sulfhydryl moiety for further reaction.

Any application that can be carried out with a traditional, non-PEGylated SATA reagent can be carried out with our SATA containing a dPEG® spacer. In Bioconjugate Techniques, 3rd edition (see references 1 and 2, below), Greg Hermanson has provided protocols for the thiolation of enzymes, antibodies, avidin, streptavidin, phycobiliproteins, and amine-containing DNA using SATA. All of these protocols can be conducted successfully using dPEG®4-SATA (S-acetyl-dPEG®4-NHS ester) or any of our other SATA reagents.

Commercial Scale Production

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

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Application References:

  1. Hermanson, G. T. Chapter 2, Functional Targets for Bioconjugation. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 127-228, particularly pages 165-170, discussing thiolation and SATA. Want to learn more about Greg’s book? Click here now for a review of Greg’s manual and a link to purchase it.
  2. Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 787-838.

Description

 

 

 

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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