Bis-Bromoacetamido-dPEG®11, product number 11338, is a thiol-reactive, homobifunctional crosslinker, with the two reactive ends separated by a single molecular weight, discrete polyethylene glycol (dPEG®) spacer that is 44 atoms long (47.5 Å). Both ends of the dPEG® spacer terminate with bromoacetyl groups that connect to the dPEG® cross-bridge through amide bonds.
Bromoacetyl-functionalized reagents are useful alternatives to maleimide-functionalized reagents. The bromoacetyl group reacts with sulfhydryl groups at pH 8 or higher. Maleimide groups are chemoselective for thiols at pH 6.5 – 7.5 and lose selectivity at higher pH values. Bromoacetyl-thiol reaction rates are somewhat slower than maleimide-thiol reaction rates. For more information about the bromoacetyl-thiol reaction, please see Bromoacetyl Reaction Chemistry.
Traditional polyethylene glycol (PEG) is a disperse polymer and consists of an intractable mixture of chain lengths and molecular weights. Thus, PEG molecular weights are averages of the polymeric mixture. Consequently, such products are challenging to analyze due to the distribution of chain lengths and molecular weights.
Quanta BioDesign’s dPEG® technology is quite different. Unlike traditional PEG, each of our dPEG® products consists of a single molecular weight product. Specifically, each dPEG® product has a discrete chain length (hence the dPEG® tradename). Therefore, our dPEG® products provide all of the benefits of PEG but are much less complicated to analyze and characterize. To learn more about our dPEG® technology, please click here. To get answers to our most frequently asked questions, please go to this page.
If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.
Hermanson, G. T. Chapter 2, Functional Targets for Bioconjugation. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 127-228. Want to learn more about Greg’s book? Click here now for a review of Greg’s book and a link to purchase it.
Hermanson, G. T. Chapter 3, The Reactions of Bioconjugation. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 229-258, specifically page 241, discussing maleimide reactions.
Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 787-838.