m-dPEG®₁₂-amine

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m-dPEG®12-amine, product number 10288, is a non-immunogenic, water-soluble chemical modification reagent. The product contains a single molecular weight, discrete-length polyethylene glycol (dPEG®) chain that is 38 atoms (43.9 Å) long. The dPEG® chain terminates on one end with a methyl group and the other end with an amine. The amino group reacts with carboxylic acids (and their active esters), aldehydes, and ketones. Reacting the amino group with aldehydes or ketones forms a labile imine (Schiff base). Reacting carboxylic acids or their active esters with m-dPEG®12-amine forms stable amide bonds. Uses for this product include passivating surfaces and coating biomolecules.

PEGylation with dPEG® Products

PEGylation is the process of modifying surfaces, small molecules, and biomolecules such as peptides or proteins, with polyethylene glycol (PEG). Conventional PEG reagents are disperse polymers. Polymer dispersity complicates the analysis of conjugates created with them. Quanta BioDesign’s dPEG® products have no dispersity. Instead, each dPEG® product contains a discrete chain length of PEG with a single molecular weight. For a thorough discussion of our dPEG® technology, please visit our “What Is dPEG®?” page. For answers to our most frequently asked questions, please visit this page.

Uses of m-dPEG®12-amine

m-dPEG®12-amine proves useful in surface passivation and coating biomolecules. Biomolecules modified with m-dPEG®12-amine display enhanced water solubility, increased hydrodynamic volume, and reduced immunogenicity. This product reacts quickly with active esters of carboxylic acids (for example, NHS esters and TFP esters) to form amide bonds. Also, it forms amide bonds directly with carboxylic acids using the carbodiimide EDC. Greg Hermanson has published protocols for coupling amines with carboxylic acids, NHS and TFP esters, aldehydes, and ketones in Bioconjugate Techniques, 3rd edition.

Several publications in the scientific literature report the successful use of m-dPEG®12-amine. Published reports using this product include:

  • cancer imaging with a NIR-fluorescent peptosome;
  • multimodal imaging and targeted gene silencing;
  • noncompetitive on-chip immunoassays;
  • antibody pretargeting; and,
  • the development of biocompatible quantum dots.

What will you do with this product?

Commercial Scale Product of m-dPEG®12-amine is Available

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Buy Now!

Traditional disperse polymer PEG products add unnecessary analytical complexity to conjugates that incorporate them. Quanta BioDesign’s dPEG® products remove that analytical problem because all dPEG® products have a discrete chain length and a single molecular weight. Our products provide all of the benefits of PEG with none of the headaches that polymer PEGs bring.

Stop using inferior products!

Start using single molecular weight dPEG® products and discover the dPEG® difference. To get started, click the “Add to Cart” button now and drop m-dPEG®12-amine in your shopping basket today.

Application Reference:

Greg Hermanson. Bioconjugate Techniques, 3rd Edition. New York: Academic Press, 2013, 1146 pp.

Multiple chapters throughout the book contain reaction protocols. Consult the index for specific page numbers. The most useful procedures are in Chapter 4, “Zero-Length Crosslinkers;” Chapter 5, “Homobifunctional Crosslinkers;” and Chapter 6, “Heterobifunctional Crosslinkers.” Be sure also to check out Chapter 18, “PEGylation and Synthetic Polymer Modification,” where Greg discusses Quanta BioDesign’s dPEG® products in detail.

Many scientists regard Greg’s book as the most authoritative text for bioconjugation work. Quanta BioDesign offers Bioconjugate Techniques, 3rd edition, at a special price. To read a review of the book and to purchase it, please click this link.

Description

 

 

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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