MAL-dPEG®₂₄-NHS ester

$250.00$1,000.00

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PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses

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MAL-dPEG®24-NHS ester, product number 10314, is a crosslinking reagent that joins a sulfhydryl to a free amine. The sulfhydryl groups react with a maleimide group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the N-hydroxysuccinimidyl (NHS) ester of a carboxylic acid group. The maleimide and NHS functional groups on the crosslinking compound sit at either end of a long, discrete-length polyethylene glycol chain (dPEG®).

The joining of free amines to sulfhydryl groups is one of the most popular[1], most useful[2] crosslinking reactions in bioconjugate chemistry. These reactions require heterobifunctional reagents that bridge the two groups. Traditional crosslinkers are hydrophobic molecules. Quanta BioDesign’s dPEG® crosslinking products are water-soluble, amphiphilic, single molecular weight PEG compounds with discrete chain lengths.

The conjugation of conventional hydrophobic crosslinking reagents to biomolecules almost inevitably triggers problems such as aggregation and precipitation of the conjugates. These problems do not occur with our water-soluble, non-immunogenic dPEG® crosslinkers. For more information about our dPEG® products, please see our “What is dPEG®?” page. Also, click here for answers to our most frequently asked questions.

How to Use MAL-dPEG®24-NHS ester

Because NHS esters hydrolyze readily in water or aqueous buffer, the NHS ester end of the molecule must conjugate to a target molecule before conjugating the maleimide end of the molecule. At pH 7.0 – 7.5, NHS esters react optimally with free amines. However, NHS esters can react with free amines with pH as low as 6.0. As the pH increases, the hydrolysis rate of the NHS ester increases. Indeed, at pH 7 and 0°C, the NHS ester half-life is four (4) to five (5) hours in aqueous buffer, while at pH 8 and 25°C, the half-life of an NHS ester is one hour.[3] Thus, we strongly discourage storing MAL-dPEG®24-NHS ester, product number 10314, in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use.

The reaction of the maleimide end of MAL-dPEG®24-NHS ester, product number 10314, with a sulfhydryl proceeds best at pH 6.5 – 7.5. Conduct the conjugation at the lowest reasonable pH within this range. Above pH 7.5, free amines compete with free thiols at the maleimide reaction site, which can cause confusing results. Moreover, at higher pH values, the maleimide ring may open to form unreactive maleamic acid.[4] For details about maleimide-thiol reaction chemistry, please click here.

Uses of MAL-dPEG®24-NHS ester

The use of MAL-dPEG®24-NHS ester, product number 10314, has been published in numerous scientific paper and patents. The following list highlights some of the more important uses of this product:

  • targeted delivery of a cancer drug via polylysine dendrimers;
  • targeted delivery of drugs to the lungs;
  • development of a blood purification device using magnetic nanoparticles;
  • development of a targeted therapeutic system using superparamagnetic nanoparticles;
  • improved transfection of tumor cells by PEGylated liposomes;
  • development of immunosensors and biosensors;
  • investigation of an aptamer using atomic force microscopy;
  • attaching antibodies to atomic force microscopy probes;
  • development of molecular pincers;
  • improvement of a peptidic ligand used for capture and purification of IgM; and,
  • surface coating of nanoparticles.

What can you do with this product?

Please click the “Add to Cart” button now to order MAL-dPEG®24-NHS ester, product number 10314.

Application References:

[1] Hermanson, G. T. Chapter 6, Heterobifunctional Crosslinkers. In Bioconjugate Techniques, 3rd ed.; Academic Press: New York, NY, 2013; pp 299–340, specifically page 300. Many scientists engaged in bioconjugation work consider Greg Hermanson’s book to be the definitive reference on the subject. Click here now to read a review of Greg’s book and to purchase it.

[2] Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. In Bioconjugate Techniques, 3rd ed.; Academic Press: New York, NY, 2013; pp 787–838, specifically page 794.

[3] Hermanson, G. T. Chapter 3, The Reactions of Bioconjugation. In Bioconjugate Techniques; Academic Press: New York, NY, 2013; pp 229–258, specifically page 234.

[4] Hermanson, G. T. Chapter 6, op. cit., page 304.

Description

 

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in