Specs:

 SDS

Molecular Weight: 588.67; single compound

CAS: 459426-22-3

Spacers: dPEG® Spacer is 16 atoms and 19.2 Å

References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-730 in his chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

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Bioconjugation of Ln3+-Doped LaF3 Nanoparticles to Avidin. Peter R. Diamente, Robert D. Burke, and Frank C. J. M. van Veggel. Langmuir. 2006, 22 (4) pp 1782–1788. December 30, 2005. DOI: 10.1021/la052589r.


Psoralen Conjugates for Visualization of Genomic Interstrand Cross-Links Localized by Laser Photoactivation. Arun Kalliat Thazhathveetil, Su-Ting Liu, Fred E. Indig, and Michael M. Seidman. Bioconjugate Chemistry. 2007, 18 (2) pp 431–437. January 9, 2007. DOI: 10.1021/bc060309t.

Entwicklung von Antikörper-Mikroarray: von Biophysik der Mikrospot-Reaktion bis zur Hochdurchsatzanalyse der Proteine. Vorgelegt von Wals Kusnezow, Nishnij, Ural and Russische Foderation. Proteomics 2005, 6 pp 1-285 June 27, 2005. DOI: 10.1002/pmic.200500149.

A novel immunotherapy for superficial bladder cancer by intravesical immobilization of GM-CSF. Zhiming Hu, Wanlong Tan, Lin Zhang, Zhongkun Liang, Cuixiang Xu , Hua Su, Jianxin Lu, Jimin Gao. Journal of Cellular and Molecular Medicine. 2010, 14 (6b) pp 1836-1844. July 20, 2009. DOI: 10.1111/j.1582-4934.2009.00818.x.

A novel method for quantitative measurement of a therapeutic monoclonal antibody in the presence of its target protein using enzymatic digestion. Julie Doucet, Alexandre Avrameas. Journal of Pharmaceutical and Biomedical Analysis. 2010, 52 (4) pp 565–570. August 1, 2010. DOI:10.1016/j.jpba.2010.01.033.

A single step multiplex immunofluorometric assay for differential diagnosis
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Bioengineered surfaces to improve the blood compatibility of biomaterials through direct thrombin inactivation. S.C. Freitas, T.B. Cereija, A.C. Figueiredo, H. Osório P.J.B. Pereira, M.A. Barbosa, M.C.L. Martins. Acta Biomaterialia. 2012, 8 (11) pp 4101–4110. November 2012. DOI:10.1016/j.actbio.2012.07.020.

Increased cytochrome c in rat cerebrospinal fluid after cardiac arrest and its effects on hypoxic neuronal survival. Hao Liua, Syana M. Sarnaikc, Mioara D. Manolec, Yaming Chend, Sunita N. Shindeb, Wenjin Li, Marie Rosea, Henry Alexandere, Jie Chenb, Robert S.B. Clarkd, Steven H. Grahama, Robert W. Hickey. Resuscitation. 2012, 83 (12) pp 1491– 1496. December 2012. DOI: 10.1016/j.resuscitation.2012.04.009.

Prion protein detection in serum using micromechanical resonator arrays. Madhukar Varshneya, Philip S.Waggonera, Richard A. Montagnab, Harold G. Craighead. Talanta. 2009, 80 (2) pp 593–599. December 15, 2009. DOI:10.1016/j.talanta.2009.07.032.

Relevant antibody subsets against MOG recognize conformational epitopes exclusively exposed in solid-phase ELISA. Til Menge, Hans-Christian von Budingen Patrice H Lalive, Claude P Genain. European Journal of Immunology. 2007, 37 (11) pp 3229-3239. October 4, 2007. DOI: 10.1002/eji.200737249.

Synthetic, site-specific biotinylated analogs of human MCP-1. Marian Kruszynski,* Ping Tsui, Nicole Stowell, Jinquan Luo, Jennifer F. Nemeth, Anuk M. Das, Raymond Sweet, George A. Heavner. Journal of Peptide Science. 2005, 12 (5) pp 354-360. November 14, 2005. DOI: 10.1002/psc.734.

Urinary kidney injury molecule -1: a sensitive quantitative biomarkers for early detection of kidney tubular injury. Vishal S. Vaidya, Victoria Ramirez, Takaharu Ichimura, Norma A. Bobadilla and Joseph V. Bonventre. American Journal of Physiology/Renal Physiology. 2006, 290 (2) pp F517-F529. February 1, 2006. DOI: 10.1152/ajprenal.00291.2005.

Validation of serum protein profiles by a dual antibody array approach. Rebecca Rimini, Jochen M. Schwenk, Mårten Sundberg, Ronald Sjöberg, Daniel Klevebring, Marcus Gry, Mathias Uhlén, Peter Nilsson. Journal of Proteomics. 2009, 73 (2) pp 252-266. December 1, 2009. DOI: 10.1016/j.jprot.2009.09.009.

Weak Adsorption-Induced Surface Stress for Streptavidin Binding to Biotin Tethered to Silicon Microcantilever Arrays. Stanley J. Ness, Ryan R. Anderson, Weisheng Hu, Danny C. Richards, Joseph Oxborrow, Timothy Gustafson,Ben Tsai, Seunghyun Kim, Brian Mazzeo, Adam Woolley, Gregory P. Nordin. IEEE Sensors Journal. 2013, 13 (3) pp 959-968. October 18, 2012. DOI:10.1109/JSEN.2012.2225613.

Thyroid-stimulating hormone (TSH): Measurement of intracellular, secreted, and circulating hormone in Xenopus laevis and Xenopus tropicalis. Joseph J. Korte, Robin M. Sternberg, Jose A. Serrano, Kara R. Thoemke, Scott M. Moen, Kathryn E. Lillegard, Michael W. Hornung, Joseph E. Tietge, Sigmund J. Degitz. General and Comparative Endocrinology. 2011, 171 (3) pp 319-325. May 1, 2011. DOI: 10.1016/j.ygcen.2011.02.017.

Synthesis, characterization and applications of tether supported biomembrane-microsphere assemblies. Bin He, M. Lane Gilchrist. IEEE Xplore. 2010, pp 1-2. March 28, 2010. DOI: 10.1109/NEBC.2010.5458221.

Subnanometre single-molecule localization, registration and distance measurements. Alexandros Pertsinidis, Yunxiang Zhang, Steven Chu. Nature. 2010, 466 pp 647-651. July 29, 2010. DOI: 10.1038/nature09163.

Solution Structural Dynamics of HIV-1 Reverse Transcriptase Heterodimer. James M. Seckler, Kathryn J. Howard, Mary D. Barkley, Patrick L. Wintrode. Biochemistry. 2009, 48 (32) pp7646-7655. July 13, 2009. DOI: 10.1021/bi900790x.

Solid-phase biotinylation of antibodies. Elizabeth Strachan, A. Krishna Mallia, Joanna M. Cox, Babu Antharavally, Surbhi Desai, Laura Sykaluk, Valerie O’Sullivan, Peter A. Bell. Journal of Molecular Recognition. 2004, 17 (3) pp 268-276. April 27, 2004. DOI:10.1002/jmr.669.

Rapid prediction of expression and refolding yields using phage display. Kip Dudgeon, Romain Rouet, Daniel Christ. Protein Engineering Design & Selection. 2013, 26 (10) pp 671-674. April 13, 2013. DOI: 10.1093/protein/gzt019.

PG27 is a novel membrane protein essential for a Porphyromonas gingivalis protease secretion system. Ikumi Ishiguro, Keitarou Saiki, Kiyoshi Konishi. FEMS Microbiology Letters. 2009, 292 (2) pp 261-267. March 1, 2009. DOI:10.1111/j.1574-6968.2009.01489.x.

Optical glutamate sensor for spatiotemporal analysis of synaptic transmission. Shigeyuki Namiki, Hirokazu Sakamoto, Sho Iinuma, Masamitsu Iino, Kenzo Hirose. European Journal of Neuroscience. 2007, 25 (8) pp 2249-2259. April 16, 2007. DOI: 10.1111/j.1460-9568.2007.05511.x.

Monolithic columns with immobilized monomeric avidin: preparation and application for affinity chromatography. Jens Sproß, Andrea Sinz. Analytical and Bioanalytical Chemistry. 2012, 402 (7) pp 2395-2405. January 20, 2012. DOI: 10.1007/s00216-011-5670-3.

Microstructured layers of spherical biofunctional core-shell nanoparticles provide enlarged reactive surfaces for protein microarrays. Kirsten Borchers, Achim Weber, Herwig Brunner, Gunter E. M. Tovar. Analytical and Bioanalytical Chemistry. 2005, 383 (5) pp 738-746. August 11, 2005. DOI: 10.1007/s00216-005-3396-9.

Large-Scale Identification of Bacteria-Host Crosstalk by Affinity Chromatography: Capturing the Interactions of Streptococcus suis Proteins with Host Cells. Bo Chen, Anding Zhang, Zhongmin Xu, Ran Li, Huanchun Chen, Meilin Jin. Journal of Proteome Research. 2011, 10 (11) pp 5163-5174. September 26, 2011. DOI: org/10.1021/pr200758q.

Mapping the Membrane Topology and Extracellular Ligand Binding Domains of the Retinol Binding Protein Receptor. Riki Kawaguchi, Jiamei Yu, Patrick Wiita, Mariam Ter-Stepanian, Hui Sun. Biochemistry. 2008, 47 (19) pp 5387-5395. April 18, 2008. DOI: 10.1021/bi8002082.

Fibronectin Type III Domain Based Monobody with High Avidity. Jinzhu Duan, Jinsong Wu, C. Alexander Valencia, Rihe Liu. Biochemistry. 2007, 46 (44) pp 12656-12664. October 12, 2007. DOI: 10.1021/bi701215e.

Detection of botulinum neurotoxins in buffer and honey using a surface plasmon resonance (SPR) sensor. Jon Ladd, Allen D. Taylor, Jiri Homola, Shaoyi Jiang. Sensors and Actuators B: Chemical. 2008, 130 (1) pp 129-134. March 14, 2008. DOI: 10.1016/j.snb.2007.07.140.

Comparative protein profiling of serum and plasma using an antibody suspension bead array approach. Jochen M. Schwenk, Ulrika Igel, Bernet S. Kato, George Nicholson, Fredrik Karpe, Mathias Uhlen, Peter Nilsson. Proteomics. 2010, 10 (3) pp 532-540. December 1, 2009. DOI : 10.1002/pmic.200900657.

Classification of protein profiles from antibody microarrays using heat and detergent treatment. Anna Haggmark, Maja Neiman, Kimi Drobin, Martin Zwahlen, Mathias Uhlen, Peter Nilsson, Jochen M. Schwenk. New Biotechnology. 2012, 29 (5) pp 564-570. June 15, 2012. DOI: 10.1016/j.nbt.2011.10.005.

Chemical and biological characterisation of a sensor surface for bioprocess monitoring. Jonathan D. Moore, Miguel A. Perez-Pardo, Jonathan F. Popplewell, Steve J. Spencer, Santanu Ray, Marcus J. Swann, Alex G. Shard, Walis Jones, Anna Hills, Daniel G. Bracewell. Biosensors and Bioelectronics. 2011, 26 (6) pp 2940-2947. February 15, 2011. DOI: 10.1016/j.bios.2010.11.043.

