Specs:

 SDS

Molecular Weight: 597.73; single compound

CAS: 525573-22-2

Purity: > 98%

Spacers: dPEG® Spacer is 21 atoms and 24.9 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Solid Phase 4’-Phosphopantetheinylation: Fungal Thiolation Domains are Targets for Chemoenzymatic Modification, Deirdre Stack, Aisling Frizzell, Karen Tomkins, and Sean Doyle. Bioconjugate Chem. 2009, 20 (8), pp 1514–1522. July 23, 2009. DOI: 10.1021/bc900071j.

Manipulation of Carrier Proteins in Antibiotic Biosynthesis, James J. La Clair, Timothy L. Foley, Tracy R. Schegg, Conor M. Regan, and Michael D. Burkart. Chemistry & Biology. 2004. 11 (2), pp 195-201. February 2004. DOI: 10.1016/j.chembiol.2004.02.010.

Biomimetic approach to the formation of gold nanoparticle/silica cor/shell structures and subsequent bioconjugation, Sung Min Kang, Kyung-Bok Lee, Dong Jin Kim and Insung S Choi. Nanotechnology. 2006, (17), pp. 4719–4725. September 1, 2006. DOI: 10.1088/0957.

Probing the Topological Tolerance of Multimeric Protein Interactions: Evaluation of an Estrogen/Synthetic Ligand for FK506 Binding Protein Conjugate. Terry W. Moore Jillian R. Gunther, and John A. Katzenellenbogen. Bioconjugate Chem. 2010, 21 (10), pp 1880–1889. October 4, 2010. DOI: 10.1021/bc100266v.

Exploration of Dimensions of Estrogen Potency Parsing Ligand Binding and Coactivator Binding Affinities. M. Jeyakumar, Kathryn E. Carlson, Jillian R. Gunther, and John A. Katzenellenbogen. Journal of Biological Chemistry. 2011 (286), pp. 12971-12982. February 14, 2011. DOI: 10.1074/jbc.M110.205112.

Monitoring a Coordinated Exchange Process in a Four-Component Biological Interaction System: Development of a Time-Resolved Terbium-Based One Donor/Three-Acceptor Multi-Color FRET System. Sung Hoon Kim, Jillian R. Gunther, and John A. Katzenellenbogen. JACS. 2010 132 (13) pp 4685-4692. April 7, 2011. DOI:10.1021/ja100248q.

Diarylpropionitrile (DPN) Enantiomers: Synthesis and Evaluation of Estrogen Receptor β-Selective Ligands. Vincent M. Carroll, M. Jeyakumar, Kathryn E. Carlson, and John A. Katzenellenbogen. J. Med. Chem. 2012, 55 (1) pp 528–537. November 28, 2011. DOI: 10.1021/jm201436k.


Solid Phase 4′-Phosphopantetheinylation: Fungal Thiolation Domains are Targets for Chemoenzymatic Modification.Deirdre Stack, Aisling Frizzell, Karen Tomkins, and Sean Doyle. Bioconjugate Chem. 2009, 20 (8) pp 1514–1522. July 23, 2009. DOI: 10.1021/bc900071j.

Attachment of hydrogel microstructures and proteins to glass via thiol-terminated silanes. Jeong Hyun Seo, Dong-Sik Shin, Priam Mukundan, Alexander Revzin. Colloids and Surfaces B: Biointerfaces. 2012, 98, pp 1–6. October 1, 2012. doi.org/10.1016/j.colsurfb.2012.03.025.

A Set of Time-Resolved Fluorescence Resonance Energy Transfer Assays for the Discovery of Inhibitors of Estrogen Receptor-Coactivator Binding. Jillian R. Gunther, Yuhong Du, Eric Rhoden, Iestyn Lewis, Brian Revennaugh, Terry W. Moore, Sung Hoon Kim, Raymond Dingledine, Haian Fu and John A. Katzenellenbogen. Journal of Biomolecular Screening. 2009, 14 (2) pp # 181-195. February 4, 2009. DOI: 10.1177/1087057108329349.

Structural characterization and functionalization of engineered spider silk films. Kristina Spieß, Stefanie Wohlrab and Thomas Scheibel. Soft Matter. 2010, 6 (17) pp 4168-4174. June 16, 2010. DOI: 10.1039/b927267d.

