Specs:

 SDS

Molecular Weight: 564.58; single compound

CAS: 1204834-00-3

Purity: > 98%

Spacers: dPEG® Spacer is 28 atoms and 32.2 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

A Modular Platform for the Rapid Site-Specific Radiolabeling of Proteins with F Exemplified by Quantitative Positron Emission Tomography of Human Epidermal Growth Factor Receptor 2. Herman S. Gill, Jeff N. Tinianow, Annie Pgasawara, Judith E. Flores, Alexander N. Vanderbilt, Helga Raab, Justin M. Scheer, Richard Vandlen, Simon-P. Williams, and Jan Marik, J. Med. Chem., 2009, 52 (19), pp 5816–5825 September 9, 2009. DOI: 10.1021/jm900420c.

An Effective Targeted Nanoglobular Manganese(II) Chelate Conjugate for Magnetic Resonance Molecular Imaging of Tumor Extracellular Matrix. Mingqian Tan, Xueming Wu, Eun-Kee Jeong, Qianjin Chen, Dennis L. Parker, and Zheng-Rong Lu,Mol. Pharmaceutics, 2010, 7 (4), pp 936–943 May 19, 2010. DOI: 10.1021/mp100054m.

Constant-speed vibrational signaling along polyethylene glycol chain up to 60-Å distance. Zhiwei Lin and Igor V. Rubtsov. PNAS, 2012, 5 (109), pp 1413-1418, January 31, 2012. DOI:10.1073/pnas.1116289109.

Ballistic energy transport along PEG chains: distance dependence of the transport efficiency. Zhiwei Lin , Nan Zhang , Janarthanan Jayawickramarajah and Igor V. Rubtsov. Physical Chemistry Chemical Physics. 2012, 30 (14), pp 10445-10454. April 12, 2012. DOI: 10.1039/C2CP40187H.

Enzymatic Ligation of Large Biomolecules to DNA. Rasmus Schøler Sørensen, Anders Hauge Okholm, David Schaffert, Anne Louise Bank Kodal, Kurt V. Gothelf, and Jørgen Kjems. ACS Nano. 2013, 7 (9) pp 8098–8104. August 8, 2013. DOI: 10.1021/nn403386f.

Click Chemistry Immobilization of Antibodies on Polymer Coated Gold Nanoparticles. Chiara Finetti, Laura Sola, Margherita Pezzullo, Davide Prosperi, Miriam Colombo, Benedetta Riva, Svetlana Avvakumova, Carlo Morasso, Silvia Picciolini, and Marcella Chiari. Langmuir. 2016, 32, pp 7435-7441. July 1, 2016. DOI: 10.1021/acs.langmuir.6b01142.

Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer. Felipe Opazo, Laura Eiden, Line Hansen, Falk Rohrbach, Jesper Wengel, Jørgen Kjems, and Günter Mayer. Molecular Therapy Nucleic Acids. 2015, 4 pp e251. 9/1/2015. DOI: 10.1038/mtna.2015.25.


Additional Search Phrases:

Azido-dPEG8-NHS ester, Azido-N-Hydroxysuccinimide, NHS-Azide, 1204834003, PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, azide, azide ion, azido, azido group, click chemistry, click reagent, Huisgen cycloaddition, alcohol, hydroxy, PEG spacer, amine reactive, peptide formation, crosslinker, cross linker, crosslinking, crosslinking reagent, active ester, NHS ester, N-hydroxysuccinimide, Azido-PEG8-NHS ester, N3-PEG8-NHS, NHS-PEG8-azide, Azido-PEG-NHS ester



 SDS    Datasheet

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Azido-dPEG®₈-NHS ester

$200.00 – $650.00

Clear
PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses
Product#: 10503 Categories: ,

TFP_Esters_Art2

Azido-dPEG®8-NHS ester, product number 10503, is a water-soluble crosslinker used for click chemistry. This product contains an azide group linked to an N-hydroxysuccinimidyl (NHS) ester through a single molecular weight, discrete polyethylene glycol (dPEG®) spacer. Azido-dPEG®8-NHS ester works with copper(I)-catalyzed or ruthenium-catalyzed click chemistry and with copper-free click chemistry using Quanta BioDesign’s line of DBCO-functionalized dPEG® products. The dPEG® spacer imparts water solubility and adds hydrodynamic volume to the conjugated product. The single molecular weight product design, with its discrete chain length, simplifies the analysis of this product.

dPEG® Products are a Better Type of PEG

Quanta BioDesign’s single molecular weight, discrete chain length PEG (dPEG®) products are a superior alternative to traditional polyethylene glycol products. Conventional PEGylation reagents consist of a complex mixture of different chain lengths due to the polymerization processes that form them. In contrast, each of Quanta BioDesign’s dPEG® products contain a discrete chain length of PEG with only one molecular weight. Conjugates made with dPEG® products have less complicated analyses compared to conjugates prepared with traditional PEGylation reagents. For a more thorough discussion on dPEG® products, please see our “What is dPEG®?” page. For answers to our most frequently asked questions, please visit our “Frequently Asked Questions” page.

