Bis-MAL-dPEG®11, product number 10397, is a medium-length, homobifunctional, crosslinking reagent that links two molecules (e.g., peptides, proteins) together via the thiol-maleimide reaction (also known as the thiol-Michael addition). The ends of the molecule are both functionalized with maleimidopropionate groups. The two reactive ends are separated by a single molecular weight, discrete polyethylene glycol (dPEG®) spacer that is 37 atoms (46.9 – 47.9 Å) long. The end-to-end length of the molecule is 49 atoms (62.3 – 63.3 Å).
The thiol-maleimide reaction, a type of click chemistry reaction, is an extremely popular way to conjugate molecules. The maleimide functional group rapidly reacts with sulfhydryl groups, and the reaction is chemoselective for sulfhydryls in the pH range of 6.5 – 7.5. For details about the maleimide-thiol reaction, please visit our Maleimide Reaction Chemistry page. The polyethylene glycol (PEG) spacer between the two maleimide groups is a single molecular weight compound with a discrete chain length (thus the tradename dPEG®). This product is not made from a disperse polymer but is instead a single compound. For more information about Quanta BioDesign’s dPEG® technology, please click this link. Applications for this product include, among many others, studying cell internalization of crosslinked proteins; developing FRET immunoassays; and anchoring biomolecules to surfaces.
If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.
Hermanson, G. T. Chapter 3, The Reactions of Bioconjugation. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, pages 229 – 258, particularly page 241, where Greg writes about the maleimide reactive group. Click here now for a review of Greg’s book and a link to purchase it.
Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, pages 787 – 838.
Johnson, P. A.; Levicky, R. Polymercaptosiloxane Anchor Films for Robust Immobilization of Biomolecules to Gold Supports. Langmuir2003, 19(24), 10288–10294. https://doi.org/10.1021/la035102s.
Johnson, P. A.; Levicky, R. X-Ray Photoelectron Spectroscopy and Differential Capacitance Study of Thiol-Functional Polysiloxane Films on Gold Supports. Langmuir2004, 20(22), 9621–9627. https://doi.org/10.1021/la048458s.
Ohiro, Y.; Ueda, H.; Shibata, N.; Nagamune, T. Enhanced Fluorescence Resonance Energy Transfer Immunoassay with Improved Sensitivity Based on the Fab′-Based Immunoconjugates. Analytical Biochemistry2007, 360(2), 266–272. https://doi.org/10.1016/j.ab.2006.10.025.
Capraro, B. R.; Shi, Z.; Wu, T.; Chen, Z.; Dunn, J. M.; Rhoades, E.; Baumgart, T. Kinetics of Endophilin N-BAR Domain Dimerization and Membrane Interactions. J. Biol. Chem.2013, 288(18), 12533–12543. https://doi.org/10.1074/jbc.M112.435511.
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