Characterization of a mouse amelogenin [A−4]/M59 cell surface receptor. Kevin Tompkins, Anne George, Arthur Veis, Kevin Tompkins, Anne George, Arthur Veis. Bone. 2006, 38 (2) pp 172-180. February 2006. DOI: 10.1016/j.bone.2005.08.013.

Calcium-dependent self-association of the C-type lectin domain of versican. Andreas Ney, Patrick Booms, Guido Epple, Matthias Morgelin, Gao Guo, Gerhard Kettelgerdes, Reinhard Geßner, Peter N. Robinson. The International Journal of Biochemistry & Cell Biology. 2006, 38 ( 1) pp23-29. January 2006. DOI: 10.1016/j.biocel.2005.07.007.

Bifunctional phage-based pretargeted imaging of human prostate carcinoma. Jessica R. Newton-Northup, Said D. Figueroa, Thomas P. Quinn, Susan L. Deutscher. Nuclear Medicine and Biology. 2009, 36 (7) pp 789-800. October 2009. DOI: 10.1016/j.nucmedbio.2009.04.010.

Antibody Suspension Bead Arrays within Serum Proteomics. Jochen M. Schwenk, Marcus Gry, Rebecca Rimini, Mathias Uhlén, Peter Nilsson. Journal of Proteome. 2008, 7 (8) pp 3168-3179. June 28, 2008. DOI: 10.1021/pr700890b.

Antibody Array Analysis with Label-based Detection and Resolution of Protein Size. Weiwei Wu, Heidi Slåstad, Daniel de la Rosa Carrillo, Tom Frey, Geir Tjønnfjord, Eva Boretti, Hans-Christian Aasheim, Vaclav Horejsi, Fridtjof Lund-Johansen. Molecular and Cellular Proteomics. 2008, 8 pp 245-257. September 16, 2008. DOI:10.1074/mcp.M800171-MCP200.

An Anti-Insulin-like Growth Factor I Receptor Antibody That is a Potent Inhibitor of Cancer Cell Proliferation. Erin K. Maloney, Jennifer L. McLaughlin, Nancy E. Dagdigian. Cancer Research. 2003, 63 pp 5073-5083. August 15, 2003. DOI: www.cancerres.aacrjounrals.org/content/63/5073.

Anti-cytokine autoantibodies are ubiquitous in healthy individuals. Masato Watanabea, Kanji Uchidac, Kazuhide Nakagakid, Hiroko Kanazawaa, Bruce C. Trapnellc, Yoshihiko Hoshinoe, Hiroshi Kagamua, Hirohisa Yoshizawaa, Naoto Keichof, Hajime Gotob, Koh Nakata. FEBS Letters. 2007, 581 (10) pp 2017-2021. May 15, 2007. DOI: 10.1016/j.febslet.2007.04.029.

Binding and signaling of surface-immobilized reagentless fluorescent biosensors derived from periplasmic binding proteins. Robert M De Lorimier, Yaji Tian, Homme W. Hellinga. Protein Science. 2006, 15 (8) pp 1936-1944. May 18, 2006. DOI: 10.1110/ps.062261606.

Accurate Quantification of Cardiovascular Biomarkers in Serum Using Protein Standard Absolute Quantification and Selected Reaction Monitoring. Celine Huillet, Annie Adrait, Dorothee Lebert, Guillaume Picard, Mathieu Trauchessec, Mathilde Louwagie, Alain Dupuis, Luc Hittinger, Bijan Ghaleh, Philippe Le Corvoisier, Michel Jaquinod, Je rome Garin, Christophe Bruley, Virginie Brun. Molecular and Celluar Proteomics. 2012, 11 (12) pp 1-11. November 11, 2011. DOI: 10.1074/mcp.M111.008235.

Antibody affinity maturation in vitro using unconjugated peptide antigen. Hiroto Iwai, Bengu Ozturk, Masaki Ihara, Hiroshi Ueda. Protein Engineering Design & Selection. 2010, 23 (4) pp 185-193. February 1, 2010. DOI: 10.1093/protein/gzp093.

A generalized kinetic model for amine modification of proteins with application to phage display. Xiaofang Jin, Jessica Rose Newton, Stephen Montgomery-Smith, George P. Smith. Bio Techniques. 2009, 46 (3) pp 175-182. March 1, 2009. DOI: 10.2144/000113074.

Chemotactic Activity of S100A7 (Psoriasin) Is Mediated by the Receptor for Advanced Glycation End Products and Potentiates Inflammation with Highly Homologous but Functionally Distinct S100A15. Ronald Wolf, O. M. Zack Howard, Hui-Fang Dong, Christopher Voscopoulos, Karen Boeshans, Jason Winston, Rao Divi, Michele Gunsior, Paul Goldsmith, Bijan Ahvazi, Triantafyllos Chavakis, Joost J. Oppenheim, Stuart H. Yuspa. Journal of Immunology. 2008, 181 (2) pp 1499-1506. July 15, 2008. DOI: 10.4049/jimmunol.181.21499

Continuous association of cadherin with b-catenin requires the non-receptor tyrosine-kinase Fer. Gang Xu, Andrew W. B. Craig, Peter Greer, Matthew Miller, Panos Z. Anastasiadis, Jack Lilien, Janne Balsamo. Journal of Cell Science. 2004, 117 (15) pp 3207-3219. July 1, 2004. DOI: 10.1242/jcs.01174.

Modified Tobacco mosaic virus particles as scaffolds for display of protein antigens for vaccine applications. Mark L. Smith, John A. Lindbo, Stephan Dillard-Telm, Paul M. Brosio, Amanda B. Lasnik, Alison A. McCormick, Long V. Nguyen, Kenneth E. Palmer. Virology. 2006, 348 (2) pp 475-488. May 10, 2006. DOI: 10.1016/j.virol.2005.12.039.

Mapping of Conformational IgE Epitopes with Peptide-Specific Monoclonal Antibodies Reveals Simultaneous Binding of Different IgE Antibodies to a Surface Patch on the Major Birch Pollen Allergen, Bet v 1. Anna Gieras, Petra Cejka, Katharina Blatt, Margarete Focke-Tejkl, Birgit Linhart, Sabine Flicker, Angelika Stoecklinger, Katharina Marth, Anja Drescher, Josef Thalhamer, Peter Valent, Otto Majdic, Rudolf Valenta. Journal of Immunology. 2011, 186 (9) pp 5333-5344. March 30, 2011. DOI: 10.4049/jimmunol.1000804.

M Cell–Targeted Ocular Immunization: Effect on Immunoglobulins in Tears, Feces, and Serum. Thomas E. Phillips, Jeremy Sharp, Kay Rodgers, and Hongshan Liu. IVOS. 2010, 51 (3) pp 1533-1539. March 1, 2010. DOI:10.1167/iovs.09-4491.

Integrin Binds to the RGD Motif of Glycoprotein B of Kaposi’s Sarcoma-Associated Herpesvirus and Functions as an RGD-Dependent Entry Receptor. H. Jacques Garrigues, Yelena E. Rubinchikova, C. Michael DiPersio, Timothy M. Rose. Journal of Virology. 2008, 82 (3) pp 1570-1580. November 28, 2007. DOI: 10.1128/JVI.01673-07.

Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite™ antibodies. Comparison with the free light chain oligoclonal pattern. David Zeman, Pavel Hradílek, Zdeněk Švagera, Eva Mojžíšková, Ivana Woznicová, Olga Zapletalová. Fluids and Barriers of the CNS. 2012, 9 (5) pp 1-11. February 23, 2013. DOI: 10.1186/2045-8118-9-5.

Comparison between Ovalbumin and Ovalbumin Peptide 323-339 Responses in Allergic Mice: Humoral and Cellular Aspects. L.-Z. Sun, S. Elsayed, T. B. Aasen, T. Van Do, N. P. Aardal, E. Florvaag, K. Vaali. J. of Immun. 2010, 71 (5) pp 323-339. January 21, 2010. DOI: 10.1111/j.1365-3083.2010.02382.x.

Synthesis and evaluation of cell-permeable biotinylated PU-H71 derivatives as tumor Hsp90 probes. Tony Taldone, Anna Rodina, Erica M. DaGama Gomes, Matthew Riolo, Hardik J. Patel, Raul Alonso-Sabadell, Danuta Zatorska, Maulik R. Patel, Sarah Kishinevsky and Gabriela Chiosis. Beilstein Journal of Organic Chemistry. 2013, 9 pp 544-556. March 15, 2013. DOI: 10.3762/bjoc.9.60.

Connexin 47 (Cx47)-Deficient Mice with Enhanced Green Fluorescent Protein Reporter Gene Reveal Predominant Oligodendrocytic Expression of Cx47 and Display Vacuolized Myelin in the CNS. Benjamin Odermatt, Kerstin Wellershaus, Anke Wallraff, Gerald Seifert, Joachim Degen, Carsten Euwens, Babette Fuss, Heinrich Bu¨ssow, Karl Schilling, Christian Steinha, and Klaus Willecke. The Journal of Neuroscience. 2003, 23(11) pp.4549–4559. June 1, 2003.

Characterization of Notch1 Antibodies That Inhibit Signaling of Both Normal and Mutated Notch1 Receptors. Miguel Aste-Amezaga, Ningyan Zhang, Janet E. Lineberger, Beth A. Arnold, Timothy J. Toner,Mingcheng Gu, Lingyi Huang, Salvatore Vitelli, Kim T. Vo, Peter Haytko, Jing Zhang Zhao, Frederic Baleydier, Sarah L’Heureux, Hongfang Wang, Wendy R. Gordon, Elizabeth Thoryk, Marie Blanke Andrawes, Kittichoat Tiyanont, Kimberly Stegmaier, Giovanni Roti, Kenneth N. Ross, Laura L. Franlin, Hui Wang, Fubao Wang, Michael Chastain, Andrew J. Bett, Laurent P. Audoly, Jon C. Aster, Stephen C. Blacklow, Hans E. Huber. PLoS ONE. 2010, 5(2):e9094.February 8,2010. DOI: 10.1371/journal.pone.0009094.

Phage Display Approaches for the Isolation of Monoclonal Antibodies Against Dengue Virus Envelope Domain III from Human and Mouse Derived Libraries. Nicole J. Moreland , Patricia Susanto , Elfin Lim , Moon Y. F. Tay , Ravikumar Rajamanonmani , Brendon J. Hanson and Subhash G. Vasudevan. International Journal of Molecular Sciences. 2012, 13 (3) pp 2618-2635. February 27, 2012. DOI: 10.3390/ijms13032618.

Optimization of solid phase PLA with emphasis on antibody modification. Junhong Yan. 2010

Biomolecular Strategies for Cell Surface Engineering. John Tanner Wilson. Doctoral Dissertation, Georgia Institute of Technology: Atlanta, Georgia USA, 2009.

