MAL-dPEG®4-NHS ester, product number 10214, is consistently one of our best-selling products. MAL-dPEG®4-NHS ester is a crosslinking reagent that joins a sulfhydryl to a free amine. The sulfhydryl groups react with a maleimide group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the N-hydroxysuccinimidyl (NHS) ester of a carboxylic acid group. The maleimide and NHS functional groups on the crosslinking compound sit at either end of a short, discrete-length polyethylene glycol chain (dPEG®). The single molecular weight dPEG® spacer is 22 atoms and 24.8 Å long.
The reactions that join free amines to free thiols are among the most popular, most useful crosslinking reactions in bioconjugate chemistry. These reactions require heterobifunctional reagents that bridge the two different reactive groups. Traditional crosslinkers are hydrophobic molecules. Quanta BioDesign’s dPEG® crosslinking products are single molecular weight PEG compounds with discrete chain lengths.
The conjugation of conventional hydrophobic crosslinking reagents to biomolecules almost inevitably triggers problems such as aggregation and precipitation of the conjugates. These problems do not occur with our water-soluble, non-immunogenic dPEG® crosslinkers. For more information about our dPEG® products, please see our “What is dPEG®?” page. Also, click here for answers to our most frequently asked questions.
With these crosslinkers, the NHS ester end of the molecule must conjugate to a target molecule before the maleimide end of the molecule. At pH 7.0 – 7.5, NHS esters react optimally with free amines. However, NHS esters can react with free amines with pH as low as 6.0. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing MAL-dPEG®4-NHS ester, product number 10214, in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use.
The reaction of the maleimide end of MAL-dPEG®4-NHS ester, product number 10214, with a sulfhydryl proceeds optimally at pH 6.5 – 7.5. Use the lowest reasonable pH within this range. Above pH 7.5, free amines compete with free thiols at the maleimide reaction site, which can cause confusing results. Moreover, at higher pH values, the maleimide ring may open to form unreactive maleamic acid. For details about maleimide-thiol reaction chemistry, please click here.
The use of MAL-dPEG®4-NHS ester, product number 10214, has been published in numerous scientific paper and patents. The following list highlights some of the more important uses of this product:
Development of therapeutic antisense drugs;
Development of active and passively targeted nanoparticles;
Development of vaccines;
Development of nanoparticle-based photodynamic therapy;
Development of Gd-doped CuS nanoparticles for imaging and targeted photothermal treatment of gastric tumors;
What can you do with this product? Please click the “Add to Cart” button now to order MAL-dPEG®4-NHS ester, product number 10214.
 Hermanson, G. T. Chapter 6, Heterobifunctional Crosslinkers. In Bioconjugate Techniques, 3rd ed.; Academic Press: New York, NY, 2013; pp 299–340, specifically p. 300. Many scientists engaged in bioconjugation work consider Greg Hermanson’s book to be the definitive reference on the subject. Click here now to read a review of Greg’s book and to purchase it.
 Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. In Bioconjugate Techniques, 3rd ed.; Academic Press: New York, NY, 2013; pp 787–838, specifically p. 794.
 Hermanson, G. T. Chapter 6, op. cit., page 304.
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