References: Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.
Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.
Engineering the Orientation, Density, and Flexibility of Single-Domain Antibodies on Nanoparticles To Improve Cell Targeting. Ken W. Yong, Daniel Yuen, Moore Z. Chen, Angus P. R. Johnston. ACS Appl. Mater. Interfaces 2020, 12, 5, 5593-5600. January 9, 2020. DOI: 10.1021/acsami.9b20993
Biotin-dPEG®12-DBCO, product number 11811, labels azide-containing target molecules bioorthogonally using strain-promoted azide-alkyne cycloaddition (SPAAC), also known as “copper-free click chemistry.” A linear, single molecular weight, discrete polyethylene glycol (dPEG®) spacer separates the biotin and dibenzylcyclooctyne (DBCO) groups on either end of the molecule. The DBCO functional group reacts with any available azide moiety on a target molecule via SPAAC. The amphiphilic dPEG® spacer imparts water solubility to the product.
Biotinylation
The covalent addition of biotin to a molecule (biotinylation) is one of the most common methods of labeling molecules, such as proteins and peptides, for protein capture, affinity purification, pull-down assay, immunoprecipitation, and enzyme-linked immunosorbent assay (ELISA). The binding affinity of biotin for avidin or streptavidin is one of the strongest known non-covalent bonds. Biotin usually is poorly soluble in water, but the addition of amphiphilic polyethylene glycol (PEG) imparts water solubility to biotin.
For more information on biotinylation with dPEG® products, please click here.
Many Different Biotinylation Products Are Available
We have many dPEG®-based biotinylation products. For a complete list of our biotinylation products, please click here.
Copper-Free Click Chemistry (aka, SPAAC)
Carolyn Bertozzi and coworkers developed SPAAC (copper-free click chemistry) as an alternative to the copper-catalyzed azide-alkyne cycloaddition (CuAAC) developed by K. Barry Sharpless and coworkers. SPAAC avoids the use of copper, which is toxic to living cells. Thus, SPAAC is considered a bioorthogonal reaction that can label living cells. Azides are generally not present in biomolecules, but several methods exist for introducing azide groups into them. The combination of SPAAC via Biotin-dPEG®12-DBCO, PN11811, with azides introduced into biomolecules, allows the user a high degree of control over the labeling process.
To see our complete line of click chemistry products, please click here.
Quanta BioDesign’s dPEG® Products
Traditional PEG products are dispersed polymers (Đ > 1), consisting of a Poisson distribution of various chain lengths, each with a different molecular weight. Each of Quanta BioDesign’s discrete PEG (dPEG®) products contains a single PEG chain length with one molecular weight (Đ = 1). Our dPEG® products are much simpler to analyze than traditional PEG products because our products do not contain the complex mixture of PEG chains found in traditional PEG products.
If you need bulk product in a larger package size than our standard sizes, please contact us for a quote. Our commercial capabilities permit us to manufacture this product at any scale that you need.
Click “Add to Cart” Now to Purchase
To purchase Biotin-dPEG®12-DBCO, please click the “Add to Cart” button now.
Kolb, H. C.; Finn, M. G.; Sharpless, K. B. Click Chemistry: Diverse Chemical Function from a Few Good Reactions. Angew Chem Int Ed, 2001, 40, 2004-2021.
Kolb, H. C.; Sharpless, K. B. The growing impact of click chemistry on drug discovery. Drug Disc Today, 2003, 8(24), 1128-1137.
Baskin, J. M.; Bertozzi, C. R. Bioorthogonal Click Chemistry: Covalent Labeling in Living Systems. QSAR & Combinatorial Science2007, 26(11–12), 1211–1219.
Beatty, K. E.; Fisk, J. D.; Smart, B. P.; Lu, Y. Y.; Szychowski, J.; Hangauer, M. J.; Baskin, J. M.; Bertozzi, C. R.; Tirrell, D. A. Live-Cell Imaging of Cellular Proteins by a Strain-Promoted Azide–Alkyne Cycloaddition. ChemBioChem2010, 11(15), 2092–2095.
Robinson, P. V.; de Almeida-Escobedo, G.; de Groot, A. E.; McKechnie, J. L.; Bertozzi, C. R. Live-Cell Labeling of Specific Protein Glycoforms by Proximity-Enhanced Bioorthogonal Ligation. J. Am. Chem. Soc.2015, 137(33), 10452–10455.
Zheng, M.; Zheng, L.; Zhang, P.; Li, J.; Zhang, Y. Development of Bioorthogonal Reactions and Their Applications in Bioconjugation. Molecules2015, 20(2), 3190–3205.
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