Specs:

 SDS

Molecular Weight: 1067.12; single compound

CAS: N/A

Purity: > 98%

Spacers: dPEG® Spacer is 40 atoms and 46.3 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

Additional Search Phrases:

PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, Quanta, Quanta BioDesign, PEGylation reagent. DBCO-PEG12-TFP ester, DBCO-PEG600-TFP ester, DBCO-PEG-TFP ester



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DBCO-dPEG®₁₂-TFP ester

$195.00 – $1,750.00

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PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses
Product#: 11366 Categories: , Tags: ,

DBCO-dPEG®12-TFP ester, product number 11366, is a heterobifunctional, bioorthogonal crosslinker. The tetrafluorophenyl (TFP) ester reacts specifically with free amines to form amide bonds. The dibenzylcyclooctyne (DBCO) moiety reacts selectively with azides via strain-promoted azide-alkyne cycloaddition (SPAAC) to form triazole linkages. A flexible, single molecular weight, discrete polyethylene glycol (dPEG®) spacer separates the functional groups at each end of the molecule. This dPEG® spacer increases the molecule’s hydrophilicity.

Click Chemistry and PEGylation

The published discovery in 2004 of SPAAC, also known as “copper-free click chemistry,” by Carolyn Bertozzi and coworkers, improved the click chemistry toolbox. Toxic copper catalysts were eliminated, making the reaction truly bioorthogonal. Because of the removal of copper from the reaction, SPAAC reagents can label living cells. (1) Since the original publication, multiple improvements in strained cyclooctyne reagents resulted in the development of DBCO as a nearly perfect azide partner for SPAAC. (2)

TFP Esters Are Superior to NHS Esters

TFP esters offer many advantages over traditional N-hydroxysuccinimide (NHS) esters. Research demonstrates that compared to NHS esters, TFP esters are more stable to hydrolysis in aqueous media and conjugate more efficiently to amines. (3) Quanta BioDesign also showed the superiority of TFP esters over NHS esters through in-house testing.

PEGylation with Discrete PEG (dPEG®) Products

Quanta BioDesign’s dPEG® reagents are superior to traditional, polymeric polyethylene glycol (PEG) reagents. Because they form from polymeric processes, conventional PEG reagents are dispersed polymers (Đ > 1). Disperse PEG contains a complex mixture of different chain lengths and molecular weights in a Poisson distribution. (4, 5) In contrast, each dPEG® reagent from Quanta BioDesign, Ltd contains a single PEG compound. (5) DBCO-dPEG®12-TFP ester simplifies the analysis of conjugates formed in the crosslinking reactions because the dPEG® crosslinker is a product with a discrete chain length and a single molecular weight.

Learn more!

What is dPEG®?

Frequently Asked Questions (about dPEG® products)

DBCO-dPEG®12-TFP ester, PN11366, is one of a series of crosslinkers, each with a different dPEG® chain length, containing DBCO and a TFP ester. To see all of our click chemistry reagents, please click here.

Bulk Quantities Are Available

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

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Application References:

  1. Agard, N. J.; Prescher, J. A.; Bertozzi, C. R. A Strain-Promoted [3 + 2] Azide−Alkyne Cycloaddition for Covalent Modification of Biomolecules in Living Systems. J. Am. Chem. Soc. 2004, 126(46), 15046–15047.
  2. Dommerholt, J.; Schmidt, S.; Temming, R.; Hendriks, L. J. A.; Rutjes, F. P. J. T.; vanHest, J. C. M.; Lefeber, D. J.; Friedl, P.; van Delft, F. L. Readily Accessible Bicyclononynes for Bioorthogonal Labeling and Three-Dimensional Imaging of Living Cells. Angewandte Chemie International Edition 2010, 49(49), 9422–9425.
  3. Wang, J.; Zhang, R.-Y.; Wang, Y.-C.; Chen, X.-Z.; Yin, X.-G.; Du, J.-J.; Lei, Z.; Xin, L.-M.; Gao, X.-F.; Liu, Z.; et al. Polyfluorophenyl Ester-Terminated Homobifunctional Cross-Linkers for Protein Conjugation. Synlett 2017, 28(15), 1934–1938. https://doi.org/10.1055/s-0036-1590974.
  4. Flory, P. J. Molecular Size Distribution in Ethylene Oxide Polymers. J. Am. Chem. Soc. 1940, 62(6), 1561–1565.
  5. Davis, P. D.; Crapps, E. C. (Quanta BioDesign, Ltd.). Selective and Specific Preparation of Discrete PEG Compounds. U.S. Patent 7,888,536, February 15, 2011.

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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