Fmoc-N-amido-dPEG®₄-TFP ester

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Fmoc-N-amido-dPEG®4-TFP ester, product number 11000, is a Fluorenylmethyloxycarbonyl-protected amino-dPEG®-acid that is pre-activated as the 2,3,5,6-tetrafluorophenyl (TFP) ester for efficient conjugation to amines. The 17 atoms-long (18.1 Å) dPEG®4 spacer allows the introduction of a short, hydrophilic spacer. Although designed for peptide synthesis, Fmoc-N-amido-dPEG®4-TFP ester can be used in numerous applications.

PEGylation with dPEG® Products

PEGylation refers to the covalent addition of polyethylene glycol (PEG) to a compound or surface. PEGylated compounds include proteins, peptides, dyes or other labels, and small molecule drugs.

PEG is amphiphilic. Consequently, peptide synthesis often employs PEGylation to enhance the water solubility of hydrophobic peptides. Moreover, PEGylation prolongs the in vivo circulation of peptide conjugates by increasing the peptide’s hydrodynamic volume and protecting the peptide from proteolysis. Furthermore, PEGylation reduces or eliminates the antigenicity of the conjugated peptide.

However, traditional PEGs are dispersed polymers (Đ > 1.00). A traditional PEG consists of multiple chain lengths with various molecular weights. The stated molecular weight for a conventional PEG indicates an average of the distribution of chain lengths and molecular weights.

Quanta BioDesign’s single molecular weight PEG products (known as “discrete PEG” and sold under the dPEG® trademark) provide all of the benefits of traditional, dispersed PEG without the analytical headaches that come from having an intractable mixture of conjugates, each with different PEG chain lengths.

Learn more about “the dPEG® difference” by clicking the links below!

What is dPEG®?

Frequently Asked Questions (about dPEG® products)

Using Fmoc-N-amido-dPEG®4-TFP ester

Fmoc-N-amido-dPEG®4-TFP ester permits our customers to insert a short (17 atoms) dPEG® linker into a peptide chain using well-known chemistry. The product works equally well in solid-phase and solution-phase synthetic processes. The dPEG® linker can be inserted at either end of a peptide chain or between two peptides to provide a flexible spacer. Also, in synthetic constructs where steric hindrance is a problem, the short dPEG® spacer can be used to moderate crowding. The amphiphilic nature of dPEG® means that the construct will gain some water solubility while remaining soluble in organic solvents. The Fmoc protecting group removes easily with a solution of piperidine in N, N-dimethylformamide (DMF).

Why Use TFP Esters Instead of NHS Esters?

Tetrafluorophenyl esters are generally superior to the more commonly used N-hydroxysuccinimidyl (NHS) esters. The 2,3,5,6-tetrafluorophenyl group is more stable to hydrolysis than NHS esters and more reactive to amines than NHS esters. Quanta BioDesign has additional information on why TFP esters are superior to NHS esters on our website.

Find the Right Product for Your Needs

We know that our customers have different needs for linker/spacer length. We also know that different customers will want different reactive end groups. To help our customers find the optimum linker length and reactive group for a particular product or application, we offer Fmoc-protected amino-dPEG® acids activated with either TFP or NHS. We also provide non-activated Fmoc-protected amino-dPEG® acids. We invite you to consider our entire line of peptide modification products and protected linkers whenever you need a single molecular weight PEG for your project.

Bulk Quantities Are Available

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

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