DBCO-dPEG®₂₄-TFP ester

Copper-Free Click Chemistry

The published discovery in 2004 of SPAAC, also known as “copper-free click chemistry,” by Carolyn Bertozzi and coworkers, improved the click chemistry toolbox. Toxic copper catalysts were eliminated, making the reaction truly bioorthogonal. SPAAC reagents permit the labeling of live cells due to the removal of copper from the reaction mixture. (1) Since the original publication, multiple improvements in strained cyclooctyne reagents resulted in the development of DBCO as a nearly perfect azide partner for SPAAC. (2)

TFP Esters vs. NHS Esters

TFP esters offer many advantages over traditional N-hydroxysuccinimide (NHS) esters. TFP esters are more stable to hydrolysis in aqueous media compared to NHS esters, even at higher pH ranges. Moreover, Quanta BioDesign has shown through in-house testing that at optimum pH, TFP esters label primary amines more readily than NHS esters. Published research by Wang, et al., support this finding. (3)

PEGylation with dPEG^® Reagents

Conventional PEG reagents are dispersed polymers (Đ > 1). Dispersed PEG polymers contain a complex mixture of different chain lengths and molecular weights in a Poisson distribution. (4, 5)

Quanta BioDesign’s dPEG^® reagents are superior to traditional, polymeric polyethylene glycol (PEG) reagents. In contrast to conventional PEG reagents, each dPEG^® reagent from Quanta BioDesign, Ltd contains a single PEG compound. (5) We synthesize and build up our dPEG^® reagents from high purity starting materials rather than by polymerization. Thus, our dPEG^® products are monodispersed (Ð = 1). The analysis of conjugates that contain dPEG^® linkers is simplified because the dPEG^® crosslinker is a product with a discrete chain length and a single molecular weight.

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What is dPEG^®?

Frequently Asked Questions (about dPEG^® products)

DBCO-dPEG^®₂₄-TFP ester, PN11370, is one of a series of crosslinkers, each with a different dPEG^® chain length, containing DBCO and a TFP ester. The other products in this series are:

PN11362, DBCO-dPEG^®₄-TFP ester; and

PN11366, DBCO-dPEG^®₁₂-TFP ester.

Bulk Quantities Are Available

If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.

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Application References:

1. Agard, N. J.; Prescher, J. A.; Bertozzi, C. R. A Strain-Promoted [3 + 2] Azide−Alkyne Cycloaddition for Covalent Modification of Biomolecules in Living Systems. J. Am. Chem. Soc. 2004, 126(46), 15046–15047.
2. Dommerholt, J.; Schmidt, S.; Temming, R.; Hendriks, L. J. A.; Rutjes, F. P. J. T.; van Hest, J. C. M.; Lefeber, D. J.; Friedl, P.; van Delft, F. L. Readily Accessible Bicyclononynes for Bioorthogonal Labeling and Three-Dimensional Imaging of Living Cells. Angewandte Chemie International Edition 2010, 49(49), 9422–9425.
3. Wang, J.; Zhang, R.-Y.; Wang, Y.-C.; Chen, X.-Z.; Yin, X.-G.; Du, J.-J.; Lei, Z.; Xin, L.-M.; Gao, X.-F.; Liu, Z.; et al. Polyfluorophenyl Ester-Terminated Homobifunctional Cross-Linkers for Protein Conjugation. Synlett 2017, 28(15), 1934–1938. https://doi.org/10.1055/s-0036-1590974.
4. Flory, P. J. Molecular Size Distribution in Ethylene Oxide Polymers. J. Am. Chem. Soc. 1940, 62(6), 1561–1565.
5. Davis, P. D.; Crapps, E. C. (Quanta BioDesign, Ltd.). Selective and Specific Preparation of Discrete PEG Compounds. U.S. Patent 7,888,536, February 15, 2011.