Specs:

 SDS

Molecular Weight: 708.71; single compound

CAS: 1008402-79-6

Purity: > 97%

Spacers: dPEG® Spacer is 31 atoms and 35.7 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Hermanson, G. T. Chapter 5, Homobifunctional Crosslinkers. In Bioconjugate Techniques, 3rd edition; Academic Press: New York, NY, 2013; pp 275–298.

Potent D-peptide inhibitors of HIV-1 entry. Brett D. Welch, Andrew P. VanDemark, Annie Heroux, Christopher P. Hill, and Michael S. Kay. PNAS. 2007, 104 (43) pp 16828-16833. October 23, 2007. DOI: 10.1073/pnas.0708109104.

Dynamic electromagnetophoretic force analysis of a single binding interaction between lectin and mannan polysaccharide on yeast cell surface. Yoshinori Iigunia and Hitoshi Watarai. Analyst. 2010, 135 (6) pp 1426–1432. March 16, 2010. DOI: 10.1039/b924339a.

Orientation-regulated immobilization of Jagged1 on glass substrates for ex vivo proliferation of a bone marrow cell population containing hematopoietic stem cells. Hiroyuki Toda, Masaya Yamamoto, Hiroshi Kohara, Yasuhiko Tabata. Biomaterials. 2011, 32 (29) pp 6920-6928. October 2011. DOI: 10.1016/j.biomaterials.2011.05.093.

Cyclic and dimeric gluten peptide analogues inhibiting DQ2-mediated antigen presentation in celiac disease. Jiang Xia, Elin Bergseng, Burkhard Fleckenstein, Matthew Siegel, Chu-Young Kim, Chaitan Khosla and Ludvig M. Sollid. Bioorganic & Medicinal Chemistry. 2007, 15 (20) pp 6565–6573. October 15, 2007. DOI: 10.1016/j.bmc.2007.07.001.

Phosphoramidate and phosphate prodrugs of (_)-b-D-(2R,4R)-dioxolane-thymine: Synthesis, anti-HIV activity and stability studies. Yuzeng Liang, Janarthanan Narayanasamy, Raymond F. Schinazib and Chung K. Chua. Bioorganic & Medicinal Chemistry. 2006, 14 (7) pp 2178–2189. April 1, 2006. DOI: 10.1016/j.bmc.2005.11.008.

Mapping of Molecular Structure of the Nanoscale Surface in Bio-nanoparticles. Luciana M Herda, Delyan R Hristov, Maria Cristina Lo Giudice, Ester Polo, and Kenneth A Dawson. Journal of the American Chemical Society. 2017. 139(1), 111-114. DOI: 10.1021/jacs.6b12297.

Additional Search Phrases:

Bis-dPEG9-NHS ester, n-hydroxysuccinimide, 1008402796, PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, crosslinker, cross linker, crosslinking reagent, amine reactive, homobifunctional, NHS, N-hydroxysuccinimide, NHS ester, N-hydroxysuccinimide ester, active ester, Bis-PEG9-NHS ester, NHS-PEG9-NHS ester, Bis(succinimidyl) nona(ethylene glycol), BS(PEG)9, Bis-NHS ester-PEG



 SDS    Datasheet

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Bis-dPEG®₉-NHS ester

$225.00 – $550.00

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PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses
Product#: 10246 Categories: ,

TFP_Esters_Art2

Bis-dPEG®9-NHS ester, product number 10246, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Bis-dPEG®9-NHS ester, product number 10246, has been published in the scientific literature. A list of relevant publications for this product appears at the end of this product description.

Homobifunctional Reagents

Homobifunctional reagents are chemical compounds that contain two identical reactive groups. Symmetrical homobifunctional reagents were among the first crosslinkers developed for conjugation of biomolecules. These were typically hydrophobic compounds, and their use – particularly in one-step reactions – often was accompanied with problems such as inactivation and precipitation of the conjugated biomolecules. Greg Hermanson’s comprehensive reference work Bioconjugate Techniques has an entire chapter (Chapter 5) devoted to homobifunctional reagents. Quanta BioDesign, Ltd. recommends and sells this authoritative reference.

Quanta BioDesign’s products are amphiphilic. Therefore, they impart water solubility to molecules to which they are conjugated. Consequently, when used in crosslinking reactions, Bis-dPEG®9-NHS ester will not cause problems such as aggregation, precipitation, and inactivation. For a thorough explanatory discussion of dPEG® products, please visit our “What is dPEG®?” page. For answers to our frequently asked questions, please click this link to visit our page “Frequently Asked Questions (about dPEG® products).”

Using Bis-dPEG®9-NHS ester

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.0. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases.

Thus, we strongly discourage storing Bis-dPEG®9-NHS ester, product number 10246, in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Commercial Scale Production of Bis-dPEG®9-NHS ester Is Available

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Buy It Now!

Hydrophobic crosslinkers trigger aggregation and precipitation of biomolecules and contribute to problems with non-specific binding. Polymeric PEG crosslinkers eliminate the issues caused by hydrophobic crosslinkers, but they create new, difficult challenges in analyzing and characterizing the crosslinked conjugates.

You can solve all of those problems right now by clicking on the “Add to Cart” button and purchasing Bis-dPEG®9-NHS ester. The monodispersed PEG crosslinker provides water solubility and larger hydrodynamic volume to the conjugate molecules. However, because it is a discrete compound, not a polymer, the analytical challenges caused by dispersed polymers disappear.

So stop using inferior crosslinkers! Click the “Add to Cart” button now to order Bis-dPEG®9-NHS ester.

Description

 

 

 

 

 

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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