Schematic drawing of a TOPO coated green quantum dot, approximately 3 nm in diameter.

dPEG® Eliminates Non-Specific Binding on Quantum Dots

What about Quantum Dots?

Let’s start with a quick refresher. Quantum dots are cool little tiny nano particles, less than 10 nm in diameter, made of a semiconductor alloy core and coated with a shell of a different alloy, which fluoresce at different wavelengths depending on their size and specific composition. The smaller the quantum dot, the more blue-shifted its emission wavelength while larger quantum dots (6 nm and larger) shift to red and near-IR. Quantum dots can be a good alternative to organic dyes since they have broad excitation spectra (absorb energy at a wide range of wavelengths), narrow emission spectra (emit a fairly specific wavelength), and don’t suffer from photobleaching (degradation due to light intensity/oxidizing agents). Read more about fluorescence excitation and emission.

Issues with Quantum Dots

Water solubility is one of the major hurdles that must be overcome when producing quantum dots for biological applications. A standard method of making quantum dots involves injecting solutions of the semiconductor metals into hot (>300°C) organic solvent such as octadecene, and allowing the metals to nucleate (the first step of crystallization) and form alloys. Commonly used alloys are CdSe, CdTe, InP for emission wavelengths of 470-720nm and PbS and PbSe for >900nm. The reaction time is directly related to the size of the resulting quantum dots. When the quantum dots have reached their desired size, the surface is coated (modified) so that they are easily isolated. Originally this was done using tri-n-octylphosphine oxide (TOPO) which results in a very hydrophobic quantum dot (Figure 1).

Schematic drawing of a TOPO coated green quantum dot, approximately 3 nm in diameter.
Figure 1: TOPO coated green quantum dot (approximately 3 nm diameter)

At this point, the TOPO must be replaced with a water soluble group or more hydrophilic group (this is where dPEG® comes in handy). The TOPO is commonly replaced by a thiol containing linker such as 2-mercaptoacetic acid, lipoic acid (also known as thioctic acid), 2-mercaptoethylamine (2-MEA), or cystamine. These compounds are shown in Figure 2.

 

Figure 2: Commonly used sulfur-containing compounds used for attachment to gold surfaces such as quantum dots.
Figure 2: Commonly used sulfur-containing compounds for attachment to gold surfaces such as quantum dots.

While these modifications allow for increased hydrophilicity, the water solubility can be increased even more by adding a dPEG® to the acid or amine end by a simple coupling using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). Alternatively, a thiol-dPEG® could also be used directly on the surface of the quantum dot. Figure 3 shows dPEG® compounds attached to a quantum dot either directly (as one of Quanta BioDesign’s thiol or lipoamide products) or coupled to a thiol monomer already on the surface.

Figure 3: Red Quantum Dot with Attached dPEG® Compounds
Figure 3: Red Quantum Dot (approximately 6 nm diameter) with Attached dPEG® Compounds m-dPEG®8-lipoamide (PN10800), m-dPEG®8-acid (NHS ester) (PN10324, PN10260 – coupled to cystamine) and m-dPEG®8-amine (PN10278 – coupled to Mercaptoacetic acid)

The YouTube video below was published by CEN Online in 2011 and is about seven and one-half minutes long. It demonstrates how quantum dots are made.

 

dPEG® Solves Quantum Dot Problems

Elizabeth L. Bentzen, et al., working in the lab of Sandra J. Rosenthal at Vanderbilt University, studied the effects of PEG on nonspecific binding associated with quantum dots and found that PEG as small as PEG350 (approximately dPEG®8), when coated over the surface of quantum dots can reduce nonspecific binding in certain cells. The results showed that, in the cells studied, the PEG length could possibly be shortened to 12 units without increasing the nonspecific binding. Using a dPEG® in place of a traditional polydispersed PEG gives the advantages of lot-to-lot reproducibility, as well as confident identification of final product.

Products

See all of our metal surface modification dPEG® reagents here. For the products referenced in the caption to Figure 2, above, click on the links below:

PN10800, m-dPEG®8-lipoamide

PN10324, m-dPEG®8-acid

PN10260, m-dPEG®8-NHS ester

PN10278, m-dPEG®8-amine

References

Surface Modification To Reduce Nonspecific Binding of Quantum Dots in Live Cell Assays. Bioconjugate Chemistry (2005), 16, 1488-1494. Elizabeth L Bentizen, et al. Department of Chemistry, Vanderbilt University, Station B, 351822, Nashville, Tennessee 37235-1822, USA http://www.ncbi.nlm.nih.gov/pubmed/16287246

Mortimer, Mortimer, and Michael W. Davidson. “Overview of Fluorescence Excitation and Emission Fundamentals.” Olympus Microscopy Resource Center. http://www.olympusmicro.com/primer/lightandcolor/fluoroexcitation.html

Bioconjugate Techniques, Third Edition. Greg T. Hermanson. pages 455-463. Purchase here (free with orders of $1200 or more).

 

Bryan Davis is a Process Development Chemist at Quanta BioDesign, Ltd.