MAL-dPEG®4-Tris(TFP ester)3, product number 11416, is a unique crosslinking, monodisperse PEGylation reagent from Quanta BioDesign. This single molecular weight, discrete-length PEG (dPEG®) product permits one-to-three crosslinking of thiol and amine groups. The single maleimido group on one end of the dPEG® linker reacts with free thiols. On the opposite end of the molecule, a tris-based branching structure that terminates with propionic acid groups permits crosslinking with three different amino groups. The crosslinker length from the maleimido olefin to the terminal carbonyl carbon adjacent to the 2,3,5,6-tetrafluorophenyl (TFP) ester is 29 atoms (24.7 Å, avg.) long.
What are the uses of MAL-dPEG®4-Tris(TFP ester)3?
The well-known thiol-maleimide reaction permits conjugation of the maleimide group to free thiols. Because cysteine residues are comparatively rare in proteins, the thiol-maleimide reaction gives the user more control over the site(s) of conjugation. Scientists often make use of this reaction in the construction of antibody-drug conjugates (ADCs). The thiol-maleimide reaction is specific for free thiols at pH 6.5 – 7.5. However, specificity disappears above pH 7.5, as the maleimido group reacts with free amines such as the primary amine on the side chain of lysine. To learn more about the thiol-maleimide reaction, please visit our Maleimide Reaction Chemistry page.
The TFP ester reacts with free amines similarly to the more well-known N-hydroxysuccinimide (NHS) esters. Research, though, shows that TFP esters are more hydrolytically stable and reactive than NHS esters in aqueous media. Thus, TFP esters are a superior alternative to NHS esters. For more information on TFP esters, please click here.
The potential applications of MAL-dPEG®4-Tris(TFP ester)3 are limitless. Some possible uses for this product include the following:
Construction of dendrimers and other supramolecular constructs;
Creation of ADCs;
Modification of hydrogels with surface-accessible sulfhydryl groups; and,
Crosslinking small biomolecules such as peptides to create a targeted delivery vehicle.
Learn more about Quanta BioDesign’s dPEG® Technology!
Is MAL-dPEG®4-Tris(TFP ester)3 Available in Bulk Quantities?
Yes, it is. If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.
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This unique crosslinking PEGylation reagent from Quanta BioDesign offers all the benefits of traditional PEG products without the analytical complexity caused by dispersity. Our products have no dispersity. Each dPEG® product has a single molecular weight, discrete chain length PEG.
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