Specs:

 SDS

Molecular Weight: 695.71; single compound

CAS: N/A

Purity: > 98%

Spacers: dPEG® Spacers are 25 and 29 atoms and 17.5 and 27.9 Å, resp.

Shipping: Ambient

References: Mono-PEGylated Dimeric Exendin-4 as High Receptor Binding and Long-Acting Conjugates for Type 2 Anti-Diabetes Therapeutics.Tae Hyung Kim, Hai Hua Jiang, Seulki Lee, Yu Seok Youn, Chan Woong Park,Youngro Byun, Xiaoyuan Chen, and Kang Choon Lee, Bioconjugate Chem., 2011, 22 (4), pp 625–632 March 14, 2011. DOI: 10.1021/bc100404x.




Additional Search Phrases:

Bis-MAL-Lysine-dPEG4-acid, Bis-mal, PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, crosslinker, cross linker, crosslinking reagent, thiol, thiol reactive, maleimide, maleimido, bis-maleimido, bis-MAL, lysine, PEG4, acid, amine reactive, Bis-MAL-Lysine-PEG4-acid



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Bis-MAL-Lysine-dPEG®₄-acid

$225.00 – $1,050.00

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PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses
Product#: 10630 Categories: ,

Bis-MAL-Lysine-dPEG®4-acid, product number 10630, is a unique crosslinker from Quanta BioDesign, Ltd. The two amines of lysine are functionalized with maleimidopropyl groups that react with sulfhydryl groups. The carboxylic acid tail of lysine is conjugated to a single molecular weight, discrete polyethylene glycol (dPEG®) spacer containing a carboxylic acid terminus that can be reacted with free amines or used to provide negative surface charge. The distance from the carbonyl carbon of the dPEG® spacer to the reactive site of the α-amine-conjugated maleimide group is 25 atoms (17.5 Å) long. The distance from the carbonyl carbon of the dPEG® spacer to the reactive site of the ε-amine-conjugated maleimide group is 29 atoms (27.9 Å) long.

PEGylation with dPEG® Products

Unlike traditional polyethylene glycol (PEG), which is a disperse polymer (Ð > 1), all of Quanta BioDesign’s dPEG® products are synthesized as single molecules. Consequently, our dPEG® products create conjugates with no dispersity. These conjugates exhibit a more straightforward analysis compared to traditional polymer PEG products. For more information about dPEG® products, please visit this page. To get answers to frequently asked questions, please click here.

Using Bis-MAL-Lysine-dPEG®4-acid

By design, the two maleimide groups of Bis-MAL-Lysine-dPEG®4-acid can be used to bridge disulfides. For example, this capability may be useful in antibodies following reduction of the disulfide groups. Moreover, the two maleimide groups can be used to dimerize small molecules or peptides, each of which possesses a single free thiol group.

At pH 6.5 – 7.5, maleimide groups react chemoselectively with sulfhydryls. However, above pH 7.5, chemoselectivity is lost, as maleimide groups also will react with free amines.

For more information about maleimide chemistry and reactivity, please read Maleimide Reaction Chemistry.

The carboxylic acid end of the molecule couples with free amines using EDC chemistry to form stable amide bonds. However, if the acid terminus of the molecule is left unreacted, the acid can act as a charge modifier for the molecule to which it is conjugated. Construction of antibody-drug conjugates (ADCs); immobilization of antibodies and antibody fragments; and dimerization of sulfhydryl-containing peptides are some of the possible uses for this product.

Commercial Scale Production

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Act Now

To get started, please click the “Add to Cart” button now. Buy Bis-MAL-Lysine-dPEG®4-acid and discover the difference that dPEG® products make in peptide synthesis. Please click “Add to Cart” now.

Application References:

  1. Hermanson, G. T. Chapter 3, The Reactions of Bioconjugation. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, pages 229 – 258, particularly page 241, where Greg writes about the maleimide reactive group. Want to learn more about Greg’s book? Click here now for a review of this remarkable reference work and a link to purchase it.
  2. Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, pages 787 – 838.
  3. Kim, T. H.; Jiang, H. H.; Lee, S.; Youn, Y. S.; Park, C. W.; Byun, Y.; Chen, X.; Lee, K. C. Mono-PEGylated Dimeric Exendin-4 as High Receptor Binding and Long-Acting Conjugates for Type 2 Anti-Diabetes Therapeutics. Bioconjugate Chem. 2011, 22(4), 625–632. https://doi.org/10.1021/bc100404x.

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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