Q-Bright ECL Plus is more sensitive than many of the standard, commercial ECL reagents and is useful for routine western blots designed to detect abundant proteins. The Q-Bright ECL Plus Chemiluminescent Detection Kit provides a strong signal for abundant proteins.

Comparing Sensitivities

Here, we demonstrate the differing sensitivities with identical primary and secondary antibody concentrations and imaging time. The western blotting conditions can be further optimized for maximal signal and signal to noise by adjusting the antibody concentrations and exposure times. HeLa cell lysate was loaded at 10-1.21ug/well and transferred to a PVDF membrane. The membrane was incubated with Rabbit anti-Beta-Catenin (1:10,000) followed by Goat anti-Rabbit Horseradish Peroxidase (HRP) secondary antibody with minimal cross-reactivity to murine, human, bovine and horse IgGs (AQ-30049 1:20,000). Finally, the membranes were incubated with Q-Bright ECL Plus or a commercial ECL reagent for 5 minutes then imaged together on a digital chemiluminescent imaging system.

Q-Bright Endure Plus and Q-Bright Extreme provide the highest levels of sensitivity

Use the most sensitive Q-Bright chemiluminescent reagents to detect low abundant proteins or smaller quantities of starting material. Q-Bright Endure Plus provides great sensitivity without extensive optimization of western blotting conditions that is required when working with the most sensitive of reagents. The sensitive reagents conserve resources by using less primary and secondary antibody.