m-dPEG®₁₂-TFP ester




m-dPEG®12-TFP ester, product number 10306, is a medium length, methyl-terminated, discrete length polyethylene glycol (dPEG®) spacer. With a reactive end as the 2,3,5,6-tetrafluorophenyl (TFP) ester, m-dPEG®12-TFP ester is designed to modify surfaces containing accessible free amines. Except for the reactive ester, this product is essentially identical to m-dPEG®12-NHS ester, product number 10262.

TFP esters react optimally with free amines at pH 7.5 – 8.0. Compared to N-hydroxysuccinimidyl (NHS) esters, TFP esters have greater hydrolytic stability in aqueous media. However, as with NHS esters, the hydrolytic rate of the ester to the carboxylic acid increases as the pH rises. Reacting surface amines on biomolecules (e.g., proteins and peptides) with this uncharged, methyl-capped dPEG® spacer may alter the overall charge of the resulting conjugates.

There are numerous applications that could successfully employ m-dPEG®12-TFP ester. Applications that could use this product include the following:

  • vaccine development;
  • cell surface engineering;
  • imaging applications to reduce background and non-specific interactions;
  • construction of dendrimers;
  • coating of nanoparticles, quantum dots, and carbon nanotubes;
  • improving PK and BD of biomolecules; and,
  • preventing enzyme aggregation.

m-dPEG®12-TFP ester, product number 10306, is just one member of an comprehensive line of methyl-terminated dPEG® products. This line includes dPEG® spacers containing 2 to 49 ethylene glycol units. These products are functionalized with TFP or NHS. TFP has proven to be superior to NHS for amine-reactive products. For more information on the superior performance of TFP esters, please see our page TFP Esters Have More Hydrolytic Stability and Greater Reactivity than NHS Esters.

Quanta BioDesign, Ltd., makes and sells single molecular weight PEG linkers and spacers with discrete chain lengths under the trade name dPEG®. Our products are distinctly different from traditional PEG products in that they are not disperse polymers. However, they confer all of the benefits of traditional PEG products. To learn more about dPEG® products, please read our page What is dPEG®? Please also visit our Frequently Asked Questions page to get answers to the questions we answer most often.

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