Fmoc-N-Lys-(dPEG®4-biotin)-OH, product number 10613, allows the incorporation of a short, biotinylated discrete PEG (dPEG®) into a peptide chain using standard Fmoc chemistry with Fmoc-protected lysine. The biotin-dPEG®4 moiety is positioned on the side chain of lysine. By design, this product facilitates the incorporation of a water-soluble biotin label into a peptide chain.
Peptide PEGylation with dPEG® Products
PEGylation is the process of adding polyethylene glycol (PEG) to a peptide, protein, carbohydrate, or surface (for example, gold, silver, metal, silica, or plastic). PEG is amphiphilic, meaning that it is soluble in both water and many organic solvents. Thus, PEGylation imparts water solubility to molecules to which it is conjugated.
Peptide synthesis often uses PEGylation to improve the water solubility of hydrophobic peptides. Also, PEGylation protects peptides from enzymatic degradation in serum and increases the PEGylated peptide’s hydrodynamic volume. A peptide’s serum half-life increases with increasing hydrodynamic volume.
To learn more about dPEG® technology, please visit our “What is dPEG®?” page. To get answers to our frequently asked questions, please click here.
Biotinylation is the process of incorporating biotin into a molecule, such as a peptide, protein, carbohydrate, or small molecule drug, or onto a surface. Biotin binds the chicken egg protein avidin and the bacterial protein streptavidin with high affinity. Indeed, the biotin-avidin and biotin-streptavidin bonds are among the strongest non-covalent bonds currently known. Poorly soluble in water, biotin becomes highly water-soluble when conjugated to a short, discrete PEG linker. Because the dPEG®4 spacer adds hydrophilicity to the peptide, there will be fewer concerns about insolubility, aggregation, and precipitation than there would be if using biocytin or LC-biotin.
To learn more about biotinylation with dPEG® products, please visit our Biotinylation with dPEG® Products page.
Fmoc-N-Lys-(dPEG®4-biotin)-OH uses familiar Fmoc chemistry with either solid phase or solution phase peptide synthesis. Although most customers will want to put the biotinyl-lysine residue on one end of a peptide chain, the biotin label can be incorporated into the peptide chain wherever it is convenient to do so. Additional dPEG® linkers can be incorporated into the peptide as needed using Quanta BioDesign’s extensive list of peptide modification products.
Commercial Scale Production Is Available for Fmoc-N-Lys-(dPEG®4-biotin)-OH
If you need bulk amounts of this product in a package size larger than our standard sizes, please contact us for a quote. Our commercial capabilities allow us to manufacture this product at any scale that you need.
Hydrophobic LC-biotin reagents are known to be problematic in bioconjugation work. So, stop using inferior biotinylation reagents! You can do better. Quanta BioDesign offers better biotinylation tools. Our dPEG®-based biotinylation reagents offer water solubility, improved hydrodynamic volume, no background noise (which means better signal), and no protein precipitation caused by aggregation. Why would you not use something better?
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