MAL-dPEG®12-Tris(TFP ester)3, product number 11427, is a unique, monodisperse, crosslinking PEGylation reagent from Quanta BioDesign. Slightly longer than the companion product, PN11416, this single molecular weight, discrete-length PEG (dPEG®) product permits one-to-three crosslinking of thiol and amine groups. The maleimido group on one end of the molecule reacts with free thiols. On the opposite end, a tris-based branching structure that terminates with propionic acid groups permits crosslinking with three different amino groups. The crosslinker length from the maleimido olefin to the terminal carbonyl carbon adjacent to the 2,3,5,6-tetrafluorophenyl (TFP) ester is 47 atoms (67.2 – 68.2 Å) long.
What is dPEG®?
Unlike conventional PEGylation reagents, monodisperse dPEG® products have a single molecular weight of PEG, and the PEG chain possesses a discrete length. There is no distribution of molecular weights and PEG chain lengths. Because dPEG® products are single molecules, the analysis and characterization of conjugates made with dPEG® products are enormously simplified compared to products made with dispersed PEG products. Quanta BioDesign sells our products under the dPEG® tradename to highlight the fact that we do not purify from a polymer mixture but synthesize them to the desired chain length starting from short, highly pure starting materials.
Learn more about Quanta BioDesign’s dPEG® Technology!
The well-known thiol-maleimide reaction conjugates a maleimide functional group to a free thiol such as is found on the side chain of the amino acid cysteine. Because proteins typically possess relatively few cysteine residues compared to other amino acids, the user gains control over the site(s) of conjugation. Scientists often use thiol-maleimide chemistry in the construction of antibody-drug conjugates (ADCs) because it is specific for free thiols at pH 6.5 – 7.5. However, specificity disappears above pH 7.5, as the maleimido group reacts with free amines such as the primary amine on the side chain of lysine. To learn more about the thiol-maleimide reaction, please visit our Maleimide Reaction Chemistry page.
The TFP ester reacts with free amines similarly to the more well-known N-hydroxysuccinimide (NHS) esters. Research, though, shows that TFP esters are more hydrolytically stable and reactive than NHS esters in aqueous media. Thus, TFP esters are a superior alternative to NHS esters. For more information on TFP esters, please click here.
The potential applications of MAL-dPEG®12-Tris(TFP ester)3 are limitless. Some possible uses for this product include the following:
Construction of dendrimers and other supramolecular constructs;
Creation of ADCs;
Modification of hydrogels with surface-accessible sulfhydryl groups; and,
Crosslinking small biomolecules such as peptides to create a targeted delivery vehicle.
Bulk Quantities of MAL-dPEG®12-Tris(TFP ester)3
If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.
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This unique crosslinking PEGylation reagent from Quanta BioDesign offers all the benefits of traditional PEG products without the analytical complexity caused by dispersity. Our products have no dispersity. Each dPEG® product has a single molecular weight, discrete chain length PEG.
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