m-dPEG®₄-NHS ester




m-dPEG®4-NHS ester, product number 10211, is a short, methyl-terminated, discrete length polyethylene glycol (dPEG®) spacer. It is designed for modification of surfaces containing accessible free amines. The carboxyl terminus of the molecule is activated with N-hydroxysuccinimide (NHS). NHS reacts optimally with free amines at pH 7.2 – 7.5. Hydrolysis of the active ester to the carboxylic acid accelerates with increasing pH. Please note that modification of surface amines on biomolecules (e.g., proteins and peptides) with this uncharged, methyl-capped dPEG® spacer may alter the overall charge of the resulting conjugates.

Numerous applications employ this product. The applications in which this product has been successfully employed include:

  • cell surface engineering;
  • surface modification of biomolecules;
  • coating of carbon nanotubes, quantum dots, and other hydrophobic surfaces;
  • acoustic sensor development;
  • diagnostic films for pathogen detection, and,
  • various imaging applications.

m-dPEG®4-NHS ester, product number 10211, is just one member of an extensive line of methyl-terminated dPEG® products. This line includes dPEG® spacers containing 2 to 49 ethylene glycol units. These products are functionalized with NHS or with 2,3,5,6-tetrafluorophenol (TFP). TFP has proven to be superior to NHS for amine-reactive products. For more information on the superior performance of TFP esters, please see our page TFP Esters Have More Hydrolytic Stability and Greater Reactivity than NHS Esters.

Quanta BioDesign, Ltd., makes and sells single molecular weight PEG linkers and spacers with discrete chain lengths under the trade name dPEG®. Our products are distinctly different from traditional PEG products in that they are not disperse polymers. However, they confer all of the benefits of traditional PEG products. To learn more about dPEG® products, please read our page What is dPEG®? Please also visit our Frequently Asked Questions page to get answers to the questions we answer most often.





Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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