Biotin-dPEG®12-DBCO, product number 11811, labels azide-containing target molecules bioorthogonally using strain-promoted azide-alkyne cycloaddition (SPAAC), also known as “copper-free click chemistry.” A linear, single molecular weight, discrete polyethylene glycol (dPEG®) spacer separates the biotin and dibenzylcyclooctyne (DBCO) groups on either end of the molecule. The DBCO functional group reacts with any available azide moiety on a target molecule via SPAAC. The amphiphilic dPEG® spacer imparts water solubility to the product.
The covalent addition of biotin to a molecule (biotinylation) is one of the most common methods of labeling molecules, such as proteins and peptides, for protein capture, affinity purification, pull-down assay, immunoprecipitation, and enzyme-linked immunosorbent assay (ELISA). The binding affinity of biotin for avidin or streptavidin is one of the strongest known non-covalent bonds. Biotin usually is poorly soluble in water, but the addition of amphiphilic polyethylene glycol (PEG) imparts water solubility to biotin.
For more information on biotinylation with dPEG® products, please click here.
Many Different Biotinylation Products Are Available
We have many dPEG®-based biotinylation products. For a complete list of our biotinylation products, please click here.
Copper-Free Click Chemistry (aka, SPAAC)
Carolyn Bertozzi and coworkers developed SPAAC (copper-free click chemistry) as an alternative to the copper-catalyzed azide-alkyne cycloaddition (CuAAC) developed by K. Barry Sharpless and coworkers. SPAAC avoids the use of copper, which is toxic to living cells. Thus, SPAAC is considered a bioorthogonal reaction that can label living cells. Azides are generally not present in biomolecules, but several methods exist for introducing azide groups into them. The combination of SPAAC via Biotin-dPEG®12-DBCO, PN11811, with azides introduced into biomolecules, allows the user a high degree of control over the labeling process.
To see our complete line of click chemistry products, please click here.
Quanta BioDesign’s dPEG® Products
Traditional PEG products are dispersed polymers (Đ > 1), consisting of a Poisson distribution of various chain lengths, each with a different molecular weight. Each of Quanta BioDesign’s discrete PEG (dPEG®) products contains a single PEG chain length with one molecular weight (Đ = 1). Our dPEG® products are much simpler to analyze than traditional PEG products because our products do not contain the complex mixture of PEG chains found in traditional PEG products.
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Zheng, M.; Zheng, L.; Zhang, P.; Li, J.; Zhang, Y. Development of Bioorthogonal Reactions and Their Applications in Bioconjugation. Molecules2015, 20(2), 3190–3205.
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