Biotin-dPEG®₇-azide

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Biotin-dPEG®7-azide, product number 10825, is part of a family of biotinylation products designed for azide-alkyne click chemistry reactions. These reactions require catalytic amounts of copper(I) or ruthenium or the use of highly strained cyclooctyne compounds such as dibenzylcyclooctyne (DBCO). This family of products enables the biotinylation of molecules using chemistry that is different from traditional amide bond formation. Consequently, these products permit new types of biotinylated constructs.

What are dPEG® Products?

Quanta BioDesign invented dPEG® technology. Our single molecular weight polyethylene glycol products have no dispersity (Ð = 1). In contrast, conventional PEG products typically have relatively large dispersity values (Ð = 1.05 – 1.2). For complete information on dPEG® products and technology, please visit our “What is dPEG®?” page. For answers to our frequently asked questions, please click this link.

Biotinylation

Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or a surface such as glass or gold. The process is used widely in bioconjugation research and product development. Although biotin is poorly soluble in water, the conjugation of biotin to an amphiphilic dPEG® linker imparts excellent solubility in aqueous and organic solvents. For a thorough discussion of biotinylation with discrete PEG products, please visit our page “Biotinylation with dPEG® products.”

Using Biotin-dPEG®7-azide

By design, Biotin-dPEG®7-azide works in all azide-alkyne cycloadditions. These reactions are catalyzed by copper(I) (CuAAC) or ruthenium (RuAAC) or promoted by strain (SPAAC) using DBCO or like reagents.

Through protein engineering, non-natural amino acids with alkyne side chains can be incorporated into specific locations on proteins, thereby enabling reproducible biotinylation of proteins. Likewise, synthetic peptides containing amino acids with alkyne side chains allow azide-alkyne click chemistry-driven biotinylation. Furthermore, surfaces functionalized with alkyne groups can be biotinylated using click chemistry. Moreover, this product can be modified with other products from Quanta BioDesign to create new compounds tailored to your specific needs. For additional information on click chemistry, please visit our page Click Chemistry with dPEG® Reagents.

As a biotinylation reagent, Biotin-dPEG®11-azide works in various applications. Pull-down assays, affinity purification, plate-type assays such as ELISA, and supramolecular construction can all incorporate Biotin-dPEG®7-azide.

Because Biotin-dPEG®7-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®7-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, the location of each conjugation sites is predictable.

Biotin-dPEG®7-azide and Related Products

Biotin-dPEG®7-azide is just one member of a family of Biotin-dPEG®-azide products. Please see the complete family of Click dPEG® Biotinylation Reagents. Also, we offer several DBCO-functionalized dPEG® products as SPAAC partners for our Click dPEG® Biotinylation Reagents. Please click this link to see our DBCO-based dPEG® reagents.

Commercial Scale Production Is Available for Biotin-dPEG®11-azide

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Act Now

Stop using conventional biotinylation reagents! You can do better. Our click chemistry biotinylation reagents offer water solubility, improved hydrodynamic volume, no background noise (which means better signal), and no protein precipitation caused by aggregation. Why would you not use something better?

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Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in