Specs:

 SDS

Molecular Weight: 3736.451; single compound

CAS: N/A

Purity: > 97%

Spacers: dPEG® Spacer data for each arm is 43 atoms and 47.4 Å

Shipping: Ambient

References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

Additional Search Phrases:

PEG, dPEG, discrete PEG, polyethylene glycol, PEGylation reagent, Quanta, Quanta BioDesign, PEGylation reagent, Tetra(-PEG11-DBCO)pentaerythritol, Pentaerythritol(-PEG11-DBCO)4, 4-arm PEG 2k DBCO



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Tetra(-dPEG®₁₁-DBCO)pentaerythritol

$1,200.00 – $2,300.00

Clear
PRODUCT IS SOLD STRICTLY FOR INTERNAL LABORATORY AND RESEARCH PURPOSES ONLY AND HAS NOT BEEN REVIEWED BY THE FDA. PRODUCT IS NOT FOR RESALE AND CANNOT BE INCORPORATED INTO COMMERCIAL GOODS FOR ANY USE OR USED IN THE DEVELOPMENT OF COMMERCIAL PRODUCTS OR IN THE PERFORMANCE OF COMMERCIAL SERVICES UNLESS UNDER A SEPARATE LICENSING, SUPPLY, OR DISTRIBUTOR AGREEMENT WITH QUANTA BIODESIGN, LTD. For information pertaining to the commercial use of our products, please click here to contact us.

Email Sales@QuantaBioDesign.com for Bulk Pricing and Custom Syntheses
Product#: 11371 Categories: , , Tags: , , , , , ,

Tetra(-dPEG®11-DBCO)pentaerythritol, product number 11371, is a four-armed, discrete polyethylene glycol (dPEG®) crosslinker built around a pentaerythritol core. Each arm terminates with a dibenzocyclooctyne (DBCO) functional group. Each arm conjugates to an azide-functionalized molecule or surface via strain-promoted azide-alkyne cycloaddition (SPAAC). This product is useful for supramolecular construction (e.g., dendrimers) and for efficiently crosslinking azide-functionalized proteins or peptides.

PEGylation and “the dPEG® Difference”

This compound showcases the advantages of dPEG® products. Conventional polyethylene glycol (PEG) products are dispersed polymers. These polymers consist of a complex mixture of different chain lengths and molecular weights that make exact analysis and characterization quite challenging.

Quanta BioDesign’s dPEG® products contain precisely defined, single molecular weight, discrete-length PEG chains (i.e., they are monodispersed PEG products). Tetra(‑dPEG®11-DBCO)pentaerythritol would be challenging to characterize if made with conventional PEG reagents because of polymer dispersity. The synthesis of a product like Tetra(‑dPEG®11-DBCO)pentaerythritol using standard PEG reagents creates a complicated assortment of different products. However, using dPEG® reagents, this product can be synthesized and characterized accurately. The clear advantage of dPEG® products is what we at Quanta BioDesign call “the dPEG® difference.”

The DBCO functional group at the end of each dPEG® chain consists of a strained cyclooctyne ring with benzyl rings fused on each side of the cyclooctyne. A glutaric acid group extends from the nitrogen atom on the cyclooctyne ring to permit conjugation to a primary amine. DBCO is a bioorthogonal, copper-free click chemistry reagent that reacts with azide groups through strain on the cyclooctyne ring, hence the acronym SPAAC. The strain-promoted click reaction produces a triazole ring fused to the eight-sided ring, with corresponding changes in bond hybridization. SPAAC is useful for reactions where Cu(I) cannot be used due to toxicity issues (for example, with living cells) or for reactions where copper would be difficult to remove in the workup of the product. For more information, please see Click Chemistry with dPEG® Reagents.

Related Product:

PN11372, Bis-dPEG®11-DBCO

Application References:

  1. Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, pp 787-838. Want to read a review of Greg’s book? Click here {link} for a review! Want to purchase a copy of Greg’s book? Click here now!
  2. Hermanson, G. T. Chapter 17, Chemoselective Ligation; Bioorthogonal Reagents. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, pp 757-786, particularly pages 769-775 where click chemistry is discussed.
  3. Hermanson, G. T. Chapter 8, Dendrimers and Dendrons. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 351-386.
  4. Baskin, J. M.; Bertozzi, C. R. Bioorthogonal Click Chemistry: Covalent Labeling in Living Systems. QSAR & Combinatorial Science 2007, 26(11–12), 1211–1219. https://doi.org/10.1002/qsar.200740086.
  5. Dommerholt, J.; Rutjes, F. P. J. T.; van Delft, F. L. Strain-Promoted 1,3-Dipolar Cycloaddition of Cycloalkynes and Organic Azides. Top. Curr. Chem. (Z) 2016, 374(2), 16. https://doi.org/10.1007/s41061-016-0016-4.

Additional information

Weight .5 oz
Dimensions .75 × .75 × 2 in

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