Specs:

 SDS

Molecular Weight: 912.08; single compound

CAS: 924280-65-9

Purity: > 97%

Spacers: dPEG® Spacer is 44 atoms and 51.3 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SPDP and LC-SPDP on pp. 286-288. See Greg’s extensive index on pp. 1192-1193 for references to a number and range of applications and their respective protocols.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Ellman's and Aldrithiol Assay as Versatile and Complementary Tools for the Quantification of Thiol Groups and Ligands on Nanomaterials. Marko Moser, Ralf Schneider, Thomas Behnke, Thomas Schneider, Jana Falkenhagen, and Ute Resch-Genger. Analytical Chemistry. 2016, pp 1-9. July 2, 2016. DOI: 10.1021/acs.analchem.6b01798.

Refined purification strategy for reliable proteomic profiling of HDL 2/3: Impact on proteomic complexity. Michael Holzer, Sabine Kern, Ruth Birner-Grunberger, Sanja Curcic, Akos Heinemann, and Gunther Marsche. Scientific Reports. 2016, 6 (38533) pp 1-10. December 5, 2016. DOI: 10.1038/srep38533.




Additional Search Phrases:

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 SDS    Datasheet

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SPDP-dPEG®₁₂-NHS ester

$250.00 – $1,200.00

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PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses
Product#: 10378 Categories: ,

TFP_Esters_Art2

SPDP-dPEG®12-NHS ester, product number 10378, is Quanta BioDesign’s water-soluble version of the immensely popular SPDP crosslinker. SPDP-dPEG®12-NHS ester is a heterobifunctional crosslinker. The N-hydroxysuccinimidyl (NHS) ester end of the molecule reacts with free amines. The opposite end is a 3-(2-pyridyldithio)-propionate functional group that reacts with sulfhydryl groups in thiol-containing molecules to form disulfide bonds. Between the two functional groups is an amphiphilic, single molecular weight, discrete polyethylene glycol (dPEG®) spacer.

The dPEG® Difference in Crosslinkers

Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. The crosslinking product N-succinimidyl 3-(2-pyridyldithio)-propionate, known commonly by the acronym SPDP, is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.

Quanta BioDesign’s amphiphilic dPEG® products create water-soluble conjugates. Our dPEG® products have all the benefits of traditional PEG products (including water solubility, increased hydrodynamic volume, and non-immunogenicity) without the liability of dispersity, which creates difficulties in analyzing the reaction products. For a thorough explanation of dPEG® technology, please click this link. For answers to our most frequently asked questions, please click here.

Using SPDP-dPEG®12-NHS ester in Bioconjugation Reactions

SPDP-dPEG®12-NHS ester, product number 10378, is used like SPDP and related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product does not cause the compounds to which it is conjugated to precipitate due to excessive hydrophobicity. The long spacer arm may facilitate the reaction of the SPDP end of the molecule with buried thiol groups.

Greg Hermanson’s Bioconjugate Techniques, 3rd edition, describes SPDP and related products (Chapter 6) and describes Quanta BioDesign’s dPEG® versions of these products (Chapter 18). Each of the chapters describing these crosslinkers contains detailed protocols for using them. Quanta BioDesign highly recommends and sells this book. Click here now for a review and to purchase Bioconjugate Techniques, 3rd edition.

Bulk Quantities of SPDP-dPEG®12-NHS ester Are Available

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

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Stop using inferior hydrophobic SPDP products to crosslink amine and sulfhydryl groups. Click on the “Add to Cart” button today and start using our superior, water-soluble SPDP dPEG® product. You, too, can see “the dPEG® difference.”

Description

 

 

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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