|Dimensions||.75 × .75 × 2 in|
$29.00 – $94.00
The dPEG® Biotin Quantification Assay is used to determine biotinyation levels in labeled antibodies and other proteins. The ready to use format includes a premixed solution of avidin and our dPEG®ylated HABA (4′-hydroxyazobenzene-2-carboxylic acid) dye designed to enhance solubility in solution. The ready to use format requires a spectrophotometer or plate reader capable of measuring absorbance at 500nm. When a biotinylated antibody solution is mixed with the dPEG® HABA Avidin solution, the biotin displaces the HABA and the absorbance at 500nm decreases. The biotin concentration is calculated from the change in absorbance using the exctinction coefficient of the dPEG® HABA Avidin complex. For the best results, use the dPEG® Biotin Quantification Assay with antibodies and proteins labeled with dPEG® biotinylation reagents.