Amino-dPEG®₃₆-t-butyl ester



Amino-dPEG®36t-butyl ester, product number 10901, is a heterobifunctional, monodisperse polyethylene glycol (PEG) crosslinker that couples carboxylic acids to amines. One end of the crosslinker terminates with an amine-reactive, tert-butyl-ester-protected propionic acid. The t-butyl ester protecting group is cleavable with trifluoroacetic acid (TFA). Once cleaved, the exposed acid reacts with amines to form amide bonds. The opposite end terminates with a carbonyl-reactive primary amine.

Heterobifunctional Crosslinkers

Heterobifunctional crosslinkers join different reactive functional groups together through a crossbridge. Crosslinkers that join amines and carboxylates are particularly popular. However, conventional crosslinkers are often hydrophobic and may trigger aggregation and precipitation of conjugates that use them.

In contrast, Quanta BioDesign’s single molecular weight dPEG® crosslinkers are highly water-soluble amphiphiles. Consequently, dPEG® crosslinkers impart water solubility to conjugates that incorporate them. As a result, these crosslinkers are increasingly popular in bioconjugation applications. Moreover, our dPEG® products provide all of the benefits of traditional PEG products without the analytical headaches that inevitably arise from using disperse polymer products.

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What Is dPEG®?

Frequently Asked Questions (about dPEG® products)

Using Amino-dPEG®36t-butyl ester

The amine end of the molecule reacts with activated carboxylic acid esters (for example, N-hydroxysuccinimidyl or tetrafluorophenyl esters) to form an amide bond. Also, carboxylic acids can couple to the amine end of Amino-dPEG®36-t-butyl ester using a carbodiimide such as EDC, with or without an acylating agent such as N-hydroxysuccinimide (NHS) or 2,3,5,6-tetrafluorophenol (TFP).

After working up the reaction to remove unreacted components and isolate the intermediate, trifluoroacetic acid is used to remove the tert-butyl ester and expose the propionic acid end group. The exposed carboxylic acid can then react further. For example, activation as the NHS or TFP ester is possible. Also, the acid can be coupled directly to an amine as described above.

Commercial Scale Production of Amino-dPEG®24t-butyl ester Is Available

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

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Please click the “Add to Cart” button now to purchase this product. Once you start using our products, you will understand why we at Quanta BioDesign talk about “the dPEG® difference” when comparing our products to conventional crosslinkers and traditional PEG products.

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in