Specs:

 SDS

Molecular Weight: 560.63; single compound

CAS: 1334172-59-6

Purity: > 98%

Spacers: dPEG® Spacer is 15 atoms and 18.1 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 529-530 for a general discussion in Greg’s book and on biotinylation as well, as well as pp. 737-738 for a protocol for a compound with just two oxygen. However, other than enhanced solubility and binding distances, the protocol is adaptable to our PN 10193.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Molecularly Imprinted Polymer Arrays as Synthetic Protein Chips Prepared by Transcription-type Molecular Imprinting by Use of Protein-Immobilized Dots as Stamps. Takahiro Kuwata, Akane Uchida, Eri Takano, Yukiya Kitayama, and Toshifumi Takeuchi. Analytical Chemistry. 2015, 87 (23) pp 11784-11791. November 16, 2015. DOI:10.1021/acs.analchem.5b03134.

Electrogenerated Chemiluminescence. 77. DNA Hybridization Detection at High Amplification with [Ru(bpy)3]­­­2+-Containing Microspheres, Wujian Miao and Allen J. Bard. Anal. Chem. 2004, 76 (18), pp 5379-5386. August 7, 2004. DOI: 10.1021/ac0495236.

Automated flow-through amperometric immunosensor for highly sensitive and on-line detection of okadaic acid in mussel sample. Rocio B. Dominguez, Akhtar Hayat, Audrey Sassolas, Gustavo A. Alonso, Roberto Munoz, Jean-Louis Marty. Elsevier. 2012, (99), pp 232-237, September 15, 2012. DOI: 10.1016/j.talanta.2012.05.045.

KCa3.1 and TRPM7 Channels at the Uropod Regulate Migration of Activated Human T Cells. Zerrin Kuras, Yeo-Heung Yun, Ameet A. Chimote, Lisa Neumeier, Laura Conforti. PLoS ONE 7(8), e43859. August 27, 2012. DOI: 10.1371/journal.pone.0043859.

Synthesis and evaluation of cell-permeable biotinylated PU-H71 derivatives as tumor Hsp90 probes. Tony Taldone, Anna Rodina, Erica M. DaGama Gomes, Matthew Riolo, Hardik J. Patel, Raul Alonso-Sabadell, Danuta Zatorska, Maulik R. Patel, Sarah Kishinevsky and Gabriela Chiosis. Beilstein J. Org. Chem. 2013, 9 pp 544-556. March 15, 2013. DOI: 10.3762/bjoc.9.60.

Electrochemically Directed Modification of ITO Electrodes and Its Feasibility for the Immunosensor Development. Daegeun Yu and Kyuwon Kim. Bull Korean Chem. Soc.2009, 30 (4) pp 955-958. February 23, 2009.

Efficient Short Interference RNA Delivery to Tumor Cells Using a Combination of Octaarginine, GALA and Tumor-Specific, Cleavable Polyethylene Glycol System. Yu SAKURAI, Hiroto HATAKEYAMA, Hidetaka AKITA, Motoi OISHI, Yukio NAGASAKI, Shiro FUTAKI and Hideyoshi HARASHIMA. Biol. Pharm. Bull. 2009, 32 (5) pp 928-932. May 1, 2009. doi:10.1248/bpb.32.928.

Development of Robust and Standardized Cantilever Sensors Based on Biotin/Neutravidin Coupling for Antibody Detection. Jiayun Zhang, Hans Peter Lang, Felice Battiston, Natalija Backmann, Francois Huber and Christoph Gerber. Sensors. 2013, 13 pp5273-5285. April 19, 2013. doi:10.3390/s130405273.

Synthesis of Biotinylated r-D-Mannoside or N-Acetyl _-D-Glucosaminoside Decorated Gold Nanoparticles: Study of Their Biomolecular Recognition with Con A and WGA Lectins. Xiaoze Jiang, Abdelghani Housni, Guillaume Gody, Paul Boullanger, Marie-The´re`se Charreyre, Thierry Delair, and Ravin Narain. Bioconjugate Chem. 2010, 21 pp 521–530. February 3, 2010. DOI: 10.1021/bc900431p.