NHS Esters

NHS esters are the most popular, most widely used way to conjugate carboxylic acids to primary or secondary amines resulting in stable amide bonds. NHS esters react quickly and efficiently in aqueous media at physiological pH values (7.0 – 7.5). However, they are prone to hydrolysis at a rate that is pH-dependent.

Published research shows that 2,3,5,6-tetrafluorophenyl (TFP) esters are more hydrolytically stable and have better reactivity with amines than NHS esters. Work conducted internally by Quanta BioDesign, confirms the findings in the scientific literature.  For more information, please click TFP Esters Have More Hydrolytic Stability and Greater Reactivity Than NHS Esters.

Click Chemistry

  1. Barry Sharpless and colleagues defined the rapid, chemoselective, stereospecific reactions between an azide and an alkyne leading to the formation of a triazole ring as click chemistry. From its publication in 2001, click chemistry has grown consistently in popularity and importance for the development of new chemical structures.

The first-reported click chemistry reactions were catalyzed by copper(I) and are known as Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). Subsequently, copper-free click chemistry (formally known as strain promoted azide-alkyne cycloaddition, or SPAAC) was developed by Carolyn Bertozzi and colleagues to facilitate click chemistry reactions in living cells without the use of toxic copper salts. For more information, please see Click Chemistry with dPEG® Reagents.

Quanta BioDesign offers many click chemistry reagents, including a broad array of azide-functionalized dPEG® products and dPEG® products functionalized with dibenzyl cyclooctyne (DBCO) for SPAAC. Click this link to see all of our click chemistry products.

Bulk Scale Production is Available for Azido-dPEG®8-NHS ester

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

What Are You Waiting For?

If you have been using hydrophobic reagents or conventional PEGylation reagents, then you are in for a pleasant surprise! Our dPEG® reagents provide all the benefits of traditional PEGylation reagents without the analytical headaches that are common with ordinary polymer PEG products. Our products are water-soluble and non-immunogenic.

Stop using inferior reagents! Buy Azido-dPEG®8-NHS ester today and discover the dPEG® difference. To get started, click the “Add to Cart” button.

Application References:

  1. Hermanson, G. T. Chapter 3, The Reactions of Bioconjugation. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, pp 229-258, especially pages 233-234 (NHS esters) and pages 238-239 (fluorophenyl esters). Click here now for a review of Greg’s book and a link to purchase it.
  2. Hermanson, G. T. Chapter 17, Chemoselective Ligation; Bioorthogonal Reagents. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, pp 757-786, particularly pages 769-775 where click chemistry is discussed.
  3. Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, pp 787-838.
  4. Kolb, H. C.; Finn, M. G.; Sharpless, K. B. Click Chemistry: Diverse Chemical Function from a Few Good Reactions. Angew. Chem. Int. Ed., 2001, 40, 2004-2021. https://doi.org/10.1002/1521-3773(20010601)40:11%3C2004::AID-ANIE2004%3E3.0.CO;2-5
  5. Kolb, H. C.; Sharpless, K. B. The growing impact of click chemistry on drug discovery. Drug Disc. Today, 2003, 8(24), 1128-1137. https://doi.org/10.1016/S1359-6446(03)02933-7.
  6. Baskin, J. M.; Bertozzi, C. R. Bioorthogonal Click Chemistry: Covalent Labeling in Living Systems. QSAR & Combinatorial Science 2007, 26(11–12), 1211–1219. https://doi.org/10.1002/qsar.200740086.
  7. Patterson, D. M.; Nazarova, L. A.; Prescher, J. A. Finding the Right (Bioorthogonal) Chemistry. ACS Chem. Biol. 2014, 9(3), 592–605. https://doi.org/10.1021/cb400828a.
  8. Dommerholt, J.; Rutjes, F. P. J. T.; van Delft, F. L. Strain-Promoted 1,3-Dipolar Cycloaddition of Cycloalkynes and Organic Azides. Top. Curr. Chem. (Z) 2016, 374(2), 16. https://doi.org/10.1007/s41061-016-0016-4.
  9. Johansson, J. R.; Beke-Somfai, T.; Said Stålsmeden, A.; Kann, N. Ruthenium-Catalyzed Azide Alkyne Cycloaddition Reaction: Scope, Mechanism, and Applications. Chem. Rev. 2016, 116(23), 14726–14768. https://doi.org/10.1021/acs.chemrev.6b00466.
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Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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