Enzyme-independent, orientation-selective conjugation of whole human complement C3 to protein surfaces. Daniel A. Mitchell, Rebecca Ilyas, Alister W. Dodds, Robert B. Sim. Journal of Immunological Methods. 2008, 337 (1) pp 49-54. June 9, 2008. DOI: 10.1016/j.jim.2008.05.011.

Colorimetric multiplexed immunoassay using specific aggregation of antigenic peptide-modified luminous nanoparticles. Toshihiro Ihara, Yasunori Mori, Takaaki Imamura, Motoko Mukae, Shojiro Tanaka, Akinori Jyo. Science Direct. 2006, 578 (1) pp 11-18. May 4, 2006. DOI: 10.1016/j.aca.2006.04.066.

An Anti-Insulin-like Growth Factor I Receptor AntibodyIs a Potent Inhibitor of Cancer Cell Proliferation. Erin K. Maloney, Jennifer L. McLaughlin, Nancy E. Dagdigian. Cancer Research. 2003, 63 pp 5073-5083. August 15, 2003. DOI:

5’-Sulfhydryl-Modified RNA: Initiator Synthesis, in Vitro Transcription, and Enzymatic Incorporation. Lei Zhang, Lele Sun, Zhiyong Cui, Robert L. Gottlieb and Biliang Zhang. Bioconjugate Chem.2001, 12 (6) pp 939–948. October 16, 2001. DOI: 10.1021/bc015504g.

Sugar-Binding Proteins from Fish: Selection of High Affinity “Lambodies” That Recognize Biomedically Relevant Glycans. Xia Hong, Mark Z. Ma, Jeffrey C. Gildersleeve, Sudipa Chowdhury, Joseph J. Barchi, Jr., Roy A. Mariuzza,∥ Michael B. Murphy, Li Mao and Zeev Pancer. ACS Chem. Biol. 2013, 8 pp 152−160. October 2, 2012. doi.org/10.1021/cb300399s.

High-Throughput Screening of Small Molecules Identifies Hepcidin Antagonists. Eileen Fung, Priscilla Sugianto, Jason Hsu, Robert Damoiseaux, Tomas Ganz, and Elizabeta Nemeth. Mol Pharmacol. 2013, 83 (3) pp 681-690. January 4, 2013. DOI.org/10.1124/mol.112.083428.

Self-Assembled Quantum Dot-Bioconjugates: Characterization and Use for Sensing Proteolytic Activity. Igor L. Medintz1, Thomas Pons, Kim E. Sapsford, Philip E. Dawson and Hedi Mattoussi. Proc. of SPIE. 2008, 6945. April 15, 2008. DOI: 10.1117/12.782174.

Interleukin-2 signalling is modulated by a labile disulfide bond in the CD132 chain of its receptor. Clive Metcalfe, Peter Cresswell and A. Neil Barclay. Open Biology. 2012, 2 (1). January 11, 2012. DOI: 10.1098/rsob.110036.

Electrochemical Template Synthesis of Multisegment Nanowires:  Fabrication and Protein Functionalization. Bridget Wildt, Prashant Mali, and Peter C. Searson. Langmuir. 2006, 22 (25), pp 10528–10534. September 8, 2006. 10.1021/la061184j.

Targeted delivery of a photosensitizer to Aggregatibacter actinomycetemcomitans biofilm. Suci P, Kang S, Gmür R, Douglas T, Young M.. Antimicrobial Agents and Chemotherapy. 2010, 54 (6) pp 2489-2496. April 12, 2010. 10.1128/AAC.00059-10.




