Fmoc-N-amido-dPEG®4-acid, product number 10213, is one of Quanta BioDesign’s perennially popular products for peptide synthesis. The dPEG®4 spacer allows the introduction of a short, hydrophilic spacer into a peptide chain.

PEGylation refers to the covalent addition of polyethylene glycol (PEG) to a compound or surface. PEGylated compounds include proteins, peptides, dyes or other labels, and small molecule drugs. Peptide synthesis frequently employs PEGylation to enhance the solubility of the peptide, because the amphiphilic character of PEG imparts tremendous water solubility to peptides. Moreover, peptide PEGylation prolongs the in vivo circulation of peptide conjugates by both increasing the peptide’s hydrodynamic volume and protecting the peptide from proteolysis. Additionally, PEGylation reduces or eliminates antigenicity of the conjugated peptide.

Traditional PEGs, however, are dispersed polymers (Đ > 1.00). A traditional PEG consists of multiple chain lengths with various molecular weights. The stated molecular weight for a conventional PEG indicates an average of the distribution of chain lengths and molecular weights.

Quanta BioDesign’s single molecular weight PEG products (known as “discrete PEG” and sold under the dPEG® trademark) provide all of the benefits of traditional, dispersed PEG without the analytical headache that comes from having an intractable mixture of conjugates, each with different PEG chain lengths. PN10213 permits our customers to insert a short (16 atoms) dPEG® into a peptide chain using familiar Fmoc chemistry. The product works equally well in solid phase and solution phase synthetic processes. The dPEG® can be inserted at either end of the peptide chain or in the middle of two amino acid sequences to provide a flexible spacer between distinct functional peptides. Additionally, the short dPEG® spacer can be used to provide extra distance in a synthetic construct where steric hindrance is a problem. The amphiphilic nature of dPEG® means that the construct will gain some degree of water solubility while remaining soluble in organic solvent. The Fmoc protecting group removes easily with a solution of piperidine in N,N-dimethylformamide (DMF).

To help our customers discover the optimum linker length necessary for a particular product or application, Fmoc-N-amido-dPEG®4-acid, product number 10213, is part of a line of Fmoc-protected amino-dPEG® acid products. The other products in this line include, PN10243, Fmoc-N-amido-dPEG®2-acid; PN10033, Fmoc-N-amido-dPEG®3-acid; PN10053, Fmoc-N-amido-dPEG®5-acid; PN10063, Fmoc-N-amido-dPEG®6-acid; PN10273, Fmoc-N-amido-dPEG®8-acid; PN10283, Fmoc-N-amido-dPEG®12-acid; PN10313, Fmoc-N-amido-dPEG®24-acid; PN10903, Fmoc-N-amido-dPEG®36-acid; and PN11301, Fmoc-amido-PEG®24-amido-dPEG®24-acid. In addition, we offer a line of Fmoc-protected amino acids that have already been activated with either N-hydroxysuccinimide (NHS) or 2,3,5,6-tetrafluorophenol (TFP). The NHS-activated products are PN10994, Fmoc-N-amido-dPEG®4-NHS ester; PN10995, Fmoc-N-amido-dPEG®8-NHS ester; and PN10996, Fmoc-N-amido-dPEG®12-NHS ester. The TFP-activated products are PN11000, Fmoc-N-amido-dPEG®4-TFP ester; PN11005, Fmoc-N-amido-dPEG®8-TFP ester; PN11006, Fmoc-N-amido-dPEG®12-TFP ester; PN11007, Fmoc-N-amido-dPEG®24-TFP ester; and PN11008, Fmoc-N-amido-dPEG®36-TFP ester. We invite you to consider our entire line of Fmoc-protected amino-dPEG®x-acid and active ester products whenever you need a single molecular weight PEG for your product. These products are available in prepackaged sizes of 100 mg and 1000 mg, but we can produce bulk amounts up to multiple kilogram amounts if you need larger quantities of these products.





Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in