Quanta BioDesign’s exclusive Biotinidase Resistant Biotin reagents create unique application opportunities for discerning scientists. Biotinidase is a ubiquitous mammalian enzyme that cleaves biotin from biocytin, making the free cofactor available to biotin-dependent enzymes such as carboxylases. However, biotinidase complicates applications such as antibody pretargeting with a biotin-streptavidin system because it attacks the targeting system’s biotin.
In 2006, D. Scott Wilbur, Donald K. Hamlin, and Ming-Kuan Chyan discovered a small biotin modification that resists biotinidase cleavage. Biotin binding to avidin or streptavidin remains unchanged. Our four amine-reactive Biotinidase Resistant Biotin products build on this discovery and enable new applications that expand the utility of the tight binding between biotin-avidin and biotin-streptavidin.
If your biotin-based application needs to evade biotinidase, try these products today and discover the dPEG® difference!
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