Specs:

 SDS

Molecular Weight: 491.60; single compound

CAS: 721431-18-1

Purity: > 98%

Spacers: dPEG® Spacer is 16 atoms and 19.2 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Macrocycles That Inhibit the Binding between Heat Shock Protein 90 and TPR-Containing Proteins. Veronica C. Ardi, Leslie D. Alexander, Victoria A. Johnson, and Shelli R. McAlpine. ACS Chem. Biol. 2011, 6 (12), pp 1357–1366. September 27, 2011. DOI: 10.1021/cb200203m.

Sensitive Detection of Small Molecule–Protein Interactions on a Metal – Insulator – Metal Label-Free Biosensing Platform. Amir Syahir, Kotaro Kajikawa and Hisakazu Mihara. Chemistry Asian Journal. 2012, 8 (7), pp 1867-1874. August 2012. DOI: 10.1002/asia.201200138.

Surface Functionalization Using Catalyst-Free Azide-Alkyne Cycloaddition. Alexander Kuzmin, Andrei Poloukhtine, Margreet A. Wolfert, and Vladimir V. Popik. Bioconjugate Chem. 2010, 21 (11) pp 2076–2085. October 21, 2010. DOI: 10.1021/bc100306u.


Measurement of Inflammatory Cytokine Secretion from Human Monocytes after Inflammasome Activation. Yoshitaka Shirasaki, Asahi Nakahara, Nanako Shimura, Kazushi Izawa, Nobutake Suzuki, Mai Yamagishi, Jun Mizuno, Tetsushi Sekiguchi, Ryuta Nishikomori, Shuichi Shoji, Osamu Ohara. Royal Society of Chemistry. 2012, 978-0-9798064-5-2 pp 1012-1014. November 1, 2012. DOI: 12CBMS-0001.

Prion protein 90-231 contains a streptavidin-binding motif. Thurid Boetel, Steffen Bade, Marcus Alexander Schmidt, Andreas Frey. Biochemical and Biophysical Research Communications. 2006, 349 (1) pp 296-302. October 13, 2006. DOI: 10.1016/j.bbrc.2006.08.041.

Real-time single-cell imaging of protein secretion. Yoshitaka Shirasaki, Mai Yamagishi, Nobutake Suzuki, Kazushi Izawa, Asahi Nakahara, Jun Mizuno, Shuichi Shoji, Toshio Heike, Yoshie Harada, Ryuta Nishikomori and Osamu Ohara. Scientific Reports. 2014, 4 (4736) pp 1-16. April 22, 2014. DOI: 10.1038/srep04736.

Synthesis of Three-dimensional Polymer Nanostructures via Chemical Vapor Deposition. Kenneth Chang. Dissertations and Theses (Ph.D. and Master's). 2017, pp iv-157. 10/5/2017.

A high-resolution real-time quantification of astrocyte cytokine secretion under shear stress for investigating hydrocephalus shunt failure. Fatemeh Khodadadei, Allen P. Liu & Carolyn A. Harris. Communications Biology. 2021. 4, Article number: 387 (2021). 03/23/2021. DOI: 10.1038/s42003-021-01888-7

Quantitative live-cell imaging of secretion activity reveals dynamic immune responses. Mai Yamagishi, Kaede Miyata, Takashi Kamatani, Hiroki Kabata, Rie Baba, Yumiko Tanaka, Nobutake Suzuki, Masako Matsusaka, Yasutaka Motomura, Tsuyoshi Kiniwa, Satoshi Koga, Keisuke Goda, Osamu Ohara, Takashi Funatsu, Koichi Fukunaga, Kazuyo Moro, Sotaro Uemura, Yoshitaka Shirasaki. bioRxiv. 2022. January 10, 2022. https://doi.org/10.1101/2022.02.09.479547

Additional Search Phrases:

dPEG4-biotin acid, 721431181, PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, biotin, biotin label, biotin labeling, biotin avidin, biotin streptavidin, amine reactive, biotinylation, PEG4, Biotin-PEG4-acid, Biotin-PEG4-COOH, PEG4-biotin acid, Carboxy-PEG4-biotin



 SDS    Datasheet

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dPEG®₄-biotin acid

$100.00 – $600.00

Clear
PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses
Product#: 10199 Categories: , ,

Product number 10199, dPEG®4 biotin acid, is the precursor product of our highly popular PN10200, NHS-dPEG®4-biotin, and our increasingly popular PN10009, Biotin-dPEG®4-TFP ester. This product is a free acid rather than an active ester and can be functionalized for a variety of uses.

Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or to a surface such as glass or gold. It is widely used in bioconjugation research and product development. Biotin is normally poorly soluble in water, but the amphiphilic dPEG® linker imparts excellent solubility in water or aqueous buffer and in organic solvent. In the past, many experiments have coupled aminocaproic acid to biotin to use as a spacer between biotin and the surface to be modified. Unfortunately, aminocaproic acid (often abbreviated as LC, for “long chain”) is highly hydrophobic. Insoluble in water, LC-biotin must be dissolved in an organic solvent such as DMF or DMSO before using in aqueous buffer. When conjugated to proteins or other biomacromolecules, LC-biotin can trigger aggregation and precipitation of conjugated molecules due to hydrophobic interactions. In assays, the hydrophobicity of LC-biotin also increases background noise through non-specific binding.

Amphiphilic dPEG®4 biotin acid can be dissolved both in water or aqueous buffer and in organic solvent. It does not cause aggregation or precipitation of biomolecules. Using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry, PN10199 can be coupled directly to free primary amines in aqueous media. Moreover, the acid moiety can be functionalized with other reactive groups for coupling to surfaces such as glass, gold, or magnetic nanoparticles. The linker length of dPEG®4 biotin acid is only slightly longer than the linker length of LC-biotin, but dPEG®4 biotin acid has far superior performance characteristics compared to LC-biotin.

This product has been used in a variety of applications that take advantage of the strong avidin-biotin binding interaction. Such applications include single cell imaging of protein secretion, developing biosensors, measuring cytokine response during inflammation, and assembling supramolecular nanostructures.

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Application References:

  1. Hermanson, G. T. Chapter 11, (Strept)avidin-Biotin Systems. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 465-505. Click here for a review of Greg’s book and a link to purchase it.
  2. Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 787-838.

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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