Biotin-dPEG®₂₃-NH₂

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Biotin-dPEG®23-NH2 (Biotin-dPEG®23-amine), product number 10786, is one of several carbonyl- and carboxylic acid-reactive biotinylation products offered by Quanta BioDesign, Ltd. The dPEG®23 spacer is 71 atoms long (87.0 Å) with 23 oxygen atoms in the single molecular weight PEG chain. Biotin-dPEG®23-NH2 reacts with activated esters (N-hydroxysuccinimide, 2,3,5,6-tetrafluorophenyl) of carboxylic acids under slightly basic conditions. Moreover, it can be coupled directly to a carboxylic acid using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry. Additionally, the product reacts with aldehydes or ketones to form a Schiff base. The Schiff base can be reduced to a secondary amine using sodium cyanoborohydride if additional conjugate stability is necessary.

Biotinylation and dPEG® Products

For a general discussion of biotinylation with dPEG® products, please visit our page “Biotinylation with dPEG® Products.” To learn more about Quanta BioDesign’s dPEG® technology, please visit our “What is dPEG®?” page. To find answers to the questions that we are most frequently asked, please click this link.

Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or a surface such as glass or gold. Biotinylation features prominently in bioconjugation research and product development. Biotin usually is poorly soluble in water. However, conjugates that incorporate Biotin-dPEG®23-NH2 display excellent solubility in aqueous media and organic solvents.

In the past, there have not been many choices for carbonyl- and carboxylic acid-reactive biotinylation reactions. The most popular choices were biotin-hydrazide, biocytin-hydrazide (biotin-lysine hydrazide), and biotin-LC-hydrazide (biotin-aminocaproic acid hydrazide), but all of these choices are unfortunately quite hydrophobic. Insoluble, or at least poorly soluble, in water these older, hydrophobic reagents required dissolution in a water-miscible organic solvent such as DMF or DMSO before using in aqueous media. When conjugated to proteins or other biomacromolecules, the hydrophobic interactions caused or exacerbated by these reagents triggered aggregation and precipitation of conjugated molecules and increased background noise through non-specific binding.

Using Biotin-dPEG®23-NH2

Amphiphilic Biotin-dPEG®23-NH2 dissolves equally well in both aqueous media and organic solvents. Therefore, it is unnecessary to dissolve this product in an organic solvent before using it in an aqueous reaction. Furthermore, biotinylation of biomacromolecules with Biotin-dPEG®23-NH2 will not cause aggregation and precipitation. Eliminating aggregation and precipitation improves the signal-to-noise ratio of applications developed with this product. Reactions with carboxylic acids or active esters of carboxylic acids yield stable amide bonds. Aldehydes are not commonly found in biomacromolecules but can be formed by oxidizing reducing sugars in glycosylated proteins. Reactions with aldehydes create imines that can be reduced under mild conditions to secondary amines with sodium cyanoborohydride.

Biotin-dPEG®23-NH2 works in any application that can take advantage of the exceptionally strong biotin-avidin/streptavidin affinity. The terminal amine of PN10786 offers the opportunity (1) to biotinylate the carbohydrate coat of glycoproteins by oxidizing reducing sugars to aldehydes and (2) to biotinylate surfaces consisting of carboxylic acids or their active esters. Thus, this product expands the range of molecules and nanoparticles in which dPEG®-biotin can be employed.

Commercial Scale Production Is Available for Biotin-dPEG®23-NH2

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Related Products

This product is one of several related Biotin-dPEG®-amine products with varying lengths of dPEG® spacers. These additional products can be found here. We also offer related biotinylation products as hydrazides (to form hydrazone bonds) and oxyamines (to form oxime bonds).

Act Now

Stop using inferior biotinylation reagents! Sure, many other people use LC-biotin products, but you can do better. Our biotinylation reagents offer water solubility, improved hydrodynamic volume, no background noise (which means better signal), and no protein precipitation caused by aggregation. Why would you not use something better?

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Additional information

Weight.5 oz
Dimensions.75 × .75 × 2 in