Specs:

 SDS

Molecular Weight: 685.75; single compound

CAS: 756525-94-7

Purity: > 98%

Spacers: dPEG® Spacer is 38 atoms and 44.7 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The impact of molecular Weight and PEG Chain Length on the Systemic Pharmacokinetics of PEGylated Poly L-Lysine Dendrimers. Lisa M. Kaminskas, Ben J. Boyd, Peter Karellas, Guy Y. Krippner, Romina Lessene, Brian Kelly, and Christopher J. H. Porter. Molecular Pharmaceutics. 2008, 5 (3), pp 449–463. April 5, 2008. DOI: 10.1021/mp7001208.

Pharmacokinetics and Tumor Disposition of PEGylated Methotrexate Conjugated Poly-L-lysine Dendrimers. Lisa M. Kaminskas, Brian D. Kelly, Victoria M. McLeod, Ben J. Boyd, Guy Y. Krippner, Elizabeth D. Williams, and Christopher J. H. Porter, Molecular Pharmaceutics, Vol. 6, No. 4, 2009, 6 (4), pp 1190-1204, May 19, 2009. DOI: 10.1021/mp900049a.

Hairpin Structure of a Biarsenical- Tetracysteine Motif Determined by NMR Spectroscopy. Fatemeh Madani, Jesper Lind, Peter Damberg, Stephen R. Adams, Roger Y. Tsien, and Astrid O. Graslund. Journal of the American Chemical Society. 2009, 131 (13), pp 4613–4615. March 12, 2009. DOI: 10.1021/ja809315x.

Improved pharmacokinetics and immunogenicity profile of organophosphorus hydrolase by chemical modification with polyethylene glycol. Boris N. Novikov, Janet K. Grimsley, Rory J. Kern, James R. Wild, Melinda E. Wales. Journal of Controlled Release. 2010, 146 (3) pp 318-325. September 15, 2010. DOI: 10.1016/j.jconrel.2010.06.003.

Antibacterial activity and cytotoxicity of PEGylated poly(amidoamine) dendrimers. Analette I. Lopez, Rose Y. Reins, Alison M. McDermott, Barbara W. Trautner and Chengzhi Cai. Molecular BioSystems. 2009, 10 (5) pp 1148–1156. May 26, 2009. DOI: 10.1039/b904746h.

Stability, Antimicrobial Activity, and Cytotoxicity of Poly(amidoamine) Dendrimers on Titanium Substrates. Lin Wang, Uriel J. Erasquin, Meirong Zhao, Li Ren, Martin Yi Zhang, Gary J. Cheng, Yingjun Wang, and Chengzhi Cai. ACS Applied Materials & Interfaces. 2011, 3 (8), pp 2885-2894. July 20, 2011. DOI: 10.1021/am2004398.

Prevention of benzyl alcohol-induced aggregation of chymotrypsinogen by PEGylation. José A. Rodríguez-Martínez, Izarys Rivera-Rivera and Kai Griebenow. Journal of Pharmacy and Pharmacology. 2011, 63 (6), pp 800–805. January 6, 2011. DOI: 10.1111/j.2042-7158.2011.01288.

Development and Application of a Multimodal Contrast Agent for SPECT/CT Hybrid Imaging. Jason M. Criscione, Lawrence W. Dobrucki, Zhen W. Zhuang, Xenophon Papademetris, Michael Simons, Albert J. Sinusas and Tarek M. Fahmy. Bioconjugate Chemistry. 2011, 22 (9), pp 1784–1792. August 18, 2011. DOI: 10.1021/bc200162r.

Antibacterial Activities of Poly (amidoamine) Dendrimers Terminated with Amino and Poly(ethylene glycol) Groups. Michelle K. Calabretta, Amit Kumar, Alison M. McDermott, and Chengzhi Cai. Biomacromolecules. 2007, 8 (6) pp 1807-1811. May 19, 2007. DOI: 10.1021/bm0701088.

Enhancement of the CD8+ T cell response to a subdominant epitope of respiratory syncytial virus by deletion of an immunodominant epitope. Hoyin Mok, Sujin Lee, David W. Wright, and James E. Crowe Jr. Vaccine. 2008, 26 (37) pp 4775-4782. September 2, 2008. DOI: 10.1016/j.vaccine.2008.07.012.

