Bis-MAL-dPEG®₃

$125.00$750.00

Clear

PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses

Bis-MAL-dPEG®3, product number 10215, is a short, homobifunctional, crosslinking reagent that links two molecules (e.g., peptides, proteins) together via the thiol-maleimide reaction (also known as the thiol-Michael addition). Maleimidopropionate groups functionalize each end of the molecule. A short, single molecular weight, discrete polyethylene glycol (dPEG®) spacer separates the two end groups.

The thiol-maleimide reaction, a type of click chemistry reaction, is a popular way to conjugate molecules. The maleimide functional group rapidly reacts with sulfhydryl groups, and the reaction is chemoselective for sulfhydryls in the pH range of 6.5 – 7.5. For details about the maleimide-thiol reaction, please see our Maleimide Reaction Chemistry page. The polyethylene glycol (PEG) spacer between the two maleimide groups is a single molecular weight compound with a discrete chain length (thus the tradename dPEG®). This product is not made from a dispersed polymer but is instead a single compound. For more information about Quanta BioDesign’s dPEG® technology, please click this link. Applications for this product include mapping which proteins in a complex are close together; determining ligand-receptor binding relationships; and construction of antibody-drug conjugates (ADCs), among many others.

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Application References:

  1. Hermanson, G. T. Chapter 3, The Reactions of Bioconjugation. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, pages 229 – 258, particularly page 241, where Greg writes about the maleimide reactive group. Want to learn more about Greg’s book? Click here now for a review of Greg’s book and a link to purchase it.
  2. Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, pages 787 – 838.
  3. Choe, S. H.; Kim, J. E.; Lee, Y. C.; Jang, Y. J.; Choe, M. H. A Divalent Immunotoxin Formed by the Disulfide Bond between Hinge Regions of Fab Domain. Bulletin of the Korean Chemical Society 2001, 22(12), 1361–1365. http://www.koreascience.or.kr/article/JAKO200113464477984.page
  4. Giron-Monzon, L.; Manelyte, L.; Ahrends, R.; Kirsch, D.; Spengler, B.; Friedhoff, P. Mapping Protein-Protein Interactions between MutL and MutH by Cross-Linking. J. Biol. Chem. 2004, 279(47), 49338–49345. https://doi.org/10.1074/jbc.M409307200.
  5. Pan, H.; Yang, J.; Kopečková, P.; Kopeček, J. Backbone Degradable Multiblock N-(2-Hydroxypropyl)Methacrylamide Copolymer Conjugates via Reversible Addition−Fragmentation Chain Transfer Polymerization and Thiol−ene Coupling Reaction. Biomacromolecules 2011, 12(1), 247–252. https://doi.org/10.1021/bm101254e.
  6. Ravasco, J. M. J. M.; Faustino, H.; Trindade, A.; Gois, P. M. P. Bioconjugation with Maleimides: A Useful Tool for Chemical Biology. Chemistry – A European Journal 2019, 25(1), 43–59. https://doi.org/10.1002/chem.201803174.
  7. Northrop, B. H.; Frayne, S. H.; Choudhary, U. Thiol–Maleimide “Click” Chemistry: Evaluating the Influence of Solvent, Initiator, and Thiol on the Reaction Mechanism, Kinetics, and Selectivity. Polym. Chem. 2015, 6(18), 3415–3430. https://doi.org/10.1039/C5PY00168D.

Additional information

Weight.5 oz
Dimensions.75 × .75 × 2 in