Additional Search Phrases:

Biotin-dPEG3-MAL, maleimide, maleimido, 52557322, PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, biotin, biotinylation, biotinylation reagent, biotin labeling, biotin label, maleimide, thiol, thiol reactive, sulfhydryl, Biotin-PEG3-MAL, Biotin-PEG3-maleimide, Maleimide-PEG3-biotin, Biotin-PEG-maleimide



 SDS    Datasheet

Concentration:

Format:

Buffer:

Preservative:

Storage:

Target Antigen:

Host:

Clonality:

Minimal Cross Reactivity To:

Country of Origin:

Label:

Ratio:

Excitation / Emission Maximum:

A280 Correction Factor:

Sensitivity:

Reactive Group:

Extinction Coefficient:

Spectrally Similar Dyes:

Molecular Weight:

Product Type:

pH:

Packaging:

Biotin-dPEG®₃-MAL

$140.00

Clear
PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses
Product#: 10201 Categories: , Tags: , , , , , , , , , , , , , , , , , ,

Biotin-dPEG®3-MAL, product number 10201, is one of three products containing biotin on one end of a dPEG® linker and a maleimidopropyl moiety (maleimide ring connected to propionic acid) on the other end. This product consists of a short-chain, single molecular weight, discrete PEG (dPEG®) spacer between a biotin group on one end and a thiol-reactive maleimide group on the other end.

Biotinylation and PEGylation

Biotinylation is the process of attaching biotin to a biomolecule or surface. This process finds extensive use in biotechnology, medical diagnostics, and many other applications. Typical biotinylation reagents such as LC-biotin are hydrophobic. This hydrophobicity can cause biotinylated biomolecules to precipitate from aqueous solutions. In turn, precipitation leads to a loss of activity for the bioconjugate molecule.

PEGylation is the process of modifying biomolecules and various surfaces with polyethylene glycol (PEG). PEG is soluble in both aqueous media and organic solvents (i.e., amphiphilic). Hydrophobic compounds gain water solubility and hydrodynamic volume when conjugated to PEG. Traditional PEG reagents are non-uniform, disperse polymers that contain multiple, different chain lengths of PEG, with each chain having a different molecular weight. The molecular weight of a traditional PEG is an average of all the different molecular weights of PEG found in the polymeric mixture.

Quanta BioDesign’s products are single molecular weight PEG products. For each product, there is only one molecular weight and a single chain length of PEG. Such PEG products are said to be “discrete.” We sell our discrete PEG products under the dPEG® tradename.

For more information on Quanta BioDesign’s dPEG® technology, please visit our “What is dPEG®?” page. For answers to our most frequently asked questions, please click here. To learn more about biotinylation with dPEG® products, please visit our “Biotinylation with dPEG® Products” page.

Using Biotin-dPEG®3-MAL

Maleimide groups can be conjugated to free thiol groups in the pH range from 6.5 – 7.5, forming a stable thioether linkage. Click here to learn more about maleimide chemistry. The dPEG® spacer in this product increases the hydrophilicity of the conjugate.

This product has proven useful in a variety of applications that take advantage of the high streptavidin-biotin binding affinity. Such applications include

  • FRET-based assays
  • probes of protein topology
  • protein labeling
  • the creation of biotinylated, dPEG®-modified nucleosides
  • different types of high-throughput screening procedures
  • the construction of biomimetic gold-and-silica nanoparticles, and
  • the construction of self-assembling quantum dots.

To use Biotin-dPEG®3-MAL, dissolve the product in a dry, water-miscible solvent and then add it to an aqueous solution of the compound containing the free thiol. N,N’-dimethylacetamide (DMAC) dried over 3Å molecular sieves is an excellent solvent for this purpose. For biomolecules (e.g., antibodies), use a minimal amount of the organic solvent. Otherwise, the biomolecule may denature, or the organic solvent may cause salt precipitation.

Commercial Scale Production Is Available for Biotin-dPEG®3-MAL

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Related Products

This product is one of three Biotin-dPEG®-MAL products with varying lengths of dPEG® spacers. Quanta BioDesign also offers a complete line of biotinylation products. The list of these products is here.

Act Now

Stop using inferior biotinylation reagents! Sure, many other people use LC-biotin products, but you can do better. Our biotinylation reagents offer

  • water solubility,
  • improved hydrodynamic volume,
  • no background noise (which means better signal), and
  • no protein precipitation caused by aggregation.

Why would you not use something better?

For better biotinylation, click the “Add to Cart” button now. You will not regret it.

Application References:

  1. Hermanson, G. T. Chapter 11, (Strept)avidin-Biotin Systems. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 465-505. Click here for a review of Greg’s book and a link to purchase it.
  2. Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 787-838.
Download Reagents Flyer

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

Related products