Silica-Shelled Single Quantum Dot Micelles as Imaging Probes with Dual or Multimodality. Rumiana Bakalova, Zhivko Zhelev, Ichio Aoki, Hideki Ohba, Yusuke Imai, and Iwao Kanno. Analytical Chemistry. 2006, 78 (16) pp 5925–5932. July 14, 2006. DOI: 10.1021/ac060412b.

Multifunctional Separation Mechanism on Poly(oxyethylene) Stationary Phases in Capillary Liquid Chromatography. Toyohide Takeuchi and Lee Wah Lim. Analytical Sciences. 2010, 26 (9) pp 937-941. September 10, 2010. DOI: 10.2116/analsci.26.937.

N-Alkylated aminopyrazines for use as hydrophilic optical agents. Amruta R. Poreddy, Bethel Asmelash, Karen P. Galen, Richard M. Fitch, Jeng-Jong Shieh, James M. Wilcox, Tasha M. Schoenstein, Jolette K. Wojdyla, Kimberly R. Gaston, John N. Freskos, William L. Neumann, Raghavan Rajagopalan, Hyo-Yang Ahn, James G. Kostelc, Martin P. Debreczeny, Kevin D. Belfield, and Richard B. Dorshow. SPIE. Digital Library, Optical Molecular Probes and Industry. 2009. 7190. January 24, 2009. DOI: 10.1117/12.809287.

Reconstitution of the M13 Major Coat Protein and Its Transmembrane Peptide Segment on a DNA Template. Weijun Li, Itai Suez and Francis C. Szoka, Jr. ACS Biochemistry. 2007, 46 (29) pp 8579–8591. June 27, 2007. DOI: 10.1021/bi700165m.

Acid-degradable cationic methacrylamide polymerized in the presence of plasmid DNA as tunable non-viral gene carrier. In Kap Ko, Assem Ziady, Shiwei Lu, Young Jik Kwon. Biomaterials. 2008, 29 (28) pp 3872–3881. June 27, 2008. DOI: 10.1016/j.biomaterials.2008.06.003.

Electrostatic readout of DNA microarrays with charged microspheres. Nathan G Clack, Khalid Salaita, and Jay T Groves. Nature Biotechnology. 2008, 26 (7) pp 825-830. June 29, 2008. DOI: 10.1038/nbt1416.

Poly(ethylene oxide)-bonded stationary phase for capillary ion chromatography. Toyohide Takeuchi, Budhi Oktavia, Lee Wah Lim. Anal Bioanal Chem. 2009, 393(4) pp 1267-1272. November 30, 2008. DOI: 10.1007/s00216-008-2533-7.

An adsorption chromatography assay to probe bulk particle transport through hydrogels. I. Vladescu, O. Lieleg, S. Jang, and Katharina Ribbeck. Journal of Pharmaceutical Sciences. 2012, 101 (1) pp 436-442. September 8, 2011. DOI: 10.1002/jps.2273.

Biomolecular strategies for cell surface engineering. John Tanner Wilson. Smartech. 2008, December 5, 2008.

Investigations into Improving the Separation of PEGylated Proteins. Vita Knudson, Tividar Farkas, and Michael McGinley. Phenomenex, (TN-1034).

Stabilization of α-chymotrypsin upon PEGylation correlates with reduced structural dynamics. Jose A. Rodriguez-Martinez, Ricardo J. Sola, Betzaida Castillo, Hector R. Cintron-Colon, Izarys Rivera-Rivera, Gabriel Barletta, Kai Griebenow. Biotechnology and Bioengineering. 2008, 101 (6) pp 1142-1149. December 15, 2008. DOI: 10.1002/bit.22014.

Development of a widefield SERS imaging endoscope. Patrick Z. McVeigh, Rupananda J. Mallia, Israel Veilleux, Brian C. Wilson. SPIE Proceedings. 2012, 8217 (1) pp 1-6. February 13, 2012. DOI: 10.1117/12.907304.

Characterization of Particle Translocation through Mucin Hydrogels. Oliver Lieleg, Ioana Vladescu, and Katharina Ribbeck. Biophysical Journal. 2010, 98 (9) pp 1782–1789. May 5, 2010. DOI: 10.1016/j.bpj.2010.01.012.

