m-dPEG®₂₄-TFP ester




m-dPEG®24-TFP ester, product number 10303, is a long, methyl-terminated, discrete length polyethylene glycol (dPEG®) spacer. With a reactive end of 2,3,5,6-tetrafluorophenyl (TFP) ester, m-dPEG®24-TFP ester is designed to modify surfaces containing accessible free amines. Except for the reactive end group, this product is identical to m-dPEG®24-NHS ester, product number 10304. This product is closely related to m-dPEG®25-TFP ester, product number 11290.

Free amines react optimally with TFP esters at pH 7.5 – 8.0 in aqueous media. TFP esters are more hydrolytically stable in water and aqueous buffer than NHS esters. Nevertheless, in aqueous media, the rate at which the ester hydrolyzes to the carboxylic acid increases as the pH increases. Moreover, it should be noted that reacting surface amines on biomolecules (e.g., proteins and peptides) with this uncharged, methyl-capped dPEG® spacer may alter the overall charge of the resulting conjugates.

There are many applications that could potentially, successfully use m-dPEG®24-TFP ester. Among those applications are:

  • cell surface engineering;
  • PK, BD, and immunogenicity profile improvements for biomolecules;
  • imaging applications by reducing background and non-specific binding;
  • dendrimer construction;
  • nanoparticle, quantum dot, and carbon nanotube surface coating; and,
  • prevention of protein aggregation.

m-dPEG®24-TFP ester, product number 10303, is just one member of a broad line of methyl-terminated dPEG® products. This line includes dPEG® spacers containing 2 to 49 ethylene glycol units. These products are functionalized with NHS or TFP. In addition to better stability to hydrolysis, TFP esters have proven to be superior to NHS esters for amine-reactive products. For more information on the superior performance of TFP esters, please see our page TFP Esters Have More Hydrolytic Stability and Greater Reactivity than NHS Esters.

Quanta BioDesign, Ltd., makes and sells single molecular weight PEG linkers and spacers with discrete chain lengths under the trade name dPEG®. Our products are distinctly different from traditional PEG products in that they are not disperse polymers. However, they confer all of the benefits of traditional PEG products. To learn more about dPEG® products, please read our page What is dPEG®? Please also visit our Frequently Asked Questions page to get answers to the questions we answer most often.

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