Native Polycystin 2 Functions as a Plasma Channel in Renal Epithelia Membrane Ca 2+-Permeable Cation Channel in Renal Epithelia.Ying Luo, Peter M. Vassilev, Xiaogang Li, Yoshifumi Kawanabe and Jing Zhou. Molecular and Cellular Biology. 2003, 23(7) pp. 2600-2607. April 1, 2003. DOI:10.1128/MCB.23.7.2600-2607.

Molecular Cloning, Functional Expression, and Tissue Distribution of a Novel Human Gap Junction-forming Protein, Connexin-31.9: Interaction With Zona Occludens Protein-1. Peter A. Nielsen, Derek L. Beahm, Ben N. G. Giepmans, Amos Baruch, James E. Hall and Nalin M Kumar. The Journal of Biological Chemistry. 2002, 277 (41) pp. 38272-38283. August 1, 2002. DOI: 10.1074/jbc.M205348200.

An analysis of the function and expression of D6 on lymphatic endothelial cells. Clive S. McKimmie, Mark D. Singh, Kay Hewit, Oscar Lopez-Franco, Michelle Le Brocq, Stefan Rose-John, Kit Ming Lee, Andrew H. Baker, Rachel Wheat, David J. Blackbourn, Robert J. B. Nibbs and Gerard J. Graham. Blood Journal. 2013, 121 (18), pp 3768-3777. May 2, 2013. DOI: 10.1182/blood-2012-04-425314.

Induction of Terminal Differentiation in Melanoma Cells on Downregulation of b-Amyloid Precursor Protein. Michelle G. Botelho, Xiaolei Wang, Donna J. Arndt-Jovin, Dorothea Becker and Thomas M. Jovin. Journal of Investigative Dermatology. 2010, 130, pp 1400-1410. September 17, 2009. DOI:10.1038/jid.2009.296.

Ligand-regulated oligomerization of b2-adrenoceptors in a model lipid bilayer. Juan Jose´ Fung, Xavier Deupi, Leonardo Pardo, Xiao Jie Yao, Gisselle A Velez-Ruiz, Brian T DeVree, Roger K Sunahara and Brian K Kobilka. The EMBO Journal. 2009, 28 (21) pp 3315–3328. September 17, 2009. DOI: 10.1038/emboj.2009.267.

SURFIN is a polymorphic antigen expressed on Plasmodium falciparum merozoites and infected erythrocytes. Gerhard Winter, Satoru Kawai, Malin Haeggström, Osamu Kaneko, Anne von Euler, Shin-ichiro Kawazu, Daniel Palm, Victor Fernandez, and Mats Wahlgren. JEM. 2005, 201 (11) pp 1853–1863. June 6, 2005. DOI:10.1084/jem.20041392.

Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter. Soshana Svendsen, Chad Zimprich, Mark G McDougall, Dieter H Klaubert and Georgyi V Los. BMC Cell Biology. 2008, 9 (17) pp 1471. April 2, 2008. DOI: 10.1186/1471-2121-9-17.

Soluble monomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor. J Wykosky, E Palma, D M Gibo, S Ringler, CP Turner and W Debinski. Oncogene. 2008. 27, pp 7260–7273.September 15, 2008. DOI: 10.1038/onc.2008.328.

Recurrence of Intracranial Tumors following Adoptive T Cell Therapy Can Be Prevented by Direct and Indirect Killing Aided by High Levels of Tumor Antigen Cross-Presented on Stromal Cells. Diana L. Thomas, Miri Kim, Natalie A. Bowerman, Samanthi Narayanan, David M. Kranz, Hans Schreiber and Edward J. Roy. The Journal of Immunology. 2009, 183 (3) pp 1828-1837. July 10, 2009. DOI: 10.4049/jimmunol.0802322.

Ferritin Blocks Inhibitory Effects of Two-Chain High Molecular Weight Kininogen (HKa) on Adhesion and Survival Signaling in Endothelial Cells. Lia Tesfay, Annissa J. Huhn, Heather Hatcher, Frank M. Torti1, Suzy V. Torti. PLoS ONE. 2012, 7 (7): e40030. July 2, 2012. DOI:10.1371/journal.pone.0040030.

Bivalent carbohydrate binding is required or biological activity of CNL, the LacdiNAc (GalNAcb1-4GlcNAc)-specific lectin from basidiomycete Clitocybe nebularis. Jure Pohleven, Miha Renko, Spela Magister, David F. Smith, Markus Kuenzler, Borut Strukelj, Dusan Turk, Janko Kos and Jerica Sabotic. The Journal of Biological Chemistry. 2012, 287 (13) pp 10602-10612. February 1, 2012. DOI:10.1074/jbc.M111.317263.

A Small-molecule Inhibitor, 5′-O-Tritylthymidine, targets FAK and Mdm-2 Interaction, and Blocks Breast and Colon Tumorigenesis in vivo. Vita Golubovskaya, Nadia L. Palma, Min Zheng, Baotran Ho, Andrew Magis, David Ostrov, and William G. Cance. Anticancer Agents Med Chem. 2013, 13 (4) pp 532–545. May 1, 2013.

A small molecule focal adhesion kinase (FAK) inhibitor, targeting Y397 site: 1-(2-hydroxyethyl) -3, 5, 7-triaza-1-azoniatricyclo [3.3.1.13,7]decane; bromide effectively inhibits FAK autophosphorylation activity and decreases cancer cell viability, clonogenicity and tumor growth in vivo. Vita M.Golubovskaya, Sheila Figel, Baotran T.Ho, Christopher P. Johnson, Michael Yemma, Grace Huang, Min Zheng, Carl Nyberg, Andrew Magis,David A.Ostrov, Irwin H.Gelman and William G. Cance. Carcinogenesis. 2012, 33 (5) pp 1004–1013. March 7, 2012. DOI:10.1093/carcin/bgs120.

An RNAi therapeutic targeting Tmprss6 decreases iron overload in Hfe mice and ameliorates anemia and iron overload in murine b -thalassemia intermedia. Paul J. Schmidt, Iva Toudjarska, Anoop K. Sendamarai, Tim Racie, Stuart Milstein, Brian R. Bettencourt, Julia Hettinger, David Bumcrot and Mark D. Fleming. Blood Journal. 2013, 121 (7) pp 1200-1208. February 14, 2013. DOI: 10.1182/blood-2012-09-453977.

Evolutionarily divergent herpesviruses modulate T cell activation by targeting the herpesvirus entry mediator cosignaling pathway. Timothy C. Cheung, Ian R. Humphreys, Karen G. Potter, Paula S. Norris, Heather M. Shumway, Bonnie R. Tran, Ginelle Patterson, Rochelle Jean-Jacques, Miri Yoon, Patricia G. Spear, Kenneth M. Murphy, Nell S. Lurain, Chris A. Benedict, and Carl F. Ware. PNAS. 2005, 102 (37) pp 13218-13223. September 13, 2005. DOI: 10.1073/pnas.0506172102.

Epithelial Cell Retention of Transcriptionally Active, P3HR-1-Derived Heterogeneous Epstein-Barr Virus DNA with Concurrent Loss of Parental Virus. Kazufumi Ikuta, Mingyu Ding, Fangfang Zhang, John W. Sixbey and Rona S. Scott. Journal of Virology. 2011, 85 (15) pp 7634-7643. August 1, 2011. DOI: 10.1128/JVI.00045-11.

Diabetes Insipidus in Mice with a Mutation in Aquaporin-2. David J. Lloyd, Frank Wesley Hall, Lisa M. Tarantino, Nicholas Gekakis. PLoS Genetics. 2005, 1 (2) pp e20. August 19, 2005. DOI:10.1371/journal.pgen.0010020.

Development of an ELISA detecting Tumor Protein 53-Induced Nuclear Protein 1 in serum of prostate cancer patients. Houda Saadi, Marion Seillier, Maria Jose Sandi, Sylvain Peuget , Christine Kellenberger, Gwenaelle Gravi, Nelson J. Dusetti, Juan L. Iovanna, Palma Rocchi, Mohamed Amri, Alice Carrier. Results in Immunology. 2013, 3 pp 51-56. May 13, 2013. DOI: 10.1016/j.rinim.2013.05.002

Cutting Edge: A Monoclonal Antibody Specific for the Programmed Death-1 Homolog Prevents Graft-versus-Host Disease in Mouse Models. Dallas B. Flies, Shengdian Wang, Haiying Xu and Lieping Chen. The Journal of Immunology. 2011, 187 (4) pp 1537-1541. July 18, 2011. doi: 10.4049/jimmunol.1100660.

Members of RTP and REEP Gene Families Influence Functional Bitter Taste Receptor Expression. Maik Behrens, Juliane Bartelt, Claudia Reichling, Marcel Winnig, Christina Kuhn, and Wolfgang Meyerhof. The Journal of Biological Chemistry. 2006, 281 (29) pp 20650–20659. July 21, 2006. DOI:10.1074/jbc.M513637200.

Immune response to a potyvirus with exposed amino groups available for chemical conjugation.
Carlos Alberto Manuel-Cabrera, Ana Márquez-Aguirre, Hernández-Gutiérrez Rodolfo, Pablo César Ortiz-Lazareno, Gabriela Chavez-Calvillo, Mauricio Carrillo-Tripp, Laura Silva-Rosales3 and Abel Gutiérrez-Ortega. Virology Journal. 2012, 9 (75) pp 1-11. March 27, 2012. DOI:10.1186/1743-422X-9-75.

Trafficking of immature F508-CFTR to the plasma membrane and its detection by biotinylation. Yishan Luo, Ken McDonald and John W. Hanrahan. Biochem Journal. 2009, 419 pp 211–219. December 8, 2008. DOI:10.1042/BJ20081869.

Generation of streptavidin-tagged human-granulocyte macrophage colony-stimulating factor fusion proteins. Bai Li, Hu Zhiming, Wang Fei, Xu Xiaoling, Xia Chang, Jin Liqin, Li Jinlong, Gao Jimin. Pub Med. 2012, 32 (10) ) pp 1389-1393. October 1, 2012. DOI: PMID:23076170.

Mitoxantrone Targets the ATP-binding Site of FAK, Binds the FAK Kinase Domain and Decreases FAK, Pyk-2, c-Src, and IGF-1R, In Vitro Kinase Activities. Vita Golubovskaya, Baotran Ho, Min Zheng, Andrew Magis, David Ostrov and William Cance. Anticancer Agents Med Chem. 2013, 13 (4) pp 546-554. May 1, 2013. DOI: PMC3625494.

Single-Molecule Detection for Femtomolar Quantification of Proteins in Heterogeneous Immunoassays. Eric A. Nalefski, Christina M. D’Antoni, Evan P. Ferrell, Janice A. Lloyd, Haoqun Qiu, John L. Harris, and Duncan H. Whitney. Clinical Chemistry. 2006, 52 (11) pp 2172-2175. November 1, 2006. DOI: 10.1373/clinchem.2006.072850.

Micro-total analysis system for virus detection: microfluidic pre-concentration coupled to liposome-based detection. John T. Connelly, Sowmya Kondapalli, Marc Skoupi, John S. Parker, Brian J. Kirby, Antje J. Baeumner. Analytical and Bioanalytical Chemistry. 2012, 402 (1) pp 315-323. September 10, 2011. DOI: 10.1007/s00216-011-5381-9.