Short polyethylene glycol chains densely bound to soft nanotube channels for inhibition of protein aggregation. N. Kameta, T. Matsuzawa, K. Yaoi, and M. Masuda. RSC Advances. 2016, 6 (43) pp 36744-36750. April 7, 2016. DOI: 10.1039/C6RA06793J.

Site directed Pegylation: Role of pH and temperature, to increase the productivity of insulin oligomer conjugate. Ashutosh Sudhir Naik, Koduru srivatsa, Krishnappa Mane, Phanichand Kodali, Laxmi Adhikary. Journal of Pharmaceutical and Biological Sciences. 2017, 5(4) pp 155-160. 2017. https://innovativepublication.com/admin/uploaded_files/JPBS_5(4)_155-160.pdf

Protease-triggered siRNA delivery vehicles. David B. Rozema, Andrei V. Blokhin, Darren H. Wakefield, Jonathan D. Benson, Jeffrey C. Carlson, Jason J. Klein, Lauren J. Almeida, Anthony L. Nicholas, Holly L. Hamilton, Qili Chu, Julia O. Hegge, So C. Wong, Vladimir S. Trubetskoy, Collin M. Hagen, Eric Kitas, Jon A. Wolff, and David L. Lewis. Journal of Controlled Release. 2017, 209 pp 57-66. 7/10/2015. DOI: 10.1016/j.jconrel.2015.04.012.

Multivalent Benzamidine Molecules for Plasmin Inhibition: Effect of Valency and Linker Length. Tanmaye Nallan Chakravarthula, Dr. Ziqian Zeng, Prof. Nathan J. Alves. ChemMedChem, Volume 17, Issue 22, (2022), 09/16/2022, https://doi.org/10.1002/cmdc.202200364.

Additional Search Phrases:

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 SDS    Datasheet

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m-dPEG®₁₂-NHS ester

$175.00 – $600.00

Clear
PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses
Product#: 10262 Categories: , Tags: , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,

TFP_Esters_Art2

m-dPEG®12-NHS ester, product number 10262, is a medium length, methyl-terminated, discrete length polyethylene glycol (dPEG®) spacer. With a reactive end as the N-hydroxysuccinimidyl (NHS) ester, m-dPEG®12-NHS ester is designed to modify surfaces containing accessible free amines. Except for the reactive ester, this product is essentially identical to m-dPEG®12-TFP ester, product number 10306.

NHS reacts optimally with free amines at pH 7.2 – 7.5. In aqueous media, the hydrolytic rate of the ester to the carboxylic acid increases with increasing pH. Reacting surface amines on biomolecules (e.g., proteins and peptides) with this uncharged, methyl-capped dPEG® spacer may alter the overall charge of the resulting conjugates.

Many applications employ this m-dPEG®12-NHS ester. Published papers using this product include uses in:

  • vaccine development;
  • cell surface engineering;
  • multimodal imaging;
  • stable antimicrobial, cytotoxic dendrimers;
  • nanoparticles for siRNA delivery;
  • coating of nanoparticles, quantum dots, and carbon nanotubes;
  • PK and immunogenicity improvements for organophosphorus hydrolase;
  • PK and immunogenicity improvements for poly-L-lysine dendrimers used as drug delivery vehicles to tumors; and,
  • preventing enzyme aggregation.

m-dPEG®12-NHS ester, product number 10262, is just one member of an comprehensive line of methyl-terminated dPEG® products. This line includes dPEG® spacers containing 2 to 49 ethylene glycol units. These products are functionalized with NHS or with 2,3,5,6-tetrafluorophenol (TFP). TFP has proven to be superior to NHS for amine-reactive products. For more information on the superior performance of TFP esters, please see our page TFP Esters Have More Hydrolytic Stability and Greater Reactivity than NHS Esters.

Quanta BioDesign, Ltd., makes and sells single molecular weight PEG linkers and spacers with discrete chain lengths under the trade name dPEG®. Our products are distinctly different from traditional PEG products in that they are not disperse polymers. However, they confer all of the benefits of traditional PEG products. To learn more about dPEG® products, please read our page What is dPEG®? Please also visit our Frequently Asked Questions page to get answers to the questions we answer most often.

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Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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