Specs:

 SDS

Molecular Weight: 559.65; single compound

CAS: 1334177-95-5

Purity: > 97%

Spacers: dPEG® Spacer is 20 atoms and 23.1 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SPDP and LC-SPDP on pp. 286-288. See Greg’s extensive index on pp. 1192-1193 for references to a number and range of applications and their respective protocols.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Post-transcriptional regulation of the GASC1 oncogene with active tumor-targeted siRNA-nanoparticle. Sara Movassaghian, Claudia Hildebrandt, Yuran Xie, Rayna Rosati, Ying Li, Na Hyung Kim, Denise Conti, Sandro RP da Rocha, Zeng-Quan Yang, and Olivia M. Merkel. Molecular Pharmaceutics. 2016, May 25, 2016. DOI: 10.1021/acs.molpharmaceut.5b00948.

Targeted Delivery of siRNA to Transferrin Receptor Overexpressing Tumor Cells via Peptide Modified Polyethylenimine. Yuran Xie, Bryan Killinger, Anna Moszczynska, and Olivia M. Merkel. Molecules. 2016, 21 (1334) pp 1-16. October 10, 2016. DOI: 10.3390/molecules21101334.

BSA Hydrogel Beads Functionalized with a Specific Aptamer Library for Capturing Pseudomonas aeruginosa in Serum and Blood. Markus Krämer, Ann-Kathrin Kissmann, Heinz Fabian Raber, Hu Xing, Patrizia Favella, Ingrid Müller, Barbara Spellerberg, Tanja Weil, Dennis Kubiczek, Susanne Sihler, Ulrich Ziener, and Frank Rosenau. International Journal of Molecular Sciences. 2021. Volume 22, Issue 20. October 15, 2021. https://doi.org/10.3390/ijms222011118

Highly Stable Polymer Coating on Silver Nanoparticles for Efficient Plasmonic Enhancement of Fluorescence. Ryo Kato, Mitsuhiro Uesugi, Yoshie Komatsu, Fusatoshi Okamoto, Takuo Tanaka, Fumihisa Kitawaki, and Taka-aki Tano. ACS Omega. 2022. January 7, 2022. https://doi.org/10.1021/acsomega.1c06010

Additional Search Phrases: SPDP-dPEG4-NHS ester, PEG, dPEG, discrete PEG, polyethylene glycol, poly (ethylene glycol), Quanta, Quanta BioDesign, PEGylation reagent, SPDP, SPDP-PEG-acid, SPDP PEG acid, thiol reactive, amine reactive, cross link, crosslink, cross linker, crosslinker, cross linking, crosslinking, sulfhydryl, disulfide, N-succinimidyl, N-hydroxysuccinimide, NHS, NHS ester, active ester, PEG4, SPDP-PEG4-NHS ester, OPSS-PEG4-NHS , NHS-PEG4-SPDP, PEG4-SPDP, SPDP-PEG-NHS ester

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SPDP-dPEG®₄-NHS ester

$225.00 – $1,075.00

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PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

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Product#: 10374 Categories: , ,

TFP_Esters_Art2

SPDP-dPEG®4-NHS ester, product number 10374, is Quanta BioDesign’s water-soluble version of the immensely popular SPDP crosslinker. SPDP-dPEG®4-NHS ester is a heterobifunctional crosslinker. The N-hydroxysuccinimidyl (NHS) ester end of the molecule reacts with free amines. The opposite end is a 3-(2-pyridyldithio)-propionate functional group that reacts with sulfhydryl groups in thiol-containing molecules to form disulfide bonds. Between the two functional groups is an amphiphilic, single molecular weight, discrete polyethylene glycol (dPEG®) spacer.

What is dPEG®?

Quanta BioDesign’s amphiphilic dPEG® PEGylation reagents create water-soluble conjugates. Our dPEG® products have all the benefits of traditional PEG products (including water solubility, increased hydrodynamic volume, and non-immunogenicity) without the liability of dispersity, which creates difficulties in analyzing the reaction products. This link thoroughly explains the benefits of dPEG® technology. For answers to our most frequently asked questions, please click here.

Crosslinking with SPDP

Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.

Using SPDP-dPEG®4-NHS ester

SPDP-dPEG®4-NHS ester, product number 10374, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product will not cause the compounds to which it is conjugated to precipitate due to excessive hydrophobicity. Greg Hermanson’s Bioconjugate Techniques, 3rd edition, describes SPDP and related products (Chapter 6) and describes Quanta BioDesign’s dPEG® versions of these products (Chapter 18). Each of the chapters describing these crosslinkers contains detailed protocols for using them. Quanta BioDesign highly recommends and sells this book. Click here for a review and to purchase Bioconjugate Techniques, 3rd edition.

Bulk Scale Production is Available

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

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If you need to conjugate amine and sulfhydryl groups, quit using inferior hydrophobic SPDP products. Click on the “Add to Cart” button today and start using our superior, water-soluble SPDP dPEG® product. You, too, can see “the dPEG® difference.”

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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