PEGylation with dPEG® Products
PEGylation refers to the covalent addition of polyethylene glycol (PEG) to a compound or surface. PEGylated compounds include proteins, peptides, dyes or other labels, and small molecule drugs.
Because PEG is amphiphilic, peptide synthesis often employs PEGylation to enhance the water solubility of hydrophobic peptides. Moreover, peptide PEGylation (1) prolongs the in vivo circulation of conjugates by increasing the conjugate’s hydrodynamic volume, (2) protects the peptide from proteolysis, and (3) reduces or eliminates the antigenicity of the conjugated peptide.
However, traditional PEGs are dispersed polymers (Đ > 1.00). A traditional PEG consists of multiple chain lengths with various molecular weights. The stated molecular weight for a conventional PEG indicates an average of the distribution of chain lengths and molecular weights.
Quanta BioDesign’s single molecular weight PEG products (known as “discrete PEG” and sold under the dPEG® trademark) provide all of the benefits of traditional, dispersed PEG without the analytical headaches that come from having an intractable mixture of conjugates, each with different PEG chain lengths.
Learn more about “the dPEG® difference” by clicking the links below!
Using Fmoc-N-amido-dPEG®8-TFP ester
Although we designed Fmoc-N-amido-dPEG®8-TFP ester for peptide synthesis, it is not limited to that specific use. This product permits our customers to conjugate a protected dPEG® amino acid to an amine. The product works equally well in solid-phase and solution-phase synthetic processes. The dPEG® can be inserted on the N-terminus of a growing peptide chain or in the middle of two peptides as a flexible spacer them. Also, the dPEG® chain can be used to provide distance in a synthetic construct where steric hindrance is a problem. The amphiphilic nature of dPEG® means that the construct gains water solubility while remaining soluble in organic solvents. The Fmoc protecting group removes easily with a solution of piperidine in N, N-dimethylformamide (DMF).
Why Use TFP Esters?
TFP esters are generally superior to the more commonly used N-hydroxysuccinimidyl (NHS) esters. The 2,3,5,6-tetrafluorophenyl group is more stable to hydrolysis more reactive to amines than NHS esters. Quanta BioDesign has additional information on why TFP esters are superior to NHS esters on our website.
Find the Right Product for Your Project
To help our customers discover the optimum dPEG® for a particular product or application, Fmoc-N-amido-dPEG®8-TFP ester, product number 11005, is part of a line of Fmoc-protected amino-dPEG® active ester products. We offer Fmoc-protected amino-dPEG® acids activated with either TFP or NHS. We also provide Fmoc-protected amino-dPEG® acids without TFP or NHS ester functionalization. We invite you to consider our entire line of peptide modification and protected linker reagents whenever you need a single molecular weight PEG for your project.
Bulk Quantities Are Available
If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.
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