m-dPEG®₁₂-Thiol

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m-dPEG®12-thiol, PN 10794, is a monodisperse PEGylation reagent used to modify silver or gold nanoparticle surfaces and sulfhydryl-containing molecules. The single molecular weight, discrete-length polyethylene glycol (dPEG®) chain is 38 atoms long (44.2 Å). A non-reactive methyl group caps one end of the dPEG® chain, while a free thiol terminates the opposite end. The terminal thiol group forms disulfide bonds with other thiols, dative bonds with gold and silver, and thioether bonds with maleimides and bromoacetates.

PEGylation with dPEG® products

PEGylation increases water solubility and expands the hydrodynamic volume of conjugates. Also, PEGylation reduces immunogenicity, renal clearance, and non-specific interactions arising from hydrophobic regions on surfaces and biomolecules. Surface PEGylation prevents proteins and other biomolecules from binding non-specifically with the surface.

Traditional PEGylation reagents are disperse polymers. Such products consist of a variety of PEG chain lengths and molecular weights. When reacted with biomolecules, this complex mixture of PEG products leads to a muddled combination of conjugates that are difficult and time-consuming to analyze.

In contrast, Quanta BioDesign’s dPEG® products are monodisperse PEG products. Because these products are synthesized from high purity starting materials, they have no dispersity (Ð = 1). Since there is a single PEG product, the conjugates formed are predictable and relatively straightforward to analyze.

Learn more about our dPEG® products!

What is dPEG®?

Frequently Asked Questions (about dPEG® products)

Using m-dPEG®12-thiol

m-dPEG®12-thiol reacts readily in non-oxidizing environments. In aqueous solution, care must be taken to exclude oxygen from the water by bubbling nitrogen or helium through the water. With biomolecules, it may be prudent to add a small amount of ethylenediaminetetraacetic acid (EDTA) to chelate any heavy metals that might be present as these could also promote oxidation. Greg Hermanson’s text, Bioconjugate Techniques, 3rd edition, contains protocols for reacting thiols with gold, silver, and biomolecules.

This product is suitable for passivating surfaces of gold or silver and for modifying biomolecules to improve performance and solubility. In scientific literature, m-dPEG®12-thiol was used to probe the structure and reactivity of CRM197, a carrier protein used in vaccine development.

Bulk quantities are available

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

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Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in