Formation of raloxifene homo-dimer in CYP3A4, evidence for multi-substrate binding in a single catalytically competent P450 active site. John A. Davis, Robert J. Greene, Sean Han, Dan A. Rock, Larry C. Wienkers. Archives of Biochemistry and Biophysics. 2011, 513 (2) pp 110-118. September 15, 2011. doi:10.1016/j.abb.2011.06.016.

Comparative study of thiolated protein G scaffolds and signal antibody conjugates in the development of electrochemical immunosensors. Jeremy M. Fowler, Margaret C. Stuart, Danny K.Y. Wong. Europe PubMed Central. 2007, 23 (5) pp 633-639. July 28, 2007. DOI: 10.1016/j.bios.2007.07.007.

Detection of viral bioagents using a shear horizontal surface acoustic wave biosensor. M. Bisoffi, B. Hjelle, D.C. Brown, D.W. Branch, T.L. Edwards, S.M. Brozik, V.S. Bondu-Hawkins, R.S. Larson. Biosensors and Bioelectronics. 2008, 23 (9) pp 1397-1403. April 15, 2008. DOI: 10.1016/j.bios.2007.12.016.

Reduction of matrix interferences by the combination of chaotropic salt and DMSO in a broadly applicable target-based ELISA for pharmacokinetic studies of therapeutic monoclonal antibodies. Julie Doucet , Jasna Canadi, Christoph Kalis, Marie-Anne Valentin, Séverine Marrony ,Fabienne Deckert-Salva, Francois Legay, Alexandre Avrameas. Journal of Pharmaceutical and Biomedical Analysis. 2009, 50 (5) pp 924-931. December 5, 2009. DOI :10.1016/j.jpba.2009.06.029.

Plasma Profiling Reveals Human Fibulin-1 as Candidate Marker for Renal Impairment. Maja Neiman, Jesper J. Hedberg, Pierre R. D€onnes, Ina Schuppe-Koistinen, Stephan Hanschke, Ralf Schindler, Mathias Uhl’en, Jochen M. Schwenk, and Peter Nilsson. Journal of Proteome Research. 2011, 10 (11) pp 4925-4934. September 3, 2011. DOI.org/10.1021/pr200286c.

DNA sequencing by denaturation: experimental proof of concept with an integrated fluidic device. Ying-Ja Chen, Eric E. Roller and Xiaohua Huang. Lab Chip. 2010, 10 (9) pp 1153-1159. May 7, 2010. DOI: 10.1039/b921417h.

Structure and dynamics of single DNA molecules manipulated by magnetic tweezers and or flow. Sanford H. Leuba, Travis B. Wheeler , Chao-Min Cheng , Philip R. LeDuc , Mónica Fernández-Sierra , Edwin Quiñones. Methods. 2009, 47 (3) pp 214-222. November 17, 2008. DOI:10.1016/j.ymeth.2008.10.022.

Three-dimensional organotypic models of human colonic epithelium to study the early stages of enteric salmonellosis. Kerstin Ho¨ner, zu Bentrup,Rajee Ramamurthy,C. Mark Ott , Kamal Emami, Mayra Nelman-Gonzalez , James W. Wilson , Emily G. Richter , Thomas J. Goodwin , J. Stephen Alexander , Duane L. Pierson , Neal Pellis , Kent L. Buchanan , Cheryl A. Nickerson. Microbes and Infection. 2006, 8 (7) pp 1813-1825. April 27, 2006. DOI:10.1016/j.micinf.2006.02.020.

Engineering the binding properties of the T cell receptor:peptide:MHC ternary complex that governs T cell activity. Natalie A. Bowerman, Terence S. Crofts, Lukasz Chlewicki, Priscilla Do, Brian M. Baker, K. Christopher Garcia, David M. Kranz. Molecular Immunology. 2009, 46 (15) pp 3000-3008. June 12, 2009. DOI: 10.1016/j.molimm.2009.06.012.

High-Throughput Screening of Small Molecules Identifies Hepcidin Antagonists. Eileen Fung, Priscilla Sugianto, Jason Hsu, Robert Damoiseaux, Tomas Ganz, and Elizabeta Nemeth. Molecular Pharmacology. 2013, 83 (3) pp 681-690. January 4, 2013. DOI: org/10.1124/mol.112.083428.

Identification of Quantum Dot Bioconjugates and Cellular Protein Co-localization by Hybrid Gel Blotting. Hong Yan Liu and Tania Q. Vu. Nano Letters. 2007, 7 (4) pp 1044-1049. March 3, 2007. DOI: 10.1021/nl070239e.

Multilayer enzyme-coupled magnetic nanoparticles as efficient, reusable biocatalysts and biosensors. Josep Garcia, Yue Zhang, Hannah Taylor, Oscar Cespedes, Michael E. Webb and Dejian Zhou. Nanoscale. 2011, 3 (9) pp 3721-3730. June 10, 2011. DOI: 10.1039/C1NR10411J.

Structure-based design of robust glucose biosensors using a Thermotoga maritima periplasmic glucose-binding protein. Yaji Tian, Matthew J. Cuneo, Anita Changela, Birte Hocker, Lorena S. Beese, and Homme W. Hellinga. Protein Science. 2007, 16 (10) pp 2240-2250. January 1, 2009. DOI: 10.1110/ps.072969407.

Generation of monospecific antibodies based on affinity capture of polyclonal antibodies. Barbara Hjelm, Bjorn Forsstrom, Ulrika Igel, Henrik Johannesson, Charlotte Stadler, Emma Lundberg, Fredrik Ponten, Anna Sjoberg, Johan Rockberg, Jochen M. Schwenk, Peter Nilsson, Christine Johansson, and Mathias Uhlen. Protein Science. 2011, 20 (11) pp 1824-1835. October 12, 2011. DOI: 10.1002/pro.716.

Antibody-based profiling of cerebrospinal fluid within multiple sclerosis. Anna Haggmark, Sanna Bystrom, Burcu Ayoglu, Ulrika Qundos, Mathias Uhlen, Mohsen Khademi, Tomas Olsson, Jochen M. Schwenk and Peter Nilsson. Proteomics, 2013, 13 (15) pp 2256-2267. June 25, 2013. DOI: 10.1002/pmic.201200580.

Selection of Human VH Single Domains with Improved Biophysical Properties by Phage Display. Kip Dudgeon , Romain Rouet , Kristoffer Famm , and Daniel Christ. Methods in Molecular Biology. 2012, 911 pp 383-397. July 12, 2012. DOI: 10.1007/978-1-61779-968-6_23.

Highly sensitive Mach–Zehnder interferometer biosensor based on silicon nitride slot waveguide. Qing Liu, Xiaoguang Tu, Kyung Woo Kim, Jack Sheng Kee, Yong Shin, Kyungsup Han, Yong-Jin Yoon, Guo-Qiang Lo, Mi Kyoung Park. Sensors and Actuators B: Chemical. 2013, 188 pp 681-688. July 25, 2013. DOI: 10.1016/j.snb.2013.07.053.

Neuregulin-1 is neuroprotective in a rat model of organophosphate-induced delayed neuronal injury. Yonggang Li , Pamela J. Lein , Cuimei Liu , Donald A. Bruun , Cecilia Giulivi , Gregory D. Ford , Teclemichael Tewolde , Catherine Ross-Inta , Byron D. Ford. Toxicology and Applied Pharmacology. 2012, 262 (2) pp 194-204. July 15, 2012. DOI: 10.1016/j.taap.2012.05.001.

Detection of human platelet antigen-1a alloantibodies in cases of fetomaternal alloimmune thrombocytopenia using recombinant b3 integrin fragments coupled to fluorescently labeled beads. Winnie Chong, Paul Metcalfe, Rosey Mushens, Geoff Lucas,Willem H. Ouwehand, and Cristina V. Navarrete. Transfusion. 2010, 51 (6) pp 1261-1270. December 16, 2010. DOI: 10.1111/j.1537-2995.2010.02977.x.

A plant derived multifunctional tool for nanobiotechnology based on Tomato bushy stunt virus. Simone Grasso, Chiara Lico, Francesca Imperatori, Luca Santi. Transgenic Research. 2013, 22 (3) pp 519-535. October 30, 2012. DOI:10.1007/s11248-012-9663-6.

An analysis of the function and expression of D6 on lymphatic endothelial cells. Clive S. McKimmie, Mark D. Singh, Kay Hewit, Oscar Lopez-Franco, Michelle Le Brocq, Stefan Rose-John, Kit Ming Lee, Andrew H. Baker, Rachel Wheat, David J. Blackbourn, Robert J. B. Nibbs and Gerard J. Graham. Blood Journal. 2013, 121 pp 3768-3777. March 11, 2013. DOI:10.1182/blood-2012-04-425314.

Light-Up Hoechst–DNA Aptamer Pair: Generation of an Aptamer-Selective Fluorophore from a Conventional DNA-Staining Dye. Shinsuke Sando, Atsushi Narita, and Yasuhiro Aoyama. ChemBioChem. 2007, 8 (15) pp 1795-1803. October 15, 2007. DOI: 10.1002/cbic.200700325.

A Fresnel zone plate biosensor for signal amplification with enhanced signal-to-noise ratiow. Yong-Cheol Jeong, Bokyung Jung, Jung-Hwan Park and Jung-Ki Park. Chemical Communications. 2012, 48 (51) pp 6378-6380. May 2, 2012. DOI: 10.1039/C2CC32008H.

Anti-tumor efficacy of chitosan-g-poly(ethylene glycol) nanocapsules containing docetaxel: Anti-TMEFF-2 functionalized nanocapsules vs. non-functionalized nanocapsules. Daniel Torrecilla, Maria V. Lozano, Enrique Lallana, Jose I. Neissa, Ramon Novoa-Carballal, Anxo Vidal, Eduardo Fernandez-Megia, Dolores Torres, Ricardo Riguera, Maria J. Alonso, Fernando Dominguez. European Journal of Pharmaceutics and Biopharmaceutics. 2013, 83 (3) pp 330-337. April 1, 2013. DOI: 10.1016/j.ejpb.2012.10.017.

Robust dithiocarbamate-anchored amine functionalization of Au nanoparticles. Kai Chen, Hans D. Robinson. Journal of Nanoparticle Research. 2011, 13 (2) pp 751-761. February 1, 2011. DOI: 10.1007/s11051-010-0075-3.

Autocatalytic Activation of Influenza Hemagglutinin. Jeong H. Lee, Mark Goulian, Eric T. Boder. Journal of Molecular Biology. 2006, 364 (3) pp 275-282. December 1, 2006. DOI: 10.1016/j.jmb.2006.09.015.

All major prion types recognised by a multiplex immunofluorometric assay for disease screening and confirmation in sheep. Yue Tang, Adriana Gielbert, Jorg G. Jacobs, Thierry Baron, Olivier Andreoletti, Takashi Yokoyama, Jan P.M. Langeveld, Maurice J. Sauer. Journal of Immunological Methods. 2012, 380 (1-2) pp 30-39. June 29, 2012. DOI: 10.1016/j.jim.2012.03.004.

