Thiol reactive crosslinkers are one of the most common classes of crosslinkers in bioconjugation (1). Because most proteins contain far more free amines than thiols and disulfides, conjugation to a thiol allows for greater control of the conjugation. Even more control of the conjugation process is afforded if a thiol reactive compound is combined with an amine reactive compound to create a heterobifunctional crosslinker. Continue reading →