References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.
Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.
Bromoacetamido-dPEG®12-Tris(-dPEG®11-amido-bromoacetamide)3, product number 11433, is a branched, single molecular weight, thiol-reactive, discrete polyethylene glycol (dPEG®) product consisting of four (4) branched dPEG® arms built around a tris core. Each arm terminates with a thiol-reactive bromoacetyl group. Furthermore, each arm is 42 atoms long (53.5 – 54.5 Å) from the tris core to the methylene group adjacent to the bromine atom.
Traditional polyethylene glycol (PEG) is a disperse polymer (Đ > 1). A traditional PEG of a stated molecular mass is actually an average mass drawn from a rather large number of different chain lengths of PEG, each chain having its own molecular mass. This dispersity makes analysis of bioconjugate products challenging.
In sharp contrast to traditional PEG, Quanta BioDesign’s dPEG® products have no dispersity (Đ = 1). Each dPEG® product is a single molecular weight compound. Moreover, we do not purify a single molecular weight of PEG out of a complex polymeric mixture. Rather, we build up our products from ultrapure starting materials and maintain uniform product purity throughout the synthetic, workup, and purification steps leading to the final product. For more information about our dPEG® technology, please click here. For answers to frequently asked questions, please click here.
The four arms of Bromoacetamido-dPEG®12-Tris(-dPEG®11-amido-bromoacetamide)3 are built from a tris(hydroxymethyl)aminomethane (“tris”) core. Although one arm is differently structured than the other three arms (one dPEG®12 arm vs three dPEG®11 arms), each arm contains 42 atoms and has the same length when measured from the methylene unit adjacent to the bromine atom to the tris core from which each arm branches. The discrete chain lengths of each dPEG® spacer enable such precise construction. Such precision is not possible with traditional, dispersed polymer PEG.
The bromoacetyl group reacts chemoselectively with thiols at pH ≥ 8.0 to form a stable thioether linkage. To learn more about the reaction of the bromoacetyl group with thiols, go to Bromoacetyl Reaction Chemistry.
If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.
Application References:
Hermanson, G. T. Chapter 2, Functional Targets for Bioconjugation. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 127-228, specifically pages 191-192 discussing bromoacetyl and iodoacetyl reactions with thiols. Click here now for a review of Greg’s book and a link to purchase it.
Hermanson, G. T. Chapter 3, The Reactions of Bioconjugation. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 229-258, specifically page 241, discussing maleimide reactions.
Hermanson, G. T. Chapter 8, Dendrimers and Dendrons. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 351-386.
Hermanson, G. T. Chapter 18, PEGylation and Synthetic Polymer Modification. Bioconjugate Techniques, 3rd edition. Academic Press: New York, 2013, 787-838.
We use cookies on our website to give you the most relevant experience by remembering your preferences and repeat visits. By clicking “Accept”, you consent to the use of ALL the cookies.
This website uses cookies to improve your experience while you navigate through the website. Out of these cookies, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may have an effect on your browsing experience.
Necessary cookies are absolutely essential for the website to function properly. This category only includes cookies that ensures basic functionalities and security features of the website. These cookies do not store any personal information.
Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. It is mandatory to procure user consent prior to running these cookies on your website.