An extended range generic immunoassay for total human therapeutic antibodies in preclinical pharmacokinetic studies. Colin M. Hall, Josh T. Pearson, Vimal Patel, Larry C. Wienkers, Robert J. Greene. Journal of Immunological Methods. 2013, 393 (1-2) pp 70-73. July 31, 2013. DOI: 10.1016/j.jim.2013.03.011.

Characterization and use of a rabbit-anti-mouse VPAC1 antibody by flow cytometry. Rebecca J. Hermann, Travis Van der Steen, Emilie E. Vomhof-DeKrey, Sejaa Al-Badrani, Steve B. Wanjara, Jarrett J. Failing, Jodie S. Haring, Glenn P. Dorsam. Journal of Immunological Methods. 2012, 376 (1-2) pp 20-31. February 28, 2012. DOI:10.1016/j.jim.2011.10.009.

Possible involvement of glycolipids in lectin-mediated cellular transformation of symbiotic microalgae in corals. Mitsuru Jimbo, Yuya Suda, Kazuhiko Koike, Sachiko Nakamura-Tsuruta, Junko Kominami, Masugu Kamei, Jun Hirabayashi Ryuichi Sakai, Hisao Kamiya. Journal of Experimental Marine Biology and Ecology. 2013, 439 pp 129-135. January 1, 2013. DOI: 10.1016/j.jembe.2012.10.022.


Non-healing is associated with persistent stimulation of the innate immune response in chronic venous leg ulcers. Brita S. Pukstad, Liv Ryan, Trude H. Flo, Jørgen Stenvik, Ryan Moseley, Keith Harding, David W. Thomas, Terje Espevik. Journal of Dermatological Science. 2010, 59 (2) pp 115-122. August 1, 2010. DOI: 10.1016/j.jdermsci.2010.05.003.

G-protein coupled receptor-associated sorting protein 1 (GASP-1), a ubiquitous tumor marker. Xiaoyi Zheng, Frank Chang, Xinmin Zhang, Vicki L. Rothman, George P. Tuszynski. Experimental and Molecular Pathology. 2012, 93 (1) pp 111-115. August 1, 2012. DOI: 10.1016/j.yexmp.2012.03.013.

P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity. Anja W. Fjorback, Sabrina Sundbye, Justus C. Dachsel, Steffen Sinning, Ove Wiborg and Poul H. Jensen. FEBS Journal. 2011, 278 (3) pp 493-505. December 23, 2010. DOI: 10.1111/j.1742-4658.2010.07970.x.

Quantitative analysis of multivesicular bodies (MVBs) in the hypoglossal nerve: Evidence that neurotrophic factors do not use MVBs for retrograde axonal transport. Amy L. Altick, Larisa M. Baryshnikova, Tania Q. Vu and Christopher S. von Bartheld. Journal of Comparative Neurology. 2009, 514 (6) pp 641-657. March 18, 2009. DOI: 10.1002/cne.22047.

Tagging and Enriching Proteins Enables Cell-Specific Proteomics. Thomas S. Elliott, Ambra Bianco, Fiona M. Townsley, Stephen D. Fried, and Jason W. Chin. Cell Chemical Biology. 2016, 23 pp 805-815. July 21, 2016. http://dx.doi.org/10.1016/j.chembiol.2016.05.018.

Thrombospondin-1 Mimetic Agonist Peptides Induce Selective Death in Tumor Cells: Design, Synthesis and Structure-Activity Relationship Studies. Thomas Denefle, Heloise Boullet, Linda Herbi, Clara Newton, Ana-Carolina, Martinez-Torres, Alexandre Guez, Elodie Pramil, Claire Quiney, Marilyne Pourcelot, Mikail Daniel Levasseur, Eva Larde, Roba Moumne, Francois-Xavier Ogi, Pascal Grondin, Helene Merle-Beral, Olivier Lequin, Santos A. Susin, and Philippe Karoyan. Journal of Medicinal Chemistry. 2016, pp 1-33. August 15, 2016. DOI: 10.1021/acs.jmedchem.6b00781.

In vivo biotinylated calpastatin improves the affinity purification of human m-calpain. Hung Huy Nguyen, Alexander N. Volkov, Guy Vandenbussche, Peter Tompa, and Kris Pauwels. Protein Expression and Purification. 2018, 145 pp 77-84. January 13, 2018. DOI: 10.1016/j.pep.2018.01.002.

Evaluation of Antibody Biotinylation Approaches for Enhanced Sensitivity of Single Molecule Array (Simoa) Immunoassays. Limor Cohen and David R. Walt. Bioconjugate Chemistry. 2018, pp A-G and pp1-2. October 1, 2018. DOI: 10.1021/acs.bioconjchem.8b00601.

Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1. Kazuhide Watanabe, Tadahiro Nagaoka, Joseph M. Lee, Caterina Bianco, Monica Gonzales, Nadia P. Castro, Maria Cristina Rangel, Kei Sakamoto, Youping Sun, Robert Callahan, and David S. Salomon. JCB. 2009, 187 (3) pp 343–353. November 2, 2009. DOI: 10.1083/jcb.200905105.

Identification of the human eosinophil lineage-committed progenitor: revision of phenotypic defi nition of the human common myeloid progenitor. Yasuo Mori, Hiromi Iwasaki, Kentaro Kohno, Goichi Yoshimoto, Yoshikane Kikushige, Aki Okeda, Naokuni Uike, Hiroaki Niiro, Katsuto Takenaka, Koji Nagafuji, Toshihiro Miyamoto, Mine Harada, Kiyoshi Takatsu, and Koichi Akashi. The Journal of Experimental Medicine. 2008, 206 (1) pp 183-193. December 29, 2008. DOI: 10.1084/jem.20081756.

AL Amyloid Imaging and Therapy with a Monoclonal Antibody to a Cryptic Epitope on Amyloid Fibrils. Jonathan S. Wall, Stephen J. Kennel, Angela Williams, Tina Richey, Alan Stuckey, Ying Huang, Sallie Macy, Robert Donnell, Robin Barbour, Peter Seubert, Dale Schenk. PLOS ONE. 2012, 7 (12) pp e52686. December 26, 2012. DOI: 10.1371/journal.pone.0052686.

Multidimensional nano-HPLC coupled with tandem mass spectrometry for analyzing biotinylated proteins. Jens Sproß, Sebastian Brauch, Friedrich Mandel, Moritz Wagner, Stephan Buckenmaier, Bernhard Westermann, Andrea Sinz. Analytical and Bioanalytical Chemistry. 2012, 405 (7) pp 2163-2173. May 29, 2012. DOI: 10.1007/s00216-012-6057-9.

Generation and characterization of Anti-AA Amyloid-Specific Monoclonal Antibodies. Jonathan S. Wall , Stephen J. Kennel, Tina Richey, Amy Allen, Alan Stuckey, Deborah T. Weiss, Sallie D. Macy, Robin Barbour, Peter Seubert , Alan Solomon and Dale Schenk. Frontiers in Immunology. 2011, 2 (32). August 8, 2011. DOI: 10.3389/fimmu.2011.00032.

a-Synuclein Senses Lipid Packing Defects and Induces Lateral Expansion of Lipids Leading to Membrane Remodeling. Myriam M. Ouberai, Juan Wang, Marcus J. Swann, Celine Galvagnion, Tim Guilliams, Christopher M. Dobson and Mark E. Welland. The Journal of Biological Chemistry. 2013, June 5, 2013. DOI: 10.1074/jbc.M113.478297.

Site-Specific Labeling of DNA and RNA Using an Efficiently Replicated and Transcribed Class of Unnatural Base Pairs. Young Jun Seo, Denis A. Malyshev, Thomas Lavergne, Phillip Ordoukhanian, and Floyd E. Romesberg . Journal of the American Chemical Society. 2011, 133 (49) pp 19878–19888. October 8, 2011. DOI: 10.1021/ja207907d.

Antibody Repertoire Profiling Using Bacterial Display Identifies Reactivity Signatures of Celiac Disease. Bradley N. Spatola, Joseph A. Murray, Martin Kagnoff, Katri Kaukinen, and Patrick S. Daugherty. Analytical Chemistry. 2013, 85 (2) pp 1215–1222. December 12, 2012. DOI: 10.1021/ac303201d.

Design of a prototype flow microreactor for synthetic biology in vitro. Christian R. Boehm, Paul S. Freemont and Oscar Ces. Lab on a Chip. 2013, 13 (17) pp 3426-3432. June 27, 2013. DOI: 10.1039/c3lc50231g.

A visualized observation of calcium-dependent gelsolin activity upon the surface coverage of fluorescent-tagged actin filaments. Yongkuk Lee, Ming-Yuan Wei, Parviz Famouri. Journal of Colloid and Interface Science. 2013, 389 (1) pp 182–187. January 1, 2013. DOI: 10.1016/j.jcis.2012.08.049.

Selective attachment of F-actin with controlled length for developing an intelligent nanodevice. Ming-Yuan Wei, Lenin J. Leon, Yongkuk Lee, Denzel Parks, Lloyd Carroll, Parviz Famouri. Journal of Colloid and Interface Science. 2011, 356 (1) pp 182-189. April 1, 2011. DOI: 10.1016/j.jcis.2010.12.079.

A pretargeted nanoparticle system for tumor cell labeling. Jonathan Gunn, Steven I. Park, Omid Veiseh, Oliver W. Press and Miqin Zhang. Molecular BioSystems. 2011, 7 (3) pp 742-748. October 13, 2010. DOI: 10.1039/c005154c.

Directional Transport by Nonprocessive Motor Proteins on Fascin-Cross-Linked Actin Arrays. Yongkuk Lee and Parviz Famouri. Nano Letter. 2013, 13 (8) pp 3775–3782. July 2, 2013. DOI: 10.1021/nl401718q.

Layer-by-Layer Assembly of Bioengineered Flagella Protein Nanotubes. Mudalige Thilak Kumara, Brian C Tripp, Subra Muralidharan. Biomacromolecules. 2007, 8 (12) pp 3718-3722. November 03, 2007. DOI: 10.1021/bm7005449.

Preparation of Biotinylated Cypridina Luciferase and Its Use in Bioluminescent Enzyme Immunoassay. Chun Wu, Kosei Kawasaki, Yoko Ogawa, Yasukazu Yoshida, Satoru Ohgiya, and Yoshiro Ohmiya. Analytical Chemistry. 2007, 79 (4) pp 1634-1638. January 13, 2007. DOI: 10.1021/ac061754k.

Prion Protein Detection Using Nanomechanical Resonator Arrays and Secondary Mass Labeling. Madhukar Varshney , Philip S. Waggoner , Christine P. Tan , Keith Aubin , Richard A. Montagna , and Harold G. Craighead. Analytical Chemistry . 2008, 80 (6), pp 2141–2148. February 14, 2008. DOI: 10.1021/ac702153p

Enhancing Peptide Ligand Binding to Vascular Endothelial Growth Factor by Covalent Bond Formation. Bernadette V. Marquez, Heather E. Beck,Tolulope A. Aweda, Brett Phinney, Cynthia Holsclaw, William Jewell, Diana Tran, Jeffrey J. Day, Malalage N. Peiris, Charles Nwosu,Carlito Lebrilla, and Claude F. Meares.Bioconjugate Chemistry. 2012, 23 (5) pp 1080-1089. April 26, 2012. DOI: 10.1021/bc300114d.