An Investigation of Phosphorous-Based Bioconjugation Techniques for Protein Modification. Maja Lopandic. UWSPACE. 2022. April 7, 2022. http://hdl.handle.net/10012/18138.


Additional Search Phrases:

Biotin-dPEG3-NH3+TFA, 1334172596, PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, biotin, biotinylation, biotinylation reagent, biotin labeling, biotin label, amine, amino, amino group, carbonyl reactive, carboxyl reactive, TFA, TFA salt, trifluoroacetic acid, trifluoroacetate, Biotin-PEG3-NH3+-TFA-, Biotin-PEG3-amine TFA salt, Biotin-PEG3-ammonium trifluoroacetate, Biotin-PEG-amine



 SDS    Datasheet

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Biotin-dPEG®₃-NH₃+TFA-

$100.00 – $750.00

Clear
PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses
Product#: 10193 Categories: ,

Biotin-dPEG®3-NH3+TFA– (Biotin-dPEG®3-ammonium trifluoroacetate), product number 10193, is one of several biotinylation products offered by Quanta BioDesign, Ltd. The product reacts with activated esters (N-hydroxysuccinimide, 2,3,5,6-tetrafluorophenyl) of carboxylic acids under mildly basic conditions. It can be coupled directly to a carboxylic acid using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry. Also, the product can react with carbonyl groups (aldehydes or ketones) to form a Schiff base. The Schiff base can be reduced to a secondary amine using sodium cyanoborohydride if additional conjugate stability is necessary.

Biotinylation

Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or a surface such as glass or gold. Bioconjugation research, product development, and many types of clinical tests use biotinylated products.

Biotin usually is poorly soluble in water, but the amphiphilic dPEG® linker imparts excellent solubility in water or aqueous buffer and organic solvents. In the past, there have not been many choices for carbonyl- and carboxylic acid-reactive biotinylation reactions. The most popular choices were biotin-hydrazide, biocytin-hydrazide (biotin-lysine hydrazide), and biotin-LC-hydrazide (biotin-aminocaproic acid hydrazide), but all of these choices are unfortunately quite hydrophobic. These older, hydrophobic reagents required solvation in an organic solvent such as N,N’-dimethylformamide (DMF) or dimethylsulfoxide (DMSO) before using in an aqueous buffer. When conjugated to proteins or other biomacromolecules, the hydrophobic interactions caused or exacerbated by these reagents triggered aggregation and precipitation of conjugated molecules and increased background noise through non-specific binding.

Amphiphilic biotin-dPEG®3-NH3+TFA– dissolves equally well in aqueous buffers and organic solvents. There is no need to dissolve this product in an organic solvent before using it in aqueous reaction media. Furthermore, biotinylation of biomacromolecules with PN10193 will not cause aggregation and precipitation. Eliminating aggregation should consequently improve signal-to-noise ratios in applications developed with this product. Reaction with a carboxylic acid or active ester of a carboxylic acid will yield a stable amide bond. Aldehydes are uncommon in biomacromolecules, but they form through the oxidation of reducing sugars in glycosylated proteins. Reactions with aldehydes will produce imines that can be reduced under mild conditions to secondary amines with sodium cyanoborohydride.

Any application that can take advantage of the powerful biotin-avidin/streptavidin affinity can use Biotin-dPEG®3-NH3+TFA–. The terminal amine of PN10193 offers the opportunity to biotinylate (1) the carbohydrate coat of glycoproteins, and (2) surfaces consisting of carboxylic acids or their active esters. Thus, Biotin-dPEG®3-NH3+TFA– expands the range of molecules and nanoparticles for which dPEG®-biotin is useful.

Learn more!

What is dPEG®?

Frequently Asked Questions (about dPEG® products)

Biotinylation with dPEG® Products

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Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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