Specs:

 SDS

Molecular Weight: 796.97; single compound

CAS: 956494-20-5

Purity: > 98%

Spacers: dPEG® Spacer is 40 atoms and 50.4 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0), and specifically see pp. 722-724.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Cardiovascular Small Heat Shock Protein HSPB7 Is a Kinetically Privileged Reactive Electrophilic Species (RES) Sensor. Sanjna L. Surya, Marcus J. C. Long, Daniel A. Urul, Yi Zhao, Emily J. Mercer, Islam M. Elsaid, Todd Evans, and Yimon Aye. ACS Chemical Biolody. 2018, 13, pp 1824-1831. February 3, 2018. DOI: 10.1021/acschembio.7b00925.

Site-Specific Incorporation of Photo-Cross-Linker and Bioorthogonal Amino Acids into Enteric Bacterial Pathogens. Shixian Lin, Zhenrun Zhang, Hao Xu, Lin Li, She Chen, Jie Li, Ziyang Hao, and Peng R. Chen. J. Am. Chem. Soc. 2011, 133 (50), pp 20581–20587. November 15, 2011. DOI: 10.1021/ja209008w.

Precision Electrophile Tagging in Caenorhabditis elegans. Marcus J. C. Long, Daniel A. Urul, Shivansh Chawla, Hong-YuLin, Yi Zhao, Joseph A. Haegele, Yiran Wang, and Yimon Aye. Biochemistry. 2018, 57 (2), pp 216-220. August 31, 2017. DOI: 10.1021/acs.biochem.7b00642.

Binding of the Treslin-MTBP Complex to Specific Regions of the Human Genome Promotes the Initiation of DNA Replication. Akiko Kumagai, William G. Dunphy. Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. 2020. Volume 32, Issue 12. 9/22/2020. http://doi.org/10.1016/j.celrep.2020.108178

Function-guided proximity mapping unveils electrophilic-metabolite sensing by proteins not present in their canonical locales. Yi Zhao, Pierre A Miranda Herrera, Dalu Chang, Romain Hamelin, Marcus J C Long, Yimon Aye. PNAS Biochemistry. 2022. January 26, 2022. https://doi.org/10.1073/pnas.2120687119

Additional Search Phrases:

Biotin-dPEG11-azide, azido, n3, 956494205, PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, biotin, biotinylation, biotinylate, biotin label, biotin labeling, biotin azide, azide, azido, azide ion, azido group, click chemistry, click reagent, Huisgen cycloaddition, Biotin-PEG11-azide, Biotin-PEG11-N3 Azido-PEG11-biotin, PEG500 biotin azide, Biotin-PEG-azide



 SDS    Datasheet

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Biotin-dPEG®₁₁-azide

$250.00 – $1,400.00

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PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses
Product#: 10784 Categories: , , ,

Biotin-dPEG®11-azide, product number 10784, is part of a family of biotinylation products designed for use in azide-alkyne click chemistry reactions. This product enables biotinylation of molecules using either copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC), ruthenium-catalyzed azide-alkyne cycloaddition (RuAAC), or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Consequently, these products allow new types of biotinylated constructs.

Discrete PEG Products

Quanta BioDesign invented dPEG® technology. Our single molecular weight polyethylene glycol products have no dispersity (Ð = 1). In contrast, conventional PEG products typically have relatively large dispersity values (Ð = 1.05 – 1.2). For complete information on dPEG® products and technology, please visit our “What is dPEG®?” page. For answers to our frequently asked questions, please click this link.

Biotinylation

Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or a surface such as glass or gold. The process is used widely in bioconjugation research and product development. Although biotin is poorly soluble in water, the conjugation of biotin to an amphiphilic dPEG® linker imparts excellent solubility in aqueous and organic solvents. For a thorough discussion of biotinylation with discrete PEG products, please visit our page “Biotinylation with dPEG® products.”

Using Biotin-dPEG®11-azide

Biotin-dPEG®11-azide is designed for click chemistry reactions. Catalytic amounts of copper(I) or ruthenium or highly-strained cyclooctyne reagents such as dibenzylcyclooctyne (DBCO) promote a rapid reaction between the azide and an alkyne group to form a triazole moiety.

Through protein engineering, non-natural amino acids with alkyne side chains can be incorporated into specific locations on proteins, thereby enabling reproducible biotinylation of proteins. Likewise, synthetic peptides containing amino acids with alkyne side chains allow azide-alkyne click chemistry-driven biotinylation. Furthermore, surfaces functionalized with alkyne groups can be biotinylated using click chemistry. Moreover, this product can be modified with other products from Quanta BioDesign to create new compounds tailored to your specific needs. For additional information on click chemistry, please visit our page Click Chemistry with dPEG® Reagents.

As a biotinylation reagent, Biotin-dPEG®11-azide works in various applications. Pull-down assays, affinity purification, plate-type assays such as ELISA, and supramolecular construction can all incorporate Biotin-dPEG®11-azide. For instance, in a novel use of PN10784, Biotin-dPEG®11-azide was employed in a pull-down assay to identify electrophiles in signaling pathways in the worm Caenorhabditis elegans.

Because Biotin-dPEG®11-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®11-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.

Products Related to Biotin-dPEG®11-azide

Biotin-dPEG®11-azide is just one member of a family of Biotin-dPEG®-azide products. Please see the complete family of Click dPEG® Biotinylation Reagents. Also, we offer several DBCO-functionalized dPEG® products as SPAAC partners for our Click dPEG® Biotinylation Reagents. Please click this link to see our DBCO-based dPEG® reagents.

Commercial Scale Production Is Available for Biotin-dPEG®11-azide

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Act Now

Stop using conventional biotinylation reagents! You can do better. Our dPEG® biotinylation reagents offer water solubility, improved hydrodynamic volume, no background noise (which means better signal), and no protein precipitation caused by aggregation. Why would you not use something better?

For cleaner click chemistry crosslinking, click the “Add to Cart” button now. You will not regret it. Click “Add to Cart” now.

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