Introduction of the Mass Spread Function for Characterization of Protein Conjugates. Joseph P. Skinner, Lianli Chi, Panfilo F. Ozeata,Carol S. Ramsay, Robynn L. O’Hara, Brenda B. Calfin, and Sergey Y. Tetin. Analytical Chemistry. 2012, 84 (2), pp 1172–1177. November 30, 2011. DOI: 10.1021/ac202239j.

Electrochemical Template Synthesis of Multisegment Nanowires:  Fabrication and Protein Functionalization. Bridget Wildt, Prashant Mali, and Peter C. Searson. Langmuir. 2006, 22 (25), pp 10528–10534. September 8, 2006. DOI: 10.1021/la061184j.

Synthetic, site-specific biotinylated analogs of human MCP-1. Marian Kruszynski, Ping Tsui, Nicole Stowell, Jinquan Luo, Jennifer F. Nemeth, Anuk M. Das, Raymond Sweet, George A. Heavner. Journal of Peptide Science. 2006, 12 (5) pp 354-360. November 14, 2005. DOI: 10.1002/psc.734.

Biotinylation reagents for the study of cell surface proteins. Giuliano Elia DR. Proteomics. 2008, 8 (19) pp 4012-4024. September 3, 2008. DOI: 10.1002/pmic.200800097.

Melanoma Imaging with Pretargeted Bivalent Bacteriophage. Jessica R Newton, Yubin Miao, Susan L Deutscher, Thomas P Quinn. Journal of Nuclear Medicine. 2007, 48 (3) pp 429-436. March 1, 2007.

Targeted Delivery of a Photosensitizer to Aggregatibacter actinomycetemcomitans Biofilm. Suci P, Kang S, Gmür R, Douglas T, Young M.. Antimicrobial Agents and Chemotherapy. 2010, 54 (6) pp 2489-2496. April 12, 2010. DOI: 10.1128/AAC.00059-10.

The therapeutic potential of SA-sCD40L in the orthotopic model of superficial bladder cancer. Zhang Z, Xu X, Zhang X, Chen X, Chen Q, Dong L, Hu Z, Li J, Gao J.Acta Oncologica. 2011, 50 (7) pp 1111-1118. October 1, 2011. DOI: 10.3109/0284186X.2010.549838.

Utilising the left-helical conformation of L-DNA for analysing different marker types on a single universal microarray platform. Nicole C. Hauser, Rafael Martinez, Anette Jacob, Steffen Rupp, Jörg D. Hoheisel, and Stefan Matysiak. Nucleic Acids Research. 2006, 34 (18) pp 5101–5111. September 20, 2006. DOI: 10.1093/nar/gkl671.

The Lysosomal Transmembrane Protein 9B Regulates the Activity of Inflammatory Signaling Pathways. Francis Dodeller, Marie Gottar, Dieter Huesken, Vadim Iourgenko and Bruno Cenni. The Journal of Biological Chemistry. 2008, 283 (31) pp 21487-21494. June 9, 2008. DOI: 10.1074/jbc.M801908200.

The Nutrigenetics of Hyperhomocysteinemia. Patricia M. DiBello, Sanjana Dayal, Suma Kaveti, Dongmei Zhang, Michael Kinter, Steven R. Lentz, and Donald W. Jacobsen. Molecular & Cellular Proteomics. 2010, 9 (3) pp 471-485. December 21, 2009. 10.1074/mcp.M900406-MCP200.

Targeted Contrast-Enhanced Ultrasound Imaging of Tumor Angiogenesis with Contrast Microbubbles Conjugated to Integrin-Binding Knottin Peptides. Jürgen K. Willmann, Richard H. Kimura, Nirupama Deshpande, Amelie M. Lutz, Jennifer R. Cochran and Sanjiv S. Gambhir. The Journal of Nuclear Medicine. 2010, 51 (3) pp 433-440. February 11, 2010. DOI: 10.2967/jnumed.109.068007.

Sequence determinants of protein aggregation in human VH domains. Kip Dudgeon, Kristoffer Famm, and Daniel Christ. Protein Engineering, Design & Selection. 2009, 22 (3) pp 217-220. October 28, 2008. DOI: 10.1093/protein/gzn059.

Rapid Tumor Necrosis Factor α-Induced Exocytosis of Glutamate Receptor 2-Lacking AMPA Receptors to Extrasynaptic Plasma Membrane Potentiates Excitotoxicity. Dmitri Leonoudakis, Pingwei Zhao, and Eric C. Beattie. The Journal of Neuroscience. 2008, 28 (9) pp 2119-2130. February 27, 2008. DOI: 10.1523/JNEUROSCI.5159-07.2008.

Polymorphisms of Mouse Apolipoprotein A-II Alter Its Physical and Functional Nature. Timothy J. Sontag, Catherine A. Reardon. PLOS ONE. 2014, 9 (2) e88705. February 10, 2014. DOI:10.1371/journal.pone.0088705.

DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells. Yinghao Zhang, Fang-Mei Chang, Jianjun Huang, Jacob J. Junco, Shivani K. Maffi, Hannah I. Pridgen, Gabriel Catano, Hong Dang, Xiang Ding, Fuquan Yang, Dae Joon Kim,
Thomas J. Slaga, Rongqiao He, Sung-Jen Wei. Protein & Cell. 2013, 5 (2) pp 124-140. February 11, 2014. DOI: 10.1007/s13238-013-0018-8.

Structurally Defined Nanoscale Sheets from Self-Assembly of Collagen-Mimetic Peptides.
Tao Jiang, Chunfu Xu, Yang Liu, Zheng Liu, Joseph S. Wall, Xiaobing Zuo, Tianquan Lian, Khalid Salaita, Chaoying Ni, Darrin Pochan, Vincent P. Conticello. Journal of the American Chemical Society. 2014, 136 (11) pp 4300-4308. February 26, 2014. DOI: 10.1021/ja412867z.

Linker length matters, Fynomer - Fc Fusion with an Optimized Linker Displaying Picomolar IL-17A Inhibition Potency. Michela Silacci, Nadja Baenziger-Tobler, Wibke Lembke, Wenjuan Zha, Sarah Batey, Julian Bertschinger and Dragan Grabulovski. The Journal of Biological Chemistry. 2014, 289 (14) pp 14392-14398. April 1, 2014. DOI: 10.1074/jbc.M113.534578.

Synthetic Antibodies with a Human Framework That Protect Mice from Lethal Sudan Ebolavirus Challenge. Gang Chen, Jayne F. Koellhoffer, Samantha E. Zak, Julia C. Frei, Nina Liu, Hua Long, Wei Ye, Kaajal Nagar, Guohua Pan, Kartik Chandran, John M. Dye, Sachdev S. Sidhu, and Jonathan R. Lai. ACS Chemical Biology. 2014, 9 (10) pp 2263-2273. August 20, 2014. DOI: 10.1021/cb5006454.

New Assay for Quantification of PEGylated Proteins During in Vitro Permeability Studies.
Spela Jalen,, Vanja Smilovic, Katarina Fidler, Barbara Podobnik, Maja Marusic, Radovan Komel, Vladka Gaberc-Porekar and Simona Jevsevar. Acta Chimica Slovenica. 2014, 61 (3) pp 615-622. January 15, 2014.

Calsyntenin3: Molecular Architecture and Interaction with Neurexin 1alpha. Zhuoyang Lu, Yun Wang, Fang Chen, Huimin, Tong, M. V.V.V. Sekhar Reddy, Lin Luo, Suchithra Seshadrinathan, Lei Zhang, Luis, Marcelo F. Holthauzen, Ann Marie Craig, Gang Ren and Gabby Rudenko. Journal of Biological Chemistry. 2014, 289 (50) pp 34530-34542. October 28, 2014. DOI: 10.1074/jbc.M114.606806.

Codon optimization and factorial screening for enhanced soluble expression of human ciliary neurotrophic factor in Escherichia coli. Jaakko M. Itkonen, Arto Urtti, Louise E. Bird, Sanjay Sarkhel. BMC Biotechnology. 2014, 14 (92). November 14, 2014. DOI: 10.1186/s12896-014-0092-x.

Analysis of the sensitizing properties of ragweed pollen componets and the influence of environmental factors in a murine in vivo model. Maria Kamml. Fakultat fur Medizin. 2014, July 5, 2014.

Adapter Protein-1 Complex Affects the Endocytic Trafficking and Function of Peptidylglycine a-Amidating Monooxygenase, a Luminal Cuproenzyme. Mathilde L. Bonnemaison, Nils Back, Megan E. Duffy, Martina Ralle, Richard E. Mains and Betty A. Eipper. Journal of Biological Chemistry. 2015. July 13, 2015. DOI: 10.1074/jbc.M115.641027.

Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality. Janek Kibat, Thomas Schirrmann, Matthias J. Knape, Saskia Helmsing, Doris Meier, Michael Hust, Christoph Schroder, Daniela Bertinetti, Gerhard Winter, Khalid Pardes, Mia Funk, Andrea Vala, Nathalia Giese, Friedrich W. Herberg, Stefan Dubel, and Jorg D. Hoheisel. New Biotechnology. 2015, 00 (00). December 17, 2015. DOI: 101016/j.nbt.2015.12.003.

Antibody humanization by molecular dynamics simulations-in-silico guided selection of critical backmutations. Christian Margreitter, Patrick Mayrhofer, Renate Kunert, and Chris Oostenbrink. Journal of Molecular Recognition. 2016, January 8, 2016. DOI: 10.1002/jmr.2527.

The Tumor Suppressive Small GTPase DiRas1 Binds the Non-canonical Guanine Nucleotide Exchange Factor SmgGDS and Antagonizes SmgGDS Interactions with Oncogenic Small GTPases. Carmen Bergom, Andrew D. Hauser, Amy Rymaszewski, Patrick Gonyo, Jeremy W. Prokop, Benjamin C. Jennings, Alexis J. Lawton, Anne Frei, Ellen L. Lorimer Jr., Irene Aguilera-Barrantes Jr., Alexander C. Mackinnon Jr., Kathleen Noon, Carol A. Fierke, and Carol L. Williams. The Journal of Biological Chemistry. 2016, January 26, 2016. DOI: 10.1074/jbc.M115.696831.

Achieving Potent Autologous Neutralizing Antibody Responses against Tier 2 HIV-1 Viruses by Strategic Selection of Envelope Immunogens. Ann J. Hessell, Delphine C. Malherbe, Franco Pissani, Sean McBurney, Shelly J. Krebs, Michelle Gomes, Shilpi Pandey, William F. Sutton, Benjamin J. Burwitz, Matthew Gray, Harlan Robins, Byung S. Park, Jonah B. Sacha, Celia C. LaBranche, Deborah H. Fuller, David C. Montefiori, Leonidas Stamatatos, D. Noah Sather, and Nancy L. Haigwood. The Journal of Immunology. 2016, pp 1550-6606. March 4, 2016. DOI: 10.4049/jimmunol.1500527.

