S-acetyl-dPEG®₁₂-OH

Bioconjugation frequently employs thiolation because the reactions to install thiol groups on molecules or to react with molecules containing sulfhydryl groups are relatively simple and often chemoselective. The thioacetyl group is intended to install an acetyl-protected thiol onto biomolecules. While installation of a protected thiol through a reaction between a carboxylic acid and a primary amine is widely popular in bioconjugation technology, it is not always possible or feasible to do. S-acetyl-dPEG^®₁₂-OH permits installation of a protected thiol through a short, hydroxy-terminated discrete PEG^® linker. Hydroxylamine hydrochloride (CAS number 5470-11-1) easily removes the acetyl group protecting the thiol, thus exposing the sulfhydryl for reaction. One application for S-acetyl-dPEG^®₁₂-OH is modification of nucleic acids using phosphoramidite chemistry. See reference 3, below. For more information about Quanta BioDesign’s thiolation reagents, please see Thiolation Reagents and Reactions. {link}

Traditional polyethylene glycol (PEG) products are dispersed (Đ ≥ 1) polymers containing an intractable mixture of different chain lengths and molecular weights of PEG in a Poisson distribution. Quanta BioDesign manufactures PEG products that are not dispersed (Đ = 1). The PEG contained in every PEG product made by Quanta BioDesign has a single molecular weight with a discrete chain length (hence the tradename dPEG^®). For more information about dPEG^® technology, please click here.

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

Application References:

1. Hermanson, G. T. Chapter 2, Functional Targets for Bioconjugation. Bioconjugate Techniques, 3^rd edition. Academic Press: New York, 2013, 127-228, particularly pages 165-170, discussing thiolation and SATA. Want to learn more about Greg’s book? Click here now for a review of Greg’s book and a link to purchase it.
2. Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 787-838.
3. Zhang, L.; Sun, L.; Cui, Z.; Gottlieb, R. L.; Zhang, B. 5′-Sulfhydryl-Modified RNA: Initiator Synthesis, in Vitro Transcription, and Enzymatic Incorporation. Bioconjugate Chem. 2001, 12(6), 939–948. https://doi.org/10.1021/bc015504g.