Biotin-dPEG®3-MAL, product number 10201, is one of three products containing biotin on one end of a dPEG® linker and a maleimidopropyl moiety (maleimide ring connected to propionic acid) on the other end. This product consists of a short-chain, single molecular weight, discrete PEG (dPEG®) spacer between a biotin group on one end and a thiol-reactive maleimide group on the other end.

Biotinylation and PEGylation

Biotinylation is the process of attaching biotin to a biomolecule or surface. This process finds extensive use in biotechnology, medical diagnostics, and many other applications. Typical biotinylation reagents such as LC-biotin are hydrophobic. This hydrophobicity can cause biotinylated biomolecules to precipitate from aqueous solutions. In turn, precipitation leads to a loss of activity for the bioconjugate molecule.

PEGylation is the process of modifying biomolecules and various surfaces with polyethylene glycol (PEG). PEG is soluble in both aqueous media and organic solvents (i.e., amphiphilic). Hydrophobic compounds gain water solubility and hydrodynamic volume when conjugated to PEG. Traditional PEG reagents are non-uniform, disperse polymers that contain multiple, different chain lengths of PEG, with each chain having a different molecular weight. The molecular weight of a traditional PEG is an average of all the different molecular weights of PEG found in the polymeric mixture.

Quanta BioDesign’s products are single molecular weight PEG products. For each product, there is only one molecular weight and a single chain length of PEG. Such PEG products are said to be “discrete.” We sell our discrete PEG products under the dPEG® tradename.

For more information on Quanta BioDesign’s dPEG® technology, please visit our “What is dPEG®?” page. For answers to our most frequently asked questions, please click here. To learn more about biotinylation with dPEG® products, please visit our “Biotinylation with dPEG® Products” page.

Using Biotin-dPEG®3-MAL

Maleimide groups can be conjugated to free thiol groups in the pH range from 6.5 – 7.5, forming a stable thioether linkage. Click here to learn more about maleimide chemistry. The dPEG® spacer in this product increases the hydrophilicity of the conjugate.

This product has proven useful in a variety of applications that take advantage of the high streptavidin-biotin binding affinity. Such applications include

  • FRET-based assays
  • probes of protein topology
  • protein labeling
  • the creation of biotinylated, dPEG®-modified nucleosides
  • different types of high-throughput screening procedures
  • the construction of biomimetic gold-and-silica nanoparticles, and
  • the construction of self-assembling quantum dots.

To use Biotin-dPEG®3-MAL, dissolve the product in a dry, water-miscible solvent and then add it to an aqueous solution of the compound containing the free thiol. N,N’-dimethylacetamide (DMAC) dried over 3Å molecular sieves is an excellent solvent for this purpose. For biomolecules (e.g., antibodies), use a minimal amount of the organic solvent. Otherwise, the biomolecule may denature, or the organic solvent may cause salt precipitation.

Commercial Scale Production Is Available for Biotin-dPEG®3-MAL

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Related Products

This product is one of three Biotin-dPEG®-MAL products with varying lengths of dPEG® spacers. Quanta BioDesign also offers a complete line of biotinylation products. The list of these products is here.

Act Now

Stop using inferior biotinylation reagents! Sure, many other people use LC-biotin products, but you can do better. Our biotinylation reagents offer

  • water solubility,
  • improved hydrodynamic volume,
  • no background noise (which means better signal), and
  • no protein precipitation caused by aggregation.

Why would you not use something better?

For better biotinylation, click the “Add to Cart” button now. You will not regret it.

Application References:

  1. Hermanson, G. T. Chapter 11, (Strept)avidin-Biotin Systems. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 465-505. Click here for a review of Greg’s book and a link to purchase it.
  2. Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 787-838.

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in