Specs:

 SDS

Molecular Weight: 1413.55; single compound

CAS: 1008402-79-6

Purity: > 97%

Spacers: dPEG® Spacer is 79 atoms and 93.0 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Multivalent Benzamidine Molecules for Plasmin Inhibition: Effect of Valency and Linker Length. Tanmaye Nallan Chakravarthula, Dr. Ziqian Zeng, Prof. Nathan J. Alves. ChemMedChem, Volume 17, Issue 22, (2022), 09/16/2022, https://doi.org/10.1002/cmdc.202200364.

Additional Search Phrases:

Bis-dPEG25-NHS ester, n-hydroxysuccinimide, 1008402796, PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, crosslinker, cross linker, crosslinking reagent, amine reactive, homobifunctional, NHS, N-hydroxysuccinimide, NHS ester, N-hydroxysuccinimide ester, active ester, Bis-PEG25-NHS ester, NHS-PEG25-NHS ester, PEG1000 bis-NHS ester, Bis-NHS-PEG



 SDS    Datasheet

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Bis-dPEG®₂₅-NHS ester

$350.00 – $1,000.00

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PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses
Product#: 10968 Categories: ,

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Bis-dPEG®25-NHS ester, product number 10968, is a monodisperse, homobifunctional, amine-reactive polyethylene glycol (PEG) crosslinker. Both ends of the dPEG® crosslinker terminate with N-hydroxysuccinimidyl (NHS) esters. NHS esters are presently the most widely used amine-reactive acylating reagents. This product crosslinks peptides and proteins with free amines such as the side chains of lysine. Consequently, Bis-dPEG®25-NHS ester is useful in proteomics research. Bis-dPEG®25-NHS ester increases the water solubility and hydrodynamic volume of conjugates made with it. Increased hydrodynamic volume decreases renal clearance.

Homobifunctional Reagents

Homobifunctional reagents are chemical compounds that contain two identical reactive groups. Symmetrical homobifunctional reagents were among the first crosslinkers developed for conjugation of biomolecules. These were typically hydrophobic compounds, and their use – particularly in one-step reactions – often was accompanied with problems such as inactivation and precipitation of the conjugated biomolecules. Greg Hermanson’s comprehensive reference work Bioconjugate Techniques has an entire chapter (Chapter 5) devoted to homobifunctional reagents. Quanta BioDesign, Ltd. recommends and sells this authoritative reference.

Quanta BioDesign’s products are amphiphilic. Therefore, they impart water solubility to molecules to which they are conjugated. Consequently, when used in crosslinking reactions, Bis-dPEG®25-NHS ester will not cause problems such as aggregation, precipitation, and inactivation.

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What is dPEG®?

Frequently Asked Questions (about dPEG® products)

Using Bis-dPEG®25-NHS ester

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.0. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases.

Thus, we strongly discourage storing Bis-dPEG®25-NHS ester, product number 10968, in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Commercial Scale Production of Bis-dPEG®25-NHS ester Is Available

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

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Hydrophobic crosslinkers trigger aggregation and precipitation of biomolecules and contribute to problems with non-specific binding. Polymeric PEG crosslinkers eliminate the issues caused by hydrophobic crosslinkers, but they create new, difficult challenges in analyzing and characterizing the crosslinked conjugates.

You can solve all of those problems right now by clicking on the “Add to Cart” button and purchasing Bis-dPEG®25-NHS ester. The monodispersed PEG crosslinker provides water solubility and larger hydrodynamic volume to the conjugate molecules. However, because it is a discrete compound, not a polymer, the analytical challenges caused by dispersed polymers disappear.

Stop using inferior crosslinkers!

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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