Nanometric gap structure with fluid lipid bilayer for the selective transport and detection of biological molecules. Koji Ando, Masashi Tanabe, and Kenichi Morigaki. Langmuir. 2016, pp 1-30. July 18, 2016. DOI: 10.1021/acs.langmuir.6b01405.

Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents. Barbara Bernardim, Pedro M.S.D. Cal, Maria J. Matos, Bruno L. Oliveira, Nuria Martinez-Saez, Ines S. Albuquerque, Elizabeth Perkins, Francisco Corzana, Antonio C.B. Burtoloso, Gonzalo Jimenez-Oses, and Goncalo J.L. Bernardes. Nature Communications. 2016, 7 (13128) pp 1-9. October 26, 2016. DOI: 10.1038/ncomms13128.

Surface Plasmon Resonance Imaging Microscopy of Liposomes and Liposome-Encapsulated Gold Nanoparticles. Lauri Viitala, Adam M Maley, H.W. Millie Fung, Robert M. Corn, Tapani Viitala, and Lasse Murtomaki. The Journal of Physical Chemistry. 2016, October 31, 2016. DOI: 10.1021/acs.jpcc.6b09503.

A digital enzyme-linked immunosorbent assay for ultrasensitive measurement of amyloid-ß 1-42 peptide in human plasma with utility for studies of Alzheimer's disease therapeutics. Linan Song, D Richard Lachno, David Hanlon, Adam Shepro, Andreas Jeromin, Dipika Gemani, Jayne A Talbot, Margaret M Racke, Jeffrey L Dage, and Robert A Dean. Alzheimer's Research & Therapy. 2016, 8 (58) pp 1-15. December 15, 2016. DOI: 10.1186/s13195-016-0225-7.

A versatile approach towards nucleobase-modified aptamers. Fabian Tolle, Dr. Gerhard M Brandle, Daniel Matzner, and Prof. Dr. Gunter Mayer. Angewandte Chemie. 2015, 54 (37) pp 10971-10974. September 7, 2015. DOI: 10.1002/anie.201503652.

Transport of biomolecules to binding partners displayed on the surface of microbeads arrayed in traps in a microfluidic cell. Xiaoxiao Chen, Thomas F Leary, and Charles Maldarelli. Biomicrofluidics. 2017, 11 (1) pp 014101. January 4, 2017. DOI: 10.1063/1.4973247.

Affinity purification of human m-calpain through an intrinsically disordered inhibitor, calpastatin. Hung Huy Nguyen, Mihaly Varadi, Peter Tompa, and Kris Pauwels. PLOS one. 2017, 12 (3): e0174125. March 20, 2017. https://doi.org/10.1371/journal.pone.0174125.

Spontaneous Dissociation of Streptavidin from Biotinylated Double Stranded DNA. Kennedy Michelle. Undergraduate Honors Theses. 2017, pp 1-23. Spring 2017. http://scholar.colorado.edu/honr_theses/1373.

Allosteric Targeting of the Fanconi Anemia Ubiquitin-Conjugating Enzyme Ube2T by Fragment Screening. Francesca E Morreale, Alessio Bortoluzzi, Viduth K Chaugule, Connor Arkinson, Helen Walden, and Alessio Ciulli. Journal of Medicinal Chemistry. 2017, 60 pp 4093-4098. April 24, 2017. DOI: 10.1021/acs.jmedchem.7b00147.

Tip-Specific Functionalization of Gold Nanorods for Plasmonic Biosensing: Effect of Linker Chain Length. Pedro M R Paulo, Peter Zijlstra, Michel Orrit, Emilio Garcia-Fernandez, Tamara C S Pace, Ana S Viana, and Silvia M B Costa. Langmuir. 2017. June 7, 2017. DOI: 10.1021/acs.langmuir.7b00422.

Enzymatically active microspheres for self-propelled colloidal engines. Jungeum Park. CUNY Academic Works. 2017. http://academicworks.cuny.edu/cc_etds_theses/671.

IgY-binding peptide screened from a random peptide library as a ligand for IgY purification. Kamrul Hasan Khan, Arisa Himeno, Shouhei Kosugi, Yosuke Nakashima, Abdur Rafique, Ayana Imamura, Takaaki Hatanaka, Dai-Ichiro Kato, and Yuji Ito. Journal of Peptide Science. 2017, 23 (10) pp 790-797. July 31, 2017. DOI: 10.1002/psc.3027.

Improvement of Pharmacokinetic Profile of TRAIL via Trimer-Tag Enhances its Antitumor Activity in vivo. Haipeng Liu, Danmei Su, Jinlong Zhang, Shuaishuai Ge, Youwei Li, Fei Wang, Michel Gravel, Anne Roulston, Qin Song, Wei Xu, Joshua G. Liang, Gordon Shore, Xiaodong Wang, and Peng Liang. Scientific Reports. 2017, 7 (8953) pp 1-11. August 21, 2017. DOI: 10.1038/s41598-017-09518-1.

Comparison of homologous and heterologous prime-boost vaccine approaches using Modified Vaccinia Ankara and soluble protein to induce neutralizing antibodies by the human cytomegalovirus pentamer complex in mice. Flavia Chiuppesi, Felix Wussow, Louise Scharf, Heidi Contreras, Han Gao, Zhuo Meng, Jenny Nguyen, Peter A. Barry, Pamela J. Bjorkman, and Don J. Diamond. PLOS One. 2017, 12 (8) e0183377. August 16, 2017. https://doi.org/10.1371/journal.pone.0183377.

Force Measurement for Interaction between Cucurbit[7]uril and Mica and Self-Assembled Monolayer in the Presence of Zn2+ Studied with Atomic Force Microscopy. Young-In Bae, Iiha Hwang, Ikjin Kim, Kimoon Kim, and Joon Won Park. Langmuir. 2017, pp 1-32. September 26, 2017. DOI: 10.1021/acs.langmuir.7b02168.

Nonclassical Interactions of Phosphatidylcholine with Mucin Protect Intestinal Surfaces: A Microinterferometry Study. Federico Amadei, Benjamin Fröhlich, Wolfgang Stremmel, and Motomu Tanaka. Langmuir. 2018, 34 (46), pp 14046-14057. October 25, 2018. DOI: 10.1021/acs.langmuir.8b03035.

Competitive Immunoassays for the Detection of Small Molecules Using Single Molecule Arrays. Xu Wang, Limor Cohen, Jun Wang, and David R. Walt. Journal of the American Chemical Society. 2018. November 29, 2018. DOI: 10.1021/jacs.8b11185.

Quercetin Potentiates the NGF-Induced Effects in Cultured PC 12 Cells: Identification by HerboChips Showing a Binding with NGF. Gallant K. L. Chan, Winnie W. H. Hu, Zoey X. Zheng, M. Huang, Yan X. Y. Lin, Caroline Y. Wang, Amy G. W. Gong, X. Y. Yang, Karl W. K. Tsim, and Tina T. X. Dong. Evidence-Based Complementary and Alternative Medicine. 2018, 18 (1502457) pp 1-9. February 28, 2018. DOI: 10.1155/2018/1502457.

Structural basis for the specificity of renin-mediated angiotensinogen cleavage. Yahui Yan, Aiwu Zhou, Robin W. Carrell, and Randy J. Read. 2018. December 18, 2018. DOI: 10.1074/jbc.RA118.006608

Streptococcal dTDP‐L‐rhamnose biosynthesis enzymes: functional characterization and lead compound identification. Samantha L. van der Beek, Azul Zorzoli, Ebru Çanak, Robert N. Chapman, Kieron Lucas, Benjamin H. Meyer, Dimitrios Evangelopoulos, Luiz Pedro S. de Carvalho, Geert‐Jan Boons, Helge C. Dorfmueller, Nina M. van Sorge. Molecular Biology. 2019. Januaru 1, 2019. DOI: http://doi.org/10.1111/mmi.14197

Analytical validation of a novel multi-analyte plasma test for lung nodule characterization. Neil N Trivedi, James K Brown, Tess Rubenstein, Abigail D Rostykus, Amanda L Fish, Heng Yu, Luis Carbonell, Alice Juang, Sandy Kamer, Bhavin Patel, Manpreet Sidhu, Doris Vuong, Shan Wang, Mike Beggs, Alan HB Wu, and Mehrdad Arjomandi. Biomedical Research and Reviews. 2018, 2(3) pp 1-10. November 23, 2018. DOI: 10.15761/BRR.1000123

Time-dependent shotgun proteomics revealed distinct effects of an organoruthenium prodrug and its activation product on colon carcinoma cells. Samuel M. Meier-Menches, Katja Zappe, Andrea Bileck, Dominique Kreutz, Ammar Tahir, Margit Cichna-Markl, and Christopher Gerner. Metallomics. 2019, 11, 118-127. August 8, 2018. DOI: 10.1039/C8MT00152A.

Dynamics of the Eukaryotic Replicative Helicase at Lagging-Strand Protein Barriers Support the Steric Exclusion Model. Hazal B.Kose, Nicolai B.Larsen, Julien P.Duxin, HasanYardimci. Cell Reports. 2019, 26 (8) 2113-2125 February 19, 2019. https://doi.org/10.1016/j.celrep.2019.01.086

Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody. Van Son Nguyen, Laureen Logger , Silvia Spinelli, Aline Desmyter, Thi Thu Hang Le, Christine Kellenberger, Badreddine Douzi, Eric Durand, Alain Roussel, Eric Cascales, Christian Cambillau. PLoS ONE. 2015. March 26, 2015. https://doi.org/10.1371/journal.pone.0122187

Identification and characterization of Paracoccidioides lutzii proteins interacting with macrophages. Mariana Vieira Tomazett, Lílian Cristiane Baeza, Juliano Domiraci Paccez, Juliana Alves Parente-Rocha, Fátima Ribeiro-Dias, Célia Maria de Almeida Soares. Microbes and Infection. 2019. April 2, 2019. DOI: 10.1016/j.micinf.2019.03.002

Protein purification to analyze AAA+ proteolytic machine in vitro. Diego Rojas. Thesis, Columbia University: New York, NY, 2011. DOI: https://academiccommons.columbia.edu/doi/10.7916/D82B97H4.

A protein scaffold, engineered SPINK2, for generation of inhibitors with high affinity and specificity against target proteases. Daisuke Nishimiya, Yoshirou Kawaguchi, Shiho Kodama, Hatsumi Nasu, Hidenori Yano, Aya Yamaguchi, Masakazu Tamura & Ryuji Hashimoto. Scientific Report. 2019, Volume 9, Article number: 11436. August 7, 2019. DOI: 10.1038/s41598-019-47615-5

Glumac, Paige M.. (2019). Targeting CD133 In Androgen Receptor Indifferent, Neuroendocrine Differentiated Aggressive Variant Prostate Cancer. Retrieved from the University of Minnesota Digital Conservancy, http://hdl.handle.net/11299/206273.

Pyrrolobenzodiazepine Dimer Antibody–Drug Conjugates: Synthesis and Evaluation of Noncleavable Drug-Linkers. Stephen J. Gregson, Luke A. Masterson, Binqing Wei, Thomas H. Pillow, Susan D. Spencer, Gyoung-Dong Kang, Shang-Fan Yu, Helga Raab, Jeffrey Lau, Guangmin Li, Gail D. Lewis Phillips, Janet Gunzner-Toste, Brian S. Safina, Rachana Ohri, Martine Darwish, Katherine R. Kozak, Josefa dela Cruz-Chuh, Andrew Polson, John A. Flygare, and Philip W. Howard. Journal of Medical Chemistry. 2017, 60 (23) pp 9490-9507. 11/7/2017. DOI: 10.1021/acs.jmedchem.7b00736.

Potency determination of inactivated H7 influenza vaccines using monoclonal antibody-based ELISA and biolayer interferometry assays. Anupama Vasudevan, Amy Woerner, Falko Schmeisser, Swati Verma, Ollie Williams, and Jerry P. Weir. Influenza and Other Respiratory Viruses. 2017, 00 pp 1-9. 12/15/2017. DOI: 10.1111/irv.12528.

Exploring genotype to phenotype correlations in Duchenne muscular dystrophy. Camilla Johansson. EXAMENSARBETE INOM BIOTEKNIK, AVANCERAD NIVÅ, 30 HP. 2017, 00 pp i-vii. 5/26/2017.

Development and Validation of an Ultrasensitive Procalcitonin Sandwich Immunoassay. Viviana A. Carcamo Yañez, Jens C. Göpfert, Markus Otto, Hayrettin Tumani, Andreas Peter, and Thomas O. Joos. High-Throughput. 2017, 6 (4) pp 18. 11/16/2017. DOI: 10.3390/ht6040018.

Molecular characterization of human anti-hinge antibodies derived from single cell cloning of normal human B cells. Tao Huang, Mary Mathieu, Sophia Lee, Xinhua Wang, Yee Seir Kee, Jack J. Bevers III, Claudio Ciferri, Alberto Estavez, Manda Wong, Nancy Y. Chiang, Gerald Nakamura, and Randall J. Brezski. Journal of Biological Chemistry. 2017, 00 pp 1-20. November 30, 2017. DOI: 10.1074/jbc.RA117.000165.

Development and characterization of serotype-specific monoclonal antibodies against the dengue virus-4 (DENV-4) non-structural protein (NS1). Tesfaye Gelanew and Elizabeth Hunsperger. Virology Journal. 2018, 15 (30) pp 1-12. February 6, 2018. DOI: 10.1186/s12985-018-0925-7.

In vivo biotinylated calpastatin improves the affinity purification of human m-calpain. Hung Huy Nguyen, Alexander N. Volkov, Guy Vandenbussche, Peter Tompa, and Kris Pauwels. Protein Expression and Purification. 2018, 145, pp 77-84. January 13, 2018. DOI: 10.1016/j.pep.2018.01.002.

A Novel Antibody Targeting Tau Phosphorylated at Serine 235 Detects Neurofibrillary Tangles. David Brici, Jürgen Götz, and Rebecca M. Nisbet. Journal of Alzheimer's Disease. 2018, 61 (3) pp. 899-905. January 9, 2018. DOI: 10.3233/JAD-170610.

A natural human monoclonal antibody targeting Staphylococcus Protein A protects against Staphylococcus aureus bacteremia. Avanish K. Varshney, Galina A. Kuzmicheva, Jian Lin, Kevin M. Sunley, Rodney A. Bowling Jr., Tzu-Yu Kwan, Heather R. Mays, Anu Rambhadran, Yanfeng Zhang, Rebecca L. Martin, Michael C. Cavalier, John Simard, and Sushma Shivaswamy. PLOS ONE. 2018, 13 (1) pp 1-22. January 24, 2018. DOI: 10.1371/journal.pone.0190537.

The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition. Neetu M. Gulati, Andrzej S. Pitek, Anna E. Czapar, Phoebe L. Stewart, and Nicole F. Steinmetz. Journal of Materials Chemistry B. 2018, pp 1-14. February 27, 2018. DOI: 10.1039/C7TB03106H.

Characterization of Plasmodium Lactate Dehydrogenase and Histidine-Rich Protein 2 Clearance Patterns via Rapid On-Bead Detection from a Single Dried Blood Spot. Christine F. Markwalter, Lauren E. Gibson, Lwiindi Mudenda, Danielle W. Kimmel, Saidon Mbambara, Philip E. Thuma, and David W. Wright. The American Journal of Tropical Medicine and Hygiene. 2018, pp 1-28. March 18, 2018. DOI: 10.4269/ajtmh.17-0996.

Neutralizing Antibody Responses following Long-Term Vaccination with HIV-1 Env gp140 in Guinea Pigs. Christine A. Bricault, James M. Kovacs, Alexander Badamchi-Zadeh, Krisha McKee, Jennifer L. Shields, Bronwyn M. Gunn, George H. Neubauer, Fadi Ghantous, Julia Jennings, Lindsey Gillis, James Perry, Joseph P. Nkolola, Galit Alter, Bing Chen, Kathryn E. Stephenson, Nicole Doria-Rose, John R. Mascola, Michael S. Seaman, and Dan H. Barouch. Journal of Virology. 2018, pp 1-40. April 11, 2018. DOI: 10.1128/JVI.00369-18.

16kDa vasoinhibin binds to integrin alpha5 beta1 on endothelial cells to induce apoptosis. Kazunori Morohoshi, Ryo Mochinaga, Tsukasa Watanabe, Ryojun Nakajima, and Toshio Harigaya. Endocrine Connections. 2018, pp 1-30. April 5, 2018. DOI: 10.1530/EC-18-0116.

Lessons learned from merging wet lab experiments with molecular simulation to improve mAb humanization. L. Schwaigerlehner, M. Pechlaner, P. Mayrhofer, C. Oostenbrink, and R. Kunert. Protein Engineering, Design & Selection. 2018, pp 1-9. April 4, 2018. DOI: 10.1093/protein/gzy009.

Similar epitope specificities of IgG and IgA antibodies elicited by Ad26 vector prime, Env protein boost immunizations in rhesus monkeys. Zi Han Kang, Christine A. Bricault, Erica N. Borducchi, Kathryn E. Stephenson, Michael S. Seaman, Maria Pau, Hanneke Schuitemaker, Danielle van Manen, Frank Wegmann, and Dan H. Barouch. Journal of Virology. 2018, pp 1-34. May 23, 2018. DOI: 10.1128/JVI.00537-18.

Engineering Erythropoietin for Differential Receptor Binding Modes for Optimal Therapeutic Effects. Gruber, Nathalie. Masters Thesis, Harvard University, Boston, Massachusetts.

Measurement of Microcystin and Nodularin Activity in Human Urine by Immunocapture-Protein Phosphatase 2A Assay. Rebekah E. Wharton ,Brady R. Cunningham , Adam M. Schaefer, Sophia M. Guldberg, Elizabeth I. Hamelin and Rudolph C. Johnson. Toxins, 2019, 11(12), 729. December 13, 2019. DOI: 10.3390/toxins11120729

Generation of Lamprey Monoclonal Antibodies (Lampribodies) Using the Phage Display System. Khan M. A. Hassan, John D. Hansen, Brantley R. Herrin and Chris T. Amemiya. Biomolecules, 2019, 9, 868. December 12, 2019. DOI: 10.3390/biom9120868

The conformational distribution of a major facilitator superfamily peptide transporter is modulated by the membrane composition. Tanya Lasitza Male, Kim Bartels, Felix Wiggers, Gabriel Rosenblum, Jakub Jungwirth, Hagen Hofmann, Christian Löw. doi: https://doi.org/10.1101/2020.01.09.900316

A synthetic heparinoid blocks Tau aggregate cell uptake and amplification. Barbara E. Stopschinski, Talitha L. Thomas, Sourena Nadji, Eric Darvish, Linfeng Fan, Brandon B. Holmes, Anuja R. Modi, Jordan G. Finnell, Omar M. Kashmer, Sandi Estill-Terpack, Hilda Mirbaha, Hung S. Luu and Marc I. Diamond. The Journal of Biological Chemistry 295, 2974-2983. January 23, 2020. DOI: 10.1074/jbc.RA119.010353

A hydrodynamic instability drives protein droplet formation on microtubules to nucleate branches
Sagar U. Setru, Bernardo Gouveia, Raymundo Alfaro-Aco, Joshua W. Shaevitz, Howard A. Stone, Sabine Petry. February 3, 2020. arXiv:2001.06389




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NHS-dPEG®₄-biotin

$100.00 – $750.00

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NHS-dPEG®4-biotin, product number 10200, is a constant favorite among our customers. It has been used or featured in numerous publications over the years since it was first introduced. Biotin is normally poorly soluble in water, but the amphiphilic dPEG® linker imparts excellent solubility in water or aqueous buffer and in organic solvent. The N-hydroxysuccinimidyl (NHS) moiety of NHS-dPEG®4-biotin reacts with free primary amines, such as the amines on the side chain of lysine, forming a stable amide bond. The biotin moiety is used in labeling experiments, supramolecular construction, affinity chromatography, the creation of biotinylated antibodies, and many other applications.

Biotinylation with NHS-dPEG®4-biotin

In times past, aminocaproic acid was coupled to biotin and activated with NHS to form the widely used NHS-LC-biotin, but NHS-LC-biotin is highly problematic, as it is insoluble in water and must be dissolved in organic media prior to use. When coupled to proteins, the linker’s high degree of hydrophobicity can cause rapid aggregation of the proteins. A variant of NHS-LC-biotin, known as Sulfo-NHS-LC-biotin, is water soluble, but it still causes rapid aggregation of proteins.

NHS-dPEG®4-biotin does not cause protein aggregation. This permits experiments that require a high degree of biotin labeling without concerns about loss of protein due to aggregation. Dissolution of the product in organic solvent is not required for conjugation, thus providing easier reaction setup and greater reaction reproducibility. The dPEG® linker in PN10200 is slightly longer than the aminocaproic acid linker in LC-biotin; therefore, dPEG®4-biotin provides better access of the biotin to the avidin or streptavidin binding pocket compared to LC-biotin.

PN10200 is part of a family of dPEG®-biotin reagents that provide different lengths. The active esters can be either NHS or 2,3,5,6-tetrafluorophenol (TFP), which is more reactive towards primary amines and more stable in water or aqueous buffer. The optimum pH for reaction with NHS esters is in the range of 7.2 – 7.5, while for TFP esters, the optimum reaction pH range is slightly higher. Click here for an explanation of the advantages of TFP esters over NHS esters.

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If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

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Application References:

  1. Hermanson, G. T. Chapter 11, (Strept)avidin-Biotin Systems. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 465-505. Click here for a review of Greg’s book and a link to purchase it.
  2. Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 787-838.

Description

 

 

 

Additional information

Weight.5 oz
Dimensions.75 × .75 × 2 in

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