dPEG®₄₈-biotin acid

$300.00 – $1,500.00

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PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

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Product#: 10776 Categories: Amine Reactive Biotinylation Reagents, Biotinylation Reagents, dPEG®x-biotin acid

Product number 10776, dPEG^®₄₈ biotin acid, is a 2,500 Dalton biotin acid product that contains a dPEG^® spacer. Because this product is a free acid rather than an active ester, it can be functionalized for a variety of uses.

Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or to a surface such as glass or gold. It is widely used in bioconjugation research and product development. Biotin is normally poorly soluble in water, but the amphiphilic dPEG^® linker imparts excellent solubility in water or aqueous buffer and in organic solvent. In the past, many experiments have coupled aminocaproic acid to biotin to use as a spacer between biotin and the surface to be modified. Unfortunately, aminocaproic acid (often abbreviated as LC, for “long chain”) is highly hydrophobic. Insoluble in water, LC-biotin must be dissolved in an organic solvent such as DMF or DMSO before using in aqueous buffer. When conjugated to proteins or other biomacromolecules, LC-biotin can trigger aggregation and precipitation of conjugated molecules due to hydrophobic interactions. In assays, the hydrophobicity of LC-biotin also increases background noise through non-specific binding.

Amphiphilic dPEG^®₄₈ biotin acid can be dissolved both in water or aqueous buffer and in organic solvent. It does not cause aggregation or precipitation of biomolecules. Using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry, PN10776 can be coupled directly to free primary amines in aqueous media. These free amines can be on a protein, peptide, or the treated surface of a nanoparticle. Moreover, the acid moiety can be functionalized with other reactive groups for coupling directly to surfaces such as glass, gold, or magnetic nanoparticles. The nearly 180Å linker length of dPEG^®₄₈ biotin acid is far longer than the linker lengths of LC-biotin and of LC-LC-biotin. The performance characteristics of dPEG^®₄₈-biotin acid are quite superior to both LC-biotin and LC-LC-biotin.

This product has been used in a variety of applications that take advantage of the strong avidin-biotin binding interaction. Such applications include characterizing protein-protein interactions, developing sensitive biosensors, measuring cytokine responses to inflammation, and functionalizing surfaces with dPEG^®₄₈ biotin.

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Application References:

Hermanson, G. T. Chapter 11, (Strept)avidin-Biotin Systems. Bioconjugate Techniques, 3^rd edition. Academic Press: New York, 2013, 465-505. Click here for a review of Greg’s book and a link to purchase it.
Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 787-838.