Specs:

 SDS

Molecular Weight: 922.09; single compound

CAS: 1334172-60-9

Spacers: dPEG® Spacer is 43 atoms and 50.5 Å

References: Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

TCR Triggering by pMHC Ligands Tethered on Surfaces via Poly(Ethylene Glycol) Depends on Polymer Length. Zhengyu Ma, David N. Lebard, Sharon M. Loverde, Kim A. Sharp, Michael L. Klein, Dennis E. Discher, Terri H. Finkel. PLoS ONE. 2014. November 10, 2014. DOI: 10.137/journal.pone.0112292.

A magnetic bead-based protein kinase assay with dual detection techniques. Guangchang Zhou, Juliesta E. Sylvester, Ding Wua, Darren R. Veach , Stephen J. Kron. Analytical Biochemistry. 2011, 1 (408), pp 5-11, January 2011. DOI: 10.1016/j.ab.2010.08.034.

An Assay to Quantitate Reducible Cysteines from Nanograms of GST-Fusion Proteins Dixon J. Woodbury, Chris A. Rees, Ammon Thompson, Paul Meiners, April Adams. Analytical Biochemistry. 2011, 417 (2) pp 165-173. October 15, 2011. DOI: 10.1016/j.ab.2011.06.017.

The rotation-coupled sliding of EcoRV. Jasmina Dikic, Carolin Menges, Samuel Clarke, Michael Kokkinidis, Alfred Pingoud, Wolfgang Wende and Pierre Desbiolles. Nucleic Acids Research. 2012, 40 (9), pp 4064-4070. January 12, 2012. DOI:10.1093/nar/gkr1309.




Additional Search Phrases: Biotin-dPEG11-MAL, maleimide, maleimido, 1334172609, PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, biotin, biotinylation, biotinylation reagent, biotin labeling, biotin label, maleimide, thiol, thiol reactive, sulfhydryl


Biotin-dPEG®₁₁-MAL

$225.00 – $450.00

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Product#: 10195 Categories: , , Tags: , , , , , , , , , , , , , , , , , ,

Biotin-dPEG®11-MAL, product number 10195, is one of three products containing a biotin on one end of a dPEG® linker and a maleimidopropyl moiety on the other end. The maleimide group is a useful, highly popular reactive group for conjugating to free thiol groups in biomolecules. This product provides a short-chain, single molecular weight, discrete PEG (dPEG®) spacer between the biotin and the maleimide. Maleimide groups can be conjugated to free thiol groups in the pH range from 6.5 – 7.5, forming a stable thioether linkage. The dPEG® spacer in this product increases the hydrophilicity of the conjugate.

This product has proven useful in a variety of applications that take advantage of the high streptavidin-biotin binding affinity. Such applications include magnetic bead-based assays, assays to quantitate reducible thiols in proteins, atomic force microscopy, and nanoparticle applications, among others.

To use Biotin-dPEG®11-MAL, the product should be dissolved in dry solvent and then added to an aqueous solution of the compound containing the free thiol. The water-miscible solvent N,N’-dimethylacetamide (DMAC) dried over 3Å molecular sieves is an excellent solvent to use for this purpose. For biomolecules (e.g., antibodies), care should be taken to keep the amount of organic solvent to a minimum so as not to denature the biomolecule or cause salt precipitation.

We also sell two related products with longer dPEG® spacers between the biotin and maleimide moieties. These products are PN10201, Biotin-dPEG®3-MAL, and PN10785, Biotin-dPEG®23-MAL. If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Application References:

  1. Hermanson, G. T. Chapter 11, (Strept)avidin-Biotin Systems. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 465-505. Click here for a review of Greg’s book and a link to purchase it.
  2. Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 787-838.

Description

 

 

 

Additional information

Weight.5 oz
Dimensions.75 × .